Binary chromatographic fingerprinting for quality evaluation of <i>Radix Ophiopogonis</i> by high‐performance liquid chromatography coupled with ultraviolet and evaporative light‐scattering detectors

Liu, Li; Lu, Yun; Shao, Qing; Cheng, Yiyu; Qu, Haibin

doi:10.1002/jssc.200700214

Cited by 13 publications

(11 citation statements)

References 16 publications

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“…To avoid repeated analysis of some of the same compositions in PDA and ELSD [ 24 ] analyzed by chromatographic condition II, fourteen peaks were regarded as redundant peaks and excluded from the statistical calculations. Thus, 28 characteristic peaks marked as Y 1 to Y 28 in the PDA fingerprint analyzed by chromatographic condition II (HPLC-PDA), 9 characteristic peaks marked as Z 1 to Z 9 in the ELSD fingerprint analyzed by chromatographic condition II (HPLC-ELSD), and 19 characteristic peaks marked as X 1 to X 19 in the fingerprint analyzed by chromatographic condition I (HPLC-PDA) were selected to investigate the spectrum-effect relationships between the components and the pharmacological effect.…”

Section: Resultsmentioning

confidence: 99%

Multi-Dimensional Spectrum-Effect Relationship of the Impact of Chinese Herbal Formula Lichong Shengsui Yin on Ovarian Cancer

Wang

Li²,

Zhang

et al. 2017

Molecules

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Lichong Shengsui Yin (LCSSY) is an effective and classic compound prescription of Traditional Chinese Medicines (TCMs) used for the treatment of ovarian cancer. To investigate its pharmacodynamic basis for treating ovarian cancer, the multi-dimensional spectrum-effect relationship was determined. Four compositions (I to IV) were obtained by extracting LCSSY successively with supercritical CO2 fluid extraction, 75% ethanol reflux extraction, and the water extraction-ethanol precipitation method. Nine samples for pharmacological evaluation and fingerprint analysis were prepared by changing the content of the four compositions. The specific proportions of the four compositions were designed according to a four-factor, three-level L9(34) orthogonal test. The pharmacological evaluation included in vitro tumor inhibition experiments and the survival extension rate in tumor-bearing nude mice. The fingerprint analyzed by chromatographic condition I (high-performance liquid chromatography-photodiode array detec tor，HPLC-PDA) identified 19 common peaks. High-performance liquid chromatography-photodiode array detector-Evaporative Light-scattering Detector (HPLC-PDA-ELSD )hyphenated techniques were used to compensate for the use of a single detector, and the fingerprint analyzed by chromatographic condition II identified 28 common peaks in PDA and 23 common peaks in ELSD. Furthermore, multiple statistical analyses were utilized to calculate the relationships between the peaks and the pharmacological results. The union of the regression and the correlation analysis results were the peaks of X5, X9, X11, X12, X16, X18, Y5, Y8, Y12, Y14, Y20, Z4, Z5, Z6, and Z8. The intersection of the regression and the correlation analysis results were the peaks of X11, X12, X16, X18, Y5, Y12, and Z5. The correlated peaks were assigned by comparing the fingerprints with the negative control samples and reference standard samples, and identifying the structure using high-performance liquid chromatography-mass spectrometry detector(HPLC-MS). The results suggested that the pharmacodynamic basis of LCSSY on anti-ovarian cancer activities were germacrone, furandiene, β-elemene, calycosin-7-glucoside, ononin, epimedin B, icariin, ginsenoside Rc, astragaloside, ginsenoside Rd, astragaloside II, and some unknown components.

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Section: Resultsmentioning

confidence: 99%

Multi-Dimensional Spectrum-Effect Relationship of the Impact of Chinese Herbal Formula Lichong Shengsui Yin on Ovarian Cancer

Wang

Li²,

Zhang

et al. 2017

Molecules

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“…Unfortunately, research has mainly focused on homoisoflavanones because the reference standards are easy to obtain (Liu, Lu, Shao, Cheng, & Qu, 2007;Ye, Ye, Guo, & Huang, 2005). Few studies on the quantitative determination of steroidal saponins have been reported as its inherent Research, 2013Vol.…”

Section: Introductionmentioning

confidence: 98%

Determination of three steroidal saponins from Ophiopogon japonicus (Liliaceae) via high-performance liquid chromatography with mass spectrometry

Wang

2012

Natural Product Research

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A simple and accurate analytical method was developed for simultaneous quantification of three steroidal saponins in the roots of Ophiopogon japonicus via high-performance liquid chromatography (HPLC) with mass spectrometry (MS) in this study. Separation was performed on a Tigerkin C(18) column and detection was performed by mass spectrometry. A mobile phase consisted of 0.02% formic acid in water (v/v) and 0.02% formic acid in acetonitrile (v/v) was used with a flow rate of 0.5 mL min(-1). The quantitative HPLC-MS method was validated for linearity, precision, repeatability, stability, recovery, limits of detection and quantification. This developed method provides good linearity (r >0.9993), intra- and inter-day precisions (RSD <4.18%), repeatability (RSD <5.05%), stability (RSD <2.08%) and recovery (93.82-102.84%) for three steroidal saponins. It could be considered as a suitable quality control method for O. japonicus.

