Bio-click chemistry: a bridge between biocatalysis and click chemistry

proyectos, Serrano Rodríguez, Daniel, especialista de; Moglie, Yanina; Ramírez‐Sarmiento, César A.; Singh, Sachin Kumar; Dua, Kamal; Zacconi, Flavia

doi:10.1039/d1ra08053a

Cited by 12 publications

(5 citation statements)

References 116 publications

(138 reference statements)

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“…The concept of “click biology”—by analogy with the more widely known term “click chemistry”—represents the convenience obtained by effortlessly attaching two moieties through bio-molecular interactions. It often refers to biotransformation processes, DNA ligation, or formation of highly ordered structures via hybridization [ 22 ]. Preferably, each bioreceptor molecule is expected to be linked to one molecule of anchor DNA, and the conjugates should be separated from the unbound components.…”

Section: Resultsmentioning

confidence: 99%

A Careful Insight into DDI-Type Receptor Layers on the Way to Improvement of Click-Biology-Based Immunosensors

Karoń,

Drozd,

Malinowska

2024

Biosensors

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Protein-based microarrays are important tools for high-throughput medical diagnostics, offering versatile platforms for multiplex immunodetection. However, challenges arise in protein microarrays due to the heterogeneous nature of proteins and, thus, differences in their immobilization conditions. This article advocates DNA-directed immobilization (DDI) as a solution, emphasizing its rapid and cost-effective fabrication of biosensing platforms. Thiolated single-stranded DNA and its analogues, such as ZNA® and PNA probes, were used to immobilize model proteins (anti-CRP antibodies and SARS-CoV nucleoprotein). The study explores factors influencing DDI-based immunosensor performance, including the purity of protein-DNA conjugates and the stability of their duplexes with DNA and analogues. It also provides insight into backfilling agent type and probe surface density. The research reveals that single-component monolayers lack protection against protein adsorption, while mixing the probes with long-chain ligands may hinder DNA-protein conjugate anchoring. Conventional DNA probes offer slightly higher surface density, while ZNA® probes exhibit better binding efficiency. Despite no enhanced stability in different ionic strength media, the cost-effectiveness of DNA probes led to their preference. The findings contribute to advancing microarray technology, paving the way for new generations of DDI-based multiplex platforms for rapid and robust diagnostics.

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Section: Resultsmentioning

confidence: 99%

A Careful Insight into DDI-Type Receptor Layers on the Way to Improvement of Click-Biology-Based Immunosensors

Karoń,

Drozd,

Malinowska

2024

Biosensors

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“…The widespread use of straightforward click reactions in biological environments led to the advent of new definition “bio-click chemistry” which can be understood toward reactions of functional groups commonly found in biological molecules like proteins or live cells and by exploiting the potential of click reactions (Rodríguez et al, 2022). There is a close overlap between click and bioorthogonal chemistry.…”

Section: Bioorthogonal Click Chemistrymentioning

confidence: 99%

Protein click chemistry and its potential for medical applications

Amiri,

Abedanzadeh,

Davaeil

et al. 2024

Quart. Rev. Biophys.

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A revolution in chemical biology occurred with the introduction of click chemistry. Click chemistry plays an important role in protein chemistry modifications, providing specific, sensitive, rapid, and easy-to-handle methods. Under physiological conditions, click chemistry often overlaps with bioorthogonal chemistry, defined as reactions that occur rapidly and selectively without interfering with biological processes. Click chemistry is used for the posttranslational modification of proteins based on covalent bond formations. With the contribution of click reactions, selective modification of proteins would be developed, representing an alternative to other technologies in preparing new proteins or enzymes for studying specific protein functions in different biological processes. Click-modified proteins have potential in diverse applications such as imaging, labeling, sensing, drug design, and enzyme technology. Due to the promising role of proteins in disease diagnosis and therapy, this review aims to highlight the growing applications of click strategies in protein chemistry over the last two decades, with a special emphasis on medicinal applications.

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“…Here, the temptation is great to look for a portmanteau word, and thus the term "bio-click" (hyphenated or not) has been sporadically used, in particular by Anseth and coworkers. 17 However, this neologism has also been proposed in the field of biocatalysis to describe new chemoenzymatic reactions combining biocatalysts and click chemistry, 18 which could bring confusion. More importantly, one may expect that a "bioclick" reaction describes a click reaction that allows for grafting onto specific biological compounds; and the orthogonal character of the reaction is definitely getting lost along the way.…”

Section: The Tricky Case Of the Dynamic Covalent Reactionsmentioning

confidence: 99%

Rethinking Click and Bioorthogonal Chemistry for Biomedical Applications

Delplace

2023

ACS Materials Lett.

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Over the last two decades, click chemistry and bioorthogonal chemistry have revolutionized the field of biomedical engineering. Extensive investigations have been conducted and allowed for unforeseeable developments in many different fields, including imaging, drug delivery, 3D cell culture, and bioprinting. The properties of advanced click and bioorthogonal chemical tools have now exceeded the expectations set by the researchers who first introduced the two concepts, calling for reevaluation. More importantly, their developments in distinct research contexts have led to conceptual divergences, which can now be a source of misunderstandings and disagreements. This perspective article aims to take a fresh look at the two concepts, with particular attention to their combination for applications in biological environments. After a short historical introduction, the definitions of click chemistry and bioorthogonal chemistry are revised, and the concept of ideal click chemistry is introduced. Chemical reactions that are commonly considered as click and bioorthogonal reactions are discussed in light of this new perspective. Finally, current challenges and new chemical concepts are presented, offering a new framework for future research.

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Bio-click chemistry: a bridge between biocatalysis and click chemistry

Abstract: This review summarizes chemo/biocatalytic syntheses that combine the highly efficient click chemistry reactions with the advantages of biocatalysis. The concept of bio-click chemistry and its implications are introduced in this review.

Cited by 12 publications

References 116 publications

A Careful Insight into DDI-Type Receptor Layers on the Way to Improvement of Click-Biology-Based Immunosensors

A Careful Insight into DDI-Type Receptor Layers on the Way to Improvement of Click-Biology-Based Immunosensors

Protein click chemistry and its potential for medical applications

Rethinking Click and Bioorthogonal Chemistry for Biomedical Applications

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