Binding of Streptococcus gordonii to oral epithelial monolayers increases paracellular barrier function

Harty, D. W. S.; Commandeur, Zoe; Hunter, Neil

doi:10.1016/j.micpath.2012.11.004

Cited by 21 publications

(23 citation statements)

References 43 publications

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“…In our experimental model, the release of IL-6 and PGE 2 was not in line with that seen in previous studies that demonstrated an inflammatory response in HGFs exposed to different resin composites [17], [31]. HGFs are an important regulatory cell-type in the progression of periodontitis.…”

Section: Discussionsupporting

confidence: 60%

“…The human oral cavity is colonized by a crowd of microorganisms of which streptococci are the most plentiful. Commensal bacterial species can form tight associations with host epithelial tissue, benefitting oral health [16], [17]. Streptococcus mitis , a commensal microorganism, that is part of the oral flora, colonizes hard surfaces in the oral cavity such as dental hard tissues, as well as mucous membranes.…”

Section: Introductionmentioning

confidence: 99%

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Biological Responses of Human Gingival Fibroblasts (HGFs) in an Innovative Co-Culture Model with Streptococcus mitis to Thermosets Coated with a Silver Polysaccharide Antimicrobial System

et al. 2014

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This study sought to evaluate the in vitro biological response of human gingival fibroblasts (HGFs) co-coltured with Streptococcus mitis to bisphenol A glycidylmethacrylate/triethylene glycol dimethacrylate (BisGMA/TEGDMA) thermosets coated with Chitlac-nAg, a nanocomposite system with antimicrobial properties. To avoid bacterial adhesion to dental devices and to reduce cytotoxicity against eukaryotic cells, we coated BisGMA/TEGDMA methacrylic thermosets with a new material, Chitlac-nAg, formed by stabilizing silver nanoparticles, which have well-known antimicrobial properties, with a polyelectrolyte solution containing Chitlac. Cytotoxicity, cell morphology, cell migration and inflammatory interleukine-6 (IL-6) and prostaglandin E2 (PGE2) secretion were evaluated. Our results showed that the cytotoxicity exerted on HGFs by our nanocomposite material was absent in our co-culture model, where fibroblasts are able to adhere and migrate. After 24 h thermosets coated with Chitlac as well as those coated with Chitlac-nAg exerted a minimal cytotoxic effect on HGFs, while after 48 h LDH release rises up 20%. Moreover the presence of S. mitis reduced this release in a greater amount with Chitlac-nAg coated thermosets. The secretion of IL-6 was significant in both Chitlac and Chitlac-nAg coated thermosets, but PGE2 production was minimal, suggesting that the IL-6 production was not related to an inflammatory response. Co-culture and the addiction of saliva did not influence IL-6 and PGE2 secretion. Data obtained in the present work suggest that Chitlac n-Ag coated thermosets could significantly improve the success rates of restorative dentistry, since they limit bacterial adhesion and are not toxic to HGFs.

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Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Biological Responses of Human Gingival Fibroblasts (HGFs) in an Innovative Co-Culture Model with Streptococcus mitis to Thermosets Coated with a Silver Polysaccharide Antimicrobial System

et al. 2014

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“…All confocal images were captured with an Olympus Fluoview (FV) 1000, equipped with Olympus FV 10-MCPSU (405, 473, 633 nm) and NTT Electronic Optiλ (559-nm) lasers [Ye et al, 2013]. Fields were selected at random (objective: Olympus 40X/1.30/0.20 [WD] Oil UPLFLN) and the views were brought into focus under bright-field conditions [Ye et al, 2013]. Ten random regions of interest in the odontoblastic layer ( fig.…”

Section: Analysis Of Distribution Pattern and Density Of Gfap S100bmentioning

confidence: 99%

“…1 a, stage C). Fluorescence images were captured using confocal acquisition software (FV10-ASW 1.7), stored and exported as TIF files [Ye et al, 2013] and analysed using Image J software (version 1.410) (details of analysis in online suppl. methods and online suppl.…”

Section: Analysis Of Distribution Pattern and Density Of Gfap S100bmentioning

confidence: 99%

Glial Network Responses to Polymicrobial Invasion of Dentin

Houshmandi

Hunter

2014

Caries Res

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This study investigated the distribution patterns of glial networks disclosed by reactivity for glial fibrillary acidic protein (GFAP) and S100B in healthy and carious human teeth. The objective was to determine the assembly and collapse of glial networks in response to encroaching infection. 15 healthy and 37 carious posterior teeth from adults were studied. Immediately after extraction, teeth were cleaned and vertically split and the half with pulp fixed and prepared for resin or frozen sections. Sections were stained with toluidine blue and for immunofluorescence, with observation by confocal laser microscopy and analysis by ImageJ software. Carious teeth were subdivided into three groups according to degree of carious involvement: microbial penetration through enamel (stage A), extension into dentin (stage B) and advanced penetration into dentin but without invasion of underlying pulp tissue (stage C). In stage A lesions there was marked increase in glial networks in dental pulp tissue that extended beyond the zone of microbial invasion. This response was maintained in stage B lesions. In advanced stage C lesions these networks were degraded in the zone of invasion in association with failure to contain infection. Cells expressing the glial markers GFAP and S100B showed a response to initial microbial invasion of dentin by increase in number and altered anatomical arrangement. The late stage of dentinal caries was marked by collapse of these networks in the region adjacent to advancing bacteria. This behaviour is important for understanding and explaining the defensive response of the neurosensory peripheral dental pulp apparatus to infection.

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“…For drug development assay, oral pathogen P.gingivalis strain ATCC 33277 at MOI (multiplicity of infection) 100 cells per one epithelial cell [11] was added to confluent H413 clone-1 epithelial monolayers for 1.5 h at 37 °C in 5 % CO 2 . Monolayers were washed twice with Dulbecco’s phosphate-buffered saline (DPBS) [12].…”

Section: Methodsmentioning

confidence: 99%

Three/four-dimensional (3D/4D) microscopic imaging and processing in clinical dental research

Houshmandi

2016

BMC Oral Health

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Background: Confocal laser scanning microscope (CLSM) has been widely employed in our laboratory for structural and functional analysis of clinical dental specimens and live cell imaging of cultured oral epithelial cells. Methods: In this vitro study, a Fluoview 1000 (Olympus) confocal system was utilised to study thick sections of carious lesions (40-100 μm) and periodontal disease tissue samples (20-40 μm) by 2D Z stacking imaging and 3-dimentional (3D) reconstruction. Four-dimensional (4D) imaging when including time or position points was used for live cells to assess penetration/localisation/co-localization of oral pathogen proteins and therapeutic drugs.

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Binding of Streptococcus gordonii to oral epithelial monolayers increases paracellular barrier function

Cited by 21 publications

References 43 publications

Biological Responses of Human Gingival Fibroblasts (HGFs) in an Innovative Co-Culture Model with Streptococcus mitis to Thermosets Coated with a Silver Polysaccharide Antimicrobial System

Biological Responses of Human Gingival Fibroblasts (HGFs) in an Innovative Co-Culture Model with Streptococcus mitis to Thermosets Coated with a Silver Polysaccharide Antimicrobial System

Glial Network Responses to Polymicrobial Invasion of Dentin

Three/four-dimensional (3D/4D) microscopic imaging and processing in clinical dental research

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