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“…Several studies have been conducted on the determination of Ophiopogonin D or Ophiopogonin D’ by ELSD,[ 18 19 ] total saponins by UV-VIS spectrophotometer,[ 8 20 21 ] homoisoflavonoids by HPLC,[ 22 ] diosgenin, and ruscogenin by nonaqueous capillary electrophoresis,[ 23 ] and fingerprint analysis have been used in some investigations. [ 17 24 ] However, until now few studies[ 25 ] focus on determination of ruscogenin in Ophiopogonis Radix by HPLC-ELSD.…”

Section: Introductionmentioning

confidence: 99%

Determination of ruscogenin in ophiopogonis radix by high-performance liquid chromatography-evaporative light scattering detector coupled with hierarchical clustering analysis

Liu

Feng

et al. 2016

Phcog Mag

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Background:Ophiopogonis Radix is a famous traditional Chinese medicine. It is necessary to establish a suitable quality control methods of Ophiopogonis Radix.Objective:To investigate the quality control methods of Ophiopogonis Radix by high-performance liquid chromatography (HPLC) coupled with evaporative light scattering detector (ELSD).Materials and Methods:A rapid and simple method, HPLC coupled with ELSD, was applied to determinate ruscogenin in 35 batches of Ophiopogenis Radix samples. Orthogonal tests and single factor explorations were used to optimize the extraction condition of ruscogenin. The content of ruscogenin in different origin was further analyzed by hierarchical clustering analysis (HCA).Results:The ruscogenin was successfully determined by HPLC-ELSD with a two-phase solvent system composed of methanol-water (88:12) at a flow rate 1.0 ml/min, column temperature maintained at 25°C, detector draft tube temperature at 42.2°C, nebulizer gas flow rate at 1.4 L/min, and the gain at 8. The result showed the good linearity of ruscogenin in the range of 40.20–804.00 μg/ml (R2 = 0.9996). Average of recovery was 101.3% (relative standard deviation = 1.59%). A significant difference of ruscogenin content was shown among 35 batches of Ophiopogenis Radix from different origin, varied from 0.0035% to 0.0240%. HCA based on the content of ruscogenin indicated that Ophiopogonis Radix in different origin was mainly divided into two clusters.Conclusion:This simple, rapid, low-cost, and reliable HPLC-ELSD method could be suitable for measurement of ruscogenin content rations and quality control of Ophiopogonis Radix.SUMMARY Ophiopogonis Radix is an important Traditional Chinese Medicine (TCM) to treat and prevent cardiovascular diseases and acute or chronic inflammation for thousands of years. Steroidal saponins were known as the dominant active components for their significant cardiovascular activity, and the most steroid sapogenin of them is ruscogenin. Therefore, ruscogenin was chosen as the marker component for evaluating the quality of Ophiopongonis Radix. This study focused on establishing a stable, low-cost, simple and practical method of HPLC-ELSD to determine the ruscogenin content, and 35 batches of samples of Ophiopogonis Radix were determined. Meanwhile, these results were analyzed by hierarchical clustering analysis and the methodology validation was based on USP34-NF-29 <1225>. Results showed that this analysis method was simple and stable, which would provide an important reference to establish the quality control methodology for other herb preparations and formulas containing Ophiopogonis Radix.

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Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high‐performance liquid chromatography coupled with ultraviolet and evaporative light‐scattering detectors

Cited by 13 publications

References 16 publications

Multi-Dimensional Spectrum-Effect Relationship of the Impact of Chinese Herbal Formula Lichong Shengsui Yin on Ovarian Cancer

Multi-Dimensional Spectrum-Effect Relationship of the Impact of Chinese Herbal Formula Lichong Shengsui Yin on Ovarian Cancer

Determination of three steroidal saponins from Ophiopogon japonicus (Liliaceae) via high-performance liquid chromatography with mass spectrometry

Determination of ruscogenin in ophiopogonis radix by high-performance liquid chromatography-evaporative light scattering detector coupled with hierarchical clustering analysis

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