Bio-functionalized Gold Nanoparticles: A Potent Probe for Profound Antibacterial Efficiency through Drug Delivery System

Singh, Manoj; Manikandan, S.; Yadav, Mukesh; Kumar, S. Himagirish; Sehrawat, Nirmala; Meashi, Vikas; Diksha, .; Sharma, Pranjal; Sharma, Anil Kumar

doi:10.5530/ajbls.2020.9.21

Cited by 5 publications

(5 citation statements)

References 12 publications

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“…Microbial assays were utilized to compare the suppression of sensitive bacteria growth generated by known quantities of the test antibiotic and a control drug. E. coli and S. aureus, two commonly used gram-negative and gram-positive bacteria, were grown in nutritional broth overnight at 37 °C for 24 h. A spectrophotometer was used to quantify the optical density at 620 nm and analyse bacterial growth curves [41].…”

Section: Bacterial Culturementioning

confidence: 99%

“…The antibiotic-coated IO-NPs were then put on agar plates and incubated for at least an hour at 25 °C to allow for pre-incubation diffusion, which reduced the effect of timing variations when various solutions were utilized. The plates were examined for antibacterial activity after 12-24 h of incubation at 37 °C by measuring the breadth of the inhibition zones for each bacterial culture [41].…”

Section: Microbial Assaymentioning

confidence: 99%

“…Antibacterial activity was measured by increasing fold area and given as the diameter of the zone of inhibition. Experiments were carried out in triplicate, and the average inhibitory zone diameter of pure antibiotic and antibiotic coated-IO-NPs, as well as the standard deviation, were calculated [41].…”

Section: Assessment Of Antimicrobial Activitymentioning

confidence: 99%

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Green synthesis, characterization and drug-loaded iron oxide nanoparticles derived from Nerium oleander flower extract as a nanocarrier for in vitro antibacterial efficacy

Sharma,

Kansay

et al. 2024

Nano Ex.

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Application of drug conjugated iron oxide hematite (α-Fe2O3) nanoparticles are of tremendous interest in biomedicine nowadays. Meanwhile, green production of iron oxide nanoparticles is gaining favour due to its sustainability, ease of usage, and biocompatibility. Therefore, this work reports on the use of hexahydrate ferric chloride and nerium oleander flower extract to synthesize nanoscaled hematite (α-Fe2O3) iron oxide particles conjugated with various drugs for antibacterial agents. Diverse morphological, physicochemical, structural, optical, and magnetic characteristics have been characterized using FESEM, EDX, XRD, UV–vis, FTIR, Raman and vibrating sample magnetometer. The synthesis of the polyshaped iron oxide nanoparticles, with average sizes ranging from 47.2 ± 20 nm, was accomplished. Furthermore, temperature-dependent variations in magnetic behavior were observed during calcination. The XRD and Raman spectra revealed hematite (α-Fe2O3) type formation of iron oxide nanoparticles. Only calcinated IO-NPs at high temperatures (700 °C) demonstrated low coercivity and residual magnetism, which revealed weak ferromagnetic ordering; other calcinated samples, including nascent ones, showed incredibly weak ferromagnetic ordering. Besides, the effectiveness of drug-encapsulated iron oxide nanoparticles against bacteria in vitro was examined. It was interesting to observe that gentamycin-coated IO-NPs tended to be more susceptible to S. aureus than E. coli bacteria, but streptomycin-conjugated IO-NPs showed the reverse trend. However, as compared to the nascent sample and the high temperature (700 °C) calcinated sample, both antibiotic-loaded IO-NPs displayed better inhibitory abilities.

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Section: Bacterial Culturementioning

confidence: 99%

Section: Microbial Assaymentioning

confidence: 99%

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Green synthesis, characterization and drug-loaded iron oxide nanoparticles derived from Nerium oleander flower extract as a nanocarrier for in vitro antibacterial efficacy

Sharma,

Kansay

et al. 2024

Nano Ex.

