Abstract-It is well known that biology-inspired selfmaintaining algorithms in wireless sensor nodes achieve near optimum time division multiple access (TDMA) characteristics in a decentralized manner and with very low complexity. We extend such distributed TDMA approaches to multiple channels (frequencies). This is achieved by extending the concept of collaborative reactive listening in order to balance the number of nodes in all available channels. We prove the stability of the new protocol and estimate the delay until the balanced system state is reached. Our approach is benchmarked against singlechannel distributed TDMA and channel hopping approaches using TinyOS imote2 wireless sensors.Index Terms-biology inspired desynchronization, multi-channel MAC, TDMA, wireless sensor networks