Binucleated and micronucleated blastomeres in embryos derived from human assisted reproduction cycles

Meriano, Jim; Clark, Christine A.; Cadesky, Ken; Laskin, Carl A.

doi:10.1016/s1472-6483(10)61635-5

Cited by 85 publications

(59 citation statements)

References 33 publications

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“…Detection can be achieved by time studies [5], but these might compromise embryo quality. Conciliating the number and timing of embryo visualizations whilst avoiding disturbing them is critical.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of multinucleated (MN) blastomeres in embryos arising through IVF procedures has been described to range from 14.3% to 74% [1][2][3][4][5][6] and 43% to 87% of the patients to have MN embryos [3,4,7].…”

Section: Introductionmentioning

confidence: 99%

“…Multinucleation has been associated with developmental arrest [1-4, 7, 12-15], poor embryo morphology [1,2,7], elevated fragmentation [2,3], repeated cycles [3], blastomere asymmetry [16,17] and higher multinucleation rate on 2-cells when compared with more developed embryos [1,3,5,7,13,15,18]. Multinucleation has also been associated with higher blastomere diameter [1,6], decrease in blastocyst formation rate [5,7,15,19], reduced capacity of development on siblings of MN embryos [2,5], high amount of oocytes retrieved and elevated E 2 levels [2,3,20], follicles hypoxia [21], culture media [13,22], the use of GnRH antagonist [23] and apoptosis [6].…”

Section: Introductionmentioning

confidence: 99%

“…Multinucleation has also been associated with higher blastomere diameter [1,6], decrease in blastocyst formation rate [5,7,15,19], reduced capacity of development on siblings of MN embryos [2,5], high amount of oocytes retrieved and elevated E 2 levels [2,3,20], follicles hypoxia [21], culture media [13,22], the use of GnRH antagonist [23] and apoptosis [6].…”

Section: Introductionmentioning

confidence: 99%

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Insights on blastomere nuclearity

Gil

Gustavo

Póo

et al. 2006

J Assist Reprod Genet

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Purpose: To analyze the results of our transferred embryos, especially those that "changed" their blastomere nuclearity from Multinucleated (MN) to Mono-nucleated during development.Methods: Pregnancies where at least one MN embryo was transferred were retrospectively evaluated and categorized in order to record and follow-up on the ones that were implanted. Embryos were classified as normal (when all blastomeres were mono-nucleated on day one and two of development), corrected (multinucleated embryos on day one that became mono-nucleated on day two) and non-corrected (multinucleated either on day one, on day two or both days).Results: There were 633 transfer cycles analyzed. Thirtythree percent (206) had at least one embryo with a MN blastomere at a given stage of development. Pregnancy and implantation rates were 29.0% and 19.0% for the group of exclusively mono-nucleated embryo transfers, and 28.6% and 15.8% for the group with at least one MN embryo transferred. The pregnancy outcome for "corrected" and "noncorrected" embryos could be corroborated unequivocally in only 9 cases, with an outcome of 8 and 4 normal babies, respectively.Conclusions: Because the amount of data analyzed is not satisfactorily large, differences were not significantly different; however, a trend may exist showing that normal at term pregnancies obtained from corrected embryos are more likely to occur than those from non-corrected embryos. Nuclear observation on a daily basis should be one of the strate-Clínica de Fertilidad, Centro Médico Docente La Trinidad, Caracas, Venezuela e-mail: mgilco@cantv.net gies used to select the best embryos for transferring, to improve implantation rates and avoid multiple pregnancies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Insights on blastomere nuclearity

Gil

Gustavo

Póo

et al. 2006

J Assist Reprod Genet

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“…Other abnormalities include multi-or micronucleated blastomeres [4,5]. Bi-and multinucleated blastomeres in general are more frequent in embryos with poor morphology [6] and quality and development [7] and are associated with lower pregnancy rates [8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos

Agerholm¹,

Hnida

Crüger

et al. 2008

J Assist Reprod Genet

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Purpose The aim was to elucidate if the nuclear size and number are indicative of aberrant chromosome content in human blastomeres and embryos. Methods The number of nuclei and the nucleus and blastomere size were measured by a computer controlled system for multilevel analysis. Then the nuclei were enumerated for 13 chromosomes by a combination of PNA and DNA probes. Results In the mononucleated embryos there was no difference in the mean size of chromosomally normal and abnormal nuclei but a significant difference in the mean nuclei size of nuclei that had gained chromosomes compared to nuclei that had lost chromosomes. The nuclei from multinucleated blastomeres had a significant smaller mean size and the frequency of chromosomally aberrant blastomeres was significantly higher. Conclusion The mean nuclear size is not a marker for the chromosome content in mononucleated embryos. However, it seems that the nuclei size can be related to multinucleation and maybe to the chromosome content.

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Morphological Assessment of Embryos in Culture

Critchlow¹

2018

Clinical Reproductive Science

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Binucleated and micronucleated blastomeres in embryos derived from human assisted reproduction cycles

Cited by 85 publications

References 33 publications

Insights on blastomere nuclearity

Insights on blastomere nuclearity

Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos

Morphological Assessment of Embryos in Culture

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