Bimetallic organic framework-based aptamer sensors: a new platform for fluorescence detection of chloramphenicol

Lu, Zijing; Jiang, Yansong; Wang, Peng; Xiong, Wenhui; Qi, Bao‐Ping; Zhang, Yingkun; Ding, Xiang; Kun, Zhai

doi:10.1007/s00216-020-02737-y

Cited by 22 publications

(11 citation statements)

References 47 publications

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“…The biosensor used in this study only consisted of one aptamer and two split probes. The preparation was easy and the requirements for storage conditions were lower compared with ternary Ag2CrO4/g-C3N4/GO nanocomposite 15 , fluorochrome 16 , labeled oligonucleotide 17 , bimetallic organic framework 18 , GO-QDs nanocomposites and enzyme-linked aptamers 19 , and gold nanoparticles 20 in previous studies. The simple reaction system of our assay also resulted in a lower cost, about 0.16 dollars per reaction (the cost included oligonucleotide synthesis, reagents, and consumables).…”

Section: Detection Limit and Linear Detection Range Of The Biosensormentioning

confidence: 96%

“…Thus, they show good potential in the detection of pathogens 13 , metal ions 14 and antibiotics including CAP 10 . As with antibodies, aptamers targeting CAP always combine with other indicators or catalytic labels such as ternary Ag2CrO4/g-C3N4/GO nanocomposite 15 , fluorochrome 16 , labeled oligonucleotide 17 , bimetallic organic framework 18 , GO-QDs nanocomposites 19 , and gold nanoparticles 20 . However, they are still insufficiently sensitive, complex in preparation or dependent on specifical equipment.…”

Section: Introductionmentioning

confidence: 99%

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A visual on-site biosensor for low-cost detection of chloramphenicol based on aptamer and split DNAzyme

Chen

et al. 2022

ANAL. SCI.

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Chloramphenicol (CAP) is a kind of broad-spectrum antibiotics, which has been forbidden in food by most countries because of its side effects. In this study, a simple and low-cost biosensor for CAP detection in food was developed. The biosensor only consisted of an aptamer specific to CAP and a pair of split probes that could self-assemble as DNAzyme. The detection result could be identified by the naked eye and the visual limit was 10 nM CAP. The absorbance of final reaction products at 417 nm had a linear relationship with the logarithm of the CAP concentration ranged from 10 to 200 nM, and the limit of detection was 87.3 pM. The visual analysis by imageJ also showed a linear detection range between 25 and 200 nM. The whole detection could be finished in about 1.5 hours with about 0.16 dollars per reaction. We believe that the biosensor shows great potential in the rapid and sensitive detection of CAP in food.

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Section: Detection Limit and Linear Detection Range Of The Biosensormentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

A visual on-site biosensor for low-cost detection of chloramphenicol based on aptamer and split DNAzyme

Chen

et al. 2022

ANAL. SCI.

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“…Relying on the strong adsorption and fluorescence quenching ability of MOF, two dye-based fluorescent sensing strategies were explored for CAP. , Lu et al successfully prepared the bimetallic organic framework nanomaterial Cu/UiO-66 by coordinate bonding of MOF UiO-66 with Cu 2+ and established a fluorescence method for the quantitative detection of CAP using phosphate and ROX double-labeled aptamers and Cu/UiO-66 . The method displayed low fluorescence background, good reproducibility, and high sensitivity with LOD of 0.09 nM (30 pg mL –1 ).…”

Section: Application Of Fluorescent Aptasensor For the Detection Of F...mentioning

confidence: 99%

Fluorescent Aptasensors: Design Strategies and Applications in Analyzing Chemical Contamination of Food

et al. 2021

Anal. Chem.

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“…Aptamers are highly comparable to antibodies, although they show several advantages, such as cheap chemical fabrication, superior stability, and high reproducibility. Despite these unique properties, these molecules have been scarcely incorporated in MOF supports for sample treatment purposes due to their relatively short existence time [ 62 , 63 , 64 , 65 , 66 ]. Lin and co-workers [ 62 , 63 ] developed two aptamer-MOF devices for the selective extraction of polychlorinated biphenyls (PCBs) in soil and fish samples followed by GC-MS analysis.…”

Section: Applications Of Affinity-based Mof Materials In Sample Trmentioning

confidence: 99%

“…Additionally, aptamer-functionalized MOF materials have been applied for food safety purposes [ 65 , 66 ]. As an example, Zhang et al [ 65 ] synthesized a magnetic MOF (MIL-101) coupled with an aptamer for the enrichment of ochratoxin A in corn and peanut samples, and the determination was followed by UHPLC-MS-MS.…”

Section: Applications Of Affinity-based Mof Materials In Sample Trmentioning

confidence: 99%

Recent Advances in Affinity MOF-Based Sorbents with Sample Preparation Purposes

2020

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This review summarizes the recent advances concerning metal–organic frameworks (MOFs) modified with several biomolecules (e.g., amino acids, nucleobases, proteins, antibodies, aptamers, etc.) as ligands to prepare affinity-based sorbents for application in the sample preparation field. The preparation and incorporation strategies of these MOF-based affinity materials were described. Additionally, the different types of ligands that can be employed for the synthesis of these biocomposites and their application as sorbents for the selective extraction of molecules and clean-up of complex real samples is reported. The most important features of the developed biocomposites will be discussed throughout the text in different sections, and several examples will be also commented on in detail.

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Bimetallic organic framework-based aptamer sensors: a new platform for fluorescence detection of chloramphenicol

Cited by 22 publications

References 47 publications

A visual on-site biosensor for low-cost detection of chloramphenicol based on aptamer and split DNAzyme

A visual on-site biosensor for low-cost detection of chloramphenicol based on aptamer and split DNAzyme

Fluorescent Aptasensors: Design Strategies and Applications in Analyzing Chemical Contamination of Food

Recent Advances in Affinity MOF-Based Sorbents with Sample Preparation Purposes

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