Bio-inspired apatite particles limit skin penetration of drugs for dermatology applications

Choimet, Maëla; Tourrette, Audrey; Marsan, Olivier; Rassu, Giovanna; Drouet, Christophe

doi:10.1016/j.actbio.2020.05.010

Cited by 9 publications

(10 citation statements)

References 40 publications

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“…http://www.minsocam.org/ diagnosis (Mondéjar et al, 2007. The luminescence signal of REE in apatite has recently been exploited, coupled to Raman and histology analysis, to follow the skin penetration of apatite-based colloidal nanoparticles for dermatological applications (Choimet et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Time-resolved Raman and luminescence spectroscopy of synthetic REE-doped hydroxylapatites and natural apatites

Fau

Beyssac

Gauthier

et al. 2022

American Mineralogist

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Using continuous and time-resolved spectroscopy, we investigate Raman and luminescence signals from synthetic hydroxylapatites doped with trivalent REE including Dy 3+ , Eu 3+ , Nd 3+ , and Sm 3+ , as well as REE in natural apatites, with laser excitations at 532 nm and 785 nm. We demonstrate that time-resolved spectroscopy is an extremely efficient method to tone down the luminescence from Raman spectra or, alternatively, to investigate the luminescence signal without the interference from the Raman contribution. Time-resolved luminescence spectroscopy is found to be a powerful technique for generating specific high-quality luminescence spectra for the REE emission activators in apatites by using appropriate combinations of delay and gate width for the time synchronization of the laser pulse and ICCD detector. This allows for the unambiguous detection and identification of the activators by avoiding the overlapping of various emission signals in the luminescence spectra. This is particularly useful in the case of natural samples, which often have several activators for luminescence. In the case of synthetic REE-doped apatites, a quenching process for luminescence due to activator concentration is seen for Eu 3+ and Sm 3+ , i.e. the higher the concentration, the shorter the luminescence decay time. The interpretation of luminescence decay time in natural apatites is promising but more complex because of energy transfers between the various luminescence activators present in the crystal lattice. Luminescence is a powerful technique for detecting the presence of REE in apatites down to ppm levels, though quantifying the concentration is still a challenge.

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Section: Introductionmentioning

confidence: 99%

Time-resolved Raman and luminescence spectroscopy of synthetic REE-doped hydroxylapatites and natural apatites

Fau

Beyssac

Gauthier

et al. 2022

American Mineralogist

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“…The presence of a contribution on the XRD pattern at 2𝜃 = 23°c an also be noted, which can be attributed to some degree of or-dering of the long PEG chains in the dried state, in a similar way as previously reported. [20] Moreover, dynamic light scattering (DLS) was carried out to evaluate the hydrodynamic diameter D h of these NPs 1 to 6 which could be considered homogenous (polydispersity index (PdI) between 0.2 and 0.3) with a D h value ranging from 391 nm for the ratio equal to 0.005 down to 126 nm for the highest ratio (Table 1). DLS is more suited to analyze spherical objects, thus transmission electron microscopy (TEM) analyses were performed to in depth examine the particles size and shape (Figure 2a).…”

Section: Resultsmentioning

confidence: 99%

“…We chose to use P(PEG) rather than AEP as apatite bonding moiety due to better stabilizing properties reported. [ 20 ] The addition of the peptide on the free amine of AEP would disrupt the electrostatic repulsion and thus the stabilization of the apatite NPs because ammonium ions would not be present on the apatite surface. The use of P(PEG) would not only allow the addition of the peptide without disrupting its stabilizing effect to obtai NPs of controlled size, but also would move the peptide away from the apatite surface thus limiting apatite/peptide interactions.…”

Section: Resultsmentioning

confidence: 99%

“…[18] Also, phosphonated polyethylene glycol (P(PEG)) can prevent particle agglomeration, by steric hindrance. [20] In this view, the length of the PEGylated chain allowed obtaining submicron to nanoscale colloidal particles relevant to dermatology, [20] hematology, [21] or oncology. [10,22,23] However, these stabilizing agents being not bioactive by themselves, it was necessary to co-adsorb other molecular moieties thus leading to competitive adsorption phenomena.…”

Section: Introductionmentioning

confidence: 99%

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One‐Pot Synthesis of Bioinspired Peptide‐Decorated Apatite Nanoparticles for Nanomedicine

Guérin,

Lebrun,

Kuhn

et al. 2023

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Hybrid organic–inorganic bio‐inspired apatite nanoparticles (NPs) are attractive for biomedical applications and especially in nanomedicine. Unfortunately, their applications in nanomedicine are limited by their broad particle size distributions and uncontrolled drug loading due to their multistep synthesis process. Besides, very few attempts at exposing bioactive peptides on apatite NPs are made. In this work, an original one‐pot synthesis of well‐defined bioactive hybrid NPs composed of a mineral core of bioinspired apatite surrounded by an organic corona of bioactive peptides is reported. Dual stabilizing‐bioactive agents, phosphonated polyethylene glycol‐peptide conjugates, are prepared and directly used during apatite precipitation i) to form the organic corona during apatite precipitation, driving the size and shape of resulting hybrid NPs with colloidal stabilization and ii) to expose peptide moieties (RGD or YIGSR sequences) at the NPs periphery in view of conferring additional surface properties to enhance their interaction with cells. Here, the success of this approach is demonstrated, the functionalized NPs are fully characterized by Fourier‐transform infrared, Raman, X‐ray diffraction, solid and liquid state NMR, transmission electron microscopy, and dynamic light scattering, and their interaction with fibroblast cells is followed, unveiling a synergistic proliferative effect.

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“…However, for the comparison of products, the equivalence parameters of drug uptake and clearance, could conceivably be compared, in situ. As an example, Essendoubi et al used MCR-ALS for the analysis of CRM penetration profiles of free and an encapsulated form of retinol in sections of frozen and living tissue, demonstrating a higher permeation efficiency of the encapsulated form [89], and a number of further studies have used CRM to monitor the penetration of nanoformulations [90,91].…”

Section: Raman Microspectroscopic Analysis Of Skin Penetration and Pe...mentioning

confidence: 99%

Monitoring Dermal Penetration and Permeation Kinetics of Topical Products; the Role of Raman Microspectroscopy

Byrne¹,

Bielfeldt²,

Bonnier³

et al. 2022

SSRN Journal

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Bio-inspired apatite particles limit skin penetration of drugs for dermatology applications

Cited by 9 publications

References 40 publications

Time-resolved Raman and luminescence spectroscopy of synthetic REE-doped hydroxylapatites and natural apatites

Time-resolved Raman and luminescence spectroscopy of synthetic REE-doped hydroxylapatites and natural apatites

One‐Pot Synthesis of Bioinspired Peptide‐Decorated Apatite Nanoparticles for Nanomedicine

Monitoring Dermal Penetration and Permeation Kinetics of Topical Products; the Role of Raman Microspectroscopy

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