“…31 For pests, Ag 0 NMs (35 nm, 150 mg L −1 ) prepared using Dicrocephala integrifolia extract as a reducing agent exhibited antifeedant activity against Spodoptera litura (76.9%) and Helivoverpa armigera (79.2%), and the antifeedant activity was statistically greater than that associated with the equivalent AgNO 3 , exhibiting a significant nano-effect. 32 Ag 0 NMs at 200 mg kg −1 caused 52.0% mortality of pathogenic nematodes after 3 days exposure, and the application of Ag 0 NMs showed no effect on the growth of plant and the non-pathogenic nematode. 33 Ag 0 NMs showed great efficacy for the control of crop disease and pest in the study by Ahmed et al (2020), however, the biotransformation and toxicity to non-target organisms of Ag 0 NMs in the environment is still a major concern and the life cycle analysis of Ag 0 NMs is missing.…”