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“…In addition, these nanocarriers are nontoxic, biodegradable, biocompatible and efficiently removed from the human body through iron metabolism. Several drugs have been combined with iron oxide nanoparticles to improve its antibacterial properties [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Chloramphenicol and Gentamycin-encapsulated Iron Oxide Nanoparticles as a Nanocarrier for Antibacterial Efficacy via Targeted Drug Delivery

Sharma,

Kansay

et al. 2023

Nano Biomedicine and Engineering

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Surface functionalization of iron oxide nanoparticles (Fe 2 O 3 NPs) with antibiotics is a novel approach that opens the door to drug delivery applications. In the present work, we report iron oxide nanoparticles synthesized by chemical co-precipitation method. As-synthesized nanoparticles were characterized using field emission scanning electron microscopy (FESEM) , energy dispersive X-ray (EDX), X-ray diffraction (XRD), ultraviolet (UV)-visible (Vis) spectroscopy, Fourier transform infrared (FTIR), and vibrating sample magnetometer (VSM). The poly-shaped Fe 2 O 3 NPs of size (34 ± 10) nm with hematite (α-Fe 2 O 3 ) phase were synthesized. The antibacterial activity of chloramphenicol and gentamicin and their formulation with encapsulated iron oxide nanoparticles was investigated by the agar well diffusion technique. Drug-encapsulated Fe 2 O 3 NPs showed antibacterial activity against Escherichia coli and Staphylococcus aureus strains, possibly in a dose-dependent manner. Significant effectiveness was confirmed by the increase in the single range of inhibition against the tested microorganisms. Furthermore, the effect of iron oxide nanoparticle concentrations ranging from 1 to 9 μg/μL on bacterial growth was examined.

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“…The presence of biomolecules adsorbed on the NPs surface makes them safer for medical applications as therapeutic tools or antibacterial agents [45,46]. In the latter case, the capping agents were able to bind receptors of bacterial membranes damaging the respiratory system causing cell death [47,48]. Among different types of Mediterranean plants, Laurus Nobilis [49] is a valid source of polar molecules, such as polyphenols, which are demonstrated to have strong antioxidant and anti-inflammatory effects [50,51].…”

Section: Introductionmentioning

confidence: 99%

Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

et al. 2022

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The use of silver nanoparticles (Ag NPs) in the biomedical field deserves a mindful analysis of the possible inflammatory response which could limit their use in the clinic. Despite the anti-cancer properties of Ag NPs having been widely demonstrated, there are still few studies concerning their involvement in the activation of specific inflammatory pathways. The inflammatory outcome depends on the synthetic route used in the NPs production, in which toxic reagents are employed. In this work, we compared two types of Ag NPs, obtained by two different chemical routes: conventional synthesis using sodium citrate and a green protocol based on leaf extracts as a source of reduction and capping agents. A careful physicochemical characterization was carried out showing spherical and stable Ag NPs with an average size between 20 nm and 35 nm for conventional and green Ag NPs respectively. Then, we evaluated their ability to induce the activation of inflammation in Human Leukemic Monocytes (THP-1) differentiated into M0 macrophages using 1 µM and 2 µM NPs concentrations (corresponded to 0.1 µg/mL and 0.2 µg/mL respectively) and two-time points (24 h and 48 h). Our results showed a clear difference in Nuclear Factor κB (NF-κb) activation, Interleukins 6–8 (IL-6, IL-8) secretion, Tumor Necrosis Factor-α (TNF-α) and Cyclooxygenase-2 (COX-2) expression exerted by the two kinds of Ag NPs. Green Ag NPs were definitely tolerated by macrophages compared to conventional Ag NPs which induced the activation of all the factors mentioned above. Subsequently, the exposure of breast cancer cell line (MCF-7) to the green Ag NPs showed that they exhibited antitumor activity like the conventional ones, but surprisingly, using the MCF-10A line (not tumoral breast cells) the green Ag NPs did not cause a significant decrease in cell viability.

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Bio-functionalized Gold Nanoparticles: A Potent Probe for Profound Antibacterial Efficiency through Drug Delivery System

Cited by 5 publications

References 12 publications

Green synthesis, characterization and drug-loaded iron oxide nanoparticles derived from Nerium oleander flower extract as a nanocarrier for in vitro antibacterial efficacy

Green synthesis, characterization and drug-loaded iron oxide nanoparticles derived from Nerium oleander flower extract as a nanocarrier for in vitro antibacterial efficacy

Chloramphenicol and Gentamycin-encapsulated Iron Oxide Nanoparticles as a Nanocarrier for Antibacterial Efficacy via Targeted Drug Delivery

Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

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