Binding efficiency of recombinant collagen‐binding basic fibroblast growth factors (CBD‐bFGFs) and their promotion for NIH‐3T3 cell proliferation

Wu, Zhenxu; Zhou, Yulai; Chen, Li; Hu, Mingxin; Wang, Yu; Li, Linlong; Wang, Zongliang; Zhang, Peibiao

doi:10.1002/bip.23105

Cited by 6 publications

(4 citation statements)

References 44 publications

(86 reference statements)

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“…Frontiers in Bioengineering and Biotechnology frontiersin.org 12 Duan et al 10.3389/fbioe.2023.1258666 collagen materials, while the biological activity of CBD-bFGF was not affected (Zhao et al, 2007;Wu et al, 2018). Dai and his colleagues first introduced von Willeband's factor (vWF)-derived WREPSFCALS and collagenase-derived TKKTLRT amino acid residues into natural bFGF to recombine two fusion proteins, V-bFGF and C-bFGF, respectively, for targeted therapy of wound repair with less dosage of growth factors (Desouza and Brentani, 1992;Nishi et al, 1998;Andrades et al, 2001;Zhao et al, 2007).…”

Section: Growth Factorsmentioning

confidence: 99%

Bioactive Materials for Disease Diagnosis and Therapy

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Section: Growth Factorsmentioning

confidence: 99%

Bioactive Materials for Disease Diagnosis and Therapy

2024

Frontiers Research Topics

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“…To overcome the shortcomings of short half-life and fast diffusion of bFGF in medical applications and improve its binding efficiency with biomaterials, researchers engineered a bFGF fusion protein containing collagen-binding domain (CBD-bFGF) using gene recombination technology. And thus due to the introduction of CBD motifs, bFGF acquired significant binding capacity with collagen materials, while the biological activity of CBD-bFGF was not affected ( Zhao et al, 2007 ; Wu et al, 2018 ). Dai and his colleagues first introduced von Willeband’s factor (vWF)-derived WREPSFCALS and collagenase-derived TKKTLRT amino acid residues into natural bFGF to recombine two fusion proteins, V-bFGF and C-bFGF, respectively, for targeted therapy of wound repair with less dosage of growth factors ( Desouza and Brentani, 1992 ; Nishi et al, 1998 ; Andrades et al, 2001 ; Zhao et al, 2007 ).…”

Section: Biological Functionalization Strategiesmentioning

confidence: 99%

Research progress of biomaterials and innovative technologies in urinary tissue engineering

Duan

Wang

Fan

et al. 2023

Front. Bioeng. Biotechnol.

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Substantial interests have been attracted to multiple bioactive and biomimetic biomaterials in recent decades because of their ability in presenting a structural and functional reconstruction of urinary tissues. Some innovative technologies have also been surging in urinary tissue engineering and urological regeneration by providing insights into the physiological behavior of the urinary system. As such, the hierarchical structure and tissue function of the bladder, urethra, and ureter can be reproduced similarly to the native urinary tissues. This review aims to summarize recent advances in functional biomaterials and biomimetic technologies toward urological reconstruction. Various nanofirous biomaterials derived from decellularized natural tissues, synthetic biopolymers, and hybrid scaffolds were developed with desired microstructure, surface chemistry, and mechanical properties. Some growth factors, drugs, as well as inorganic nanomaterials were also utilized to enhance the biological activity and functionality of scaffolds. Notably, it is emphasized that advanced approaches, such as 3D (bio) printing and organoids, have also been developed to facilitate structural and functional regeneration of the urological system. So in this review, we discussed the fabrication strategies, physiochemical properties, and biofunctional modification of regenerative biomaterials and their potential clinical application of fast-evolving technologies. In addition, future prospective and commercial products are further proposed and discussed.

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“…Another advantage of this kind of growth factor is that the biological activity of the growth factor cannot be destroyed but also makes the polymer materials perform long-term tissue repair. For example, some studies prepared recombinant bFGF with collagen-anchoring ability to enhance the binding of bFGF and collagen scaffold, and the result indicated that this recombinant bFGF can obviously promote cell proliferation compared with commercial bFGF ( Wu et al, 2018 ). ( Zhang et al, 2019 ) introduced the DOPA domain into growth factor (IGF-1) via recombinant DNA technology and found that recombinant IGF-1 can be tightly bound to the surface of PLGA film ( Zhang et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Acceleration of Healing in Full-Thickness Wound by Chitosan-Binding bFGF and Antimicrobial Peptide Modification Chitosan Membrane

Hou

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Skin wound healing is an important clinical challenge, and the main treatment points are accelerating epidermal regeneration and preventing infection. Therefore, it is necessary to develop a wound dressing that can simultaneously cure bacterial infections and accelerate wound healing. Here, we report a multifunctional composite wound dressing loaded with chitosan (CS)-binding bFGF (CSBD-bFGF) and antimicrobial peptides (P5S9K). First, CS was used as the dressing matrix material, and P5S9K was encapsulated in CS. Then, CSBD-bFGF was designed by combining recombinant DNA technology and tyrosinase treatment and modified on the dressing material surface. The results show that the binding ability of CSBD-bFGF and CS was significantly improved compared with that of commercial bFGF, and CSBD-bFGF could be controllably released from the CS dressing. More importantly, the prepared dressing material showed excellent antibacterial activity in vivo and in vitro and could effectively inhibit the growth of E. coli and S. aureus. Using NIH3T3 cells as cellular models, the results showed that the CSBD-bFGF@CS/P5S9K composite dressing was a friendly material for cell growth. After cells were seeded on the composite dressing surface, collagen-1 (COL-1) and vascular endothelial growth factor (VEGF) genes expression in cells were significantly upregulated. Finally, the full-thickness wound of the rat dorsal model was applied to analyse the tissue repair ability of the composite dressing. The results showed that the composite dressing containing CSBD-bFGF and P5S9K had the strongest ability to repair skin wounds. Therefore, the CSBD-bFGF@CS/P5S9K composite dressing has good antibacterial and accelerated wound healing abilities and has good application prospects in the treatment of skin wounds.

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Binding efficiency of recombinant collagen‐binding basic fibroblast growth factors (CBD‐bFGFs) and their promotion for NIH‐3T3 cell proliferation

Cited by 6 publications

References 44 publications

Bioactive Materials for Disease Diagnosis and Therapy

Bioactive Materials for Disease Diagnosis and Therapy

Research progress of biomaterials and innovative technologies in urinary tissue engineering

Acceleration of Healing in Full-Thickness Wound by Chitosan-Binding bFGF and Antimicrobial Peptide Modification Chitosan Membrane

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