Binding of Adenosine Diphosphate by Human Platelet Membrane

Nachman, Ralph L.

doi:10.1002/9780470720172.ch3

Cited by 3 publications

(1 citation statement)

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“…The platelet contractile proteins are one of the more thoroughly characterized non‐muscle contractile systems and may be central to platelet hemostatic function [Pollard, ], including the granules content release [Nachman and Ferris, ], aggregation [Puszkin et al, ], and clot retraction [Pollard, ]; focal adhesions also referred to as focal contacts are essential sensors of the platelet tension generated during the adhesion process [Hagmann, ].…”

Section: Discussionmentioning

confidence: 99%

Association of Membrane/Lipid Rafts With the Platelet Cytoskeleton and the Caveolin PY14: Participation in the Adhesion Process

Cerecedo

Martínez‐Vieyra

Maldonado‐García

et al. 2015

J of Cellular Biochemistry

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Platelets are the most prominent elements of blood tissue involved in hemostasis at sites of blood vessel injury. Platelet cytoskeleton is responsible for their shape modifications observed during activation and adhesion to the substratum; therefore the interactions between cytoskeleton and plasma membrane are critical to modulate blood platelet functions. Several cytoskeletal components and binding partners, as well as enzymes that regulate the cytoskeleton, localize to membrane/lipid rafts (MLR) and regulate lateral diffusion of membrane proteins and lipids. Resting, thrombin-activated, and adherent human platelets were processed for biochemical studies including western-blot and immunprecipitation assays and confocal analysis were performed to characterize the interaction of MLR with the main cytoskeleton elements and β-dystroglycan as well as with the association of caveolin-1 PY14 with focal adhesion proteins. We transfected a megakaryoblast cell line (Meg-01) to deplete β-dystroglycan, subsequent to their differentiation to the platelet progenitors. Our data showed a direct interaction of the MLR with cytoskeleton to regulate platelet shape, while an association of caveolin-1 PY14 with vinculin is needed to establish focal adhesions, which are modulated for β-dystroglycan. In conclusion, caveolin-1 PY14 in association with platelet cytoskeleton participate in focal adhesions dynamics.

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Section: Discussionmentioning

confidence: 99%