Bio-Separated and Gate-Free 2D MoS2 Biosensor Array for Ultrasensitive Detection of BRCA1

Abstract

2D molybdenum disulfide (MoS2)-based thin film transistors are widely used in biosensing, and many efforts have been made to improve the detection limit and linear range. However, in addition to the complexity of device technology and biological modification, the compatibility of the physical device with biological solutions and device reusability have rarely been considered. Herein, we designed and synthesized an array of MoS2 by employing a simple-patterned chemical vapor deposition growth method and meanwhile exploited a one-step biomodification in a sensing pad based on DNA tetrahedron probes to form a bio-separated sensing part. This solves the signal interference, solution erosion, and instability of semiconductor-based biosensors after contacting biological solutions, and also allows physical devices to be reused. Furthermore, the gate-free detection structure that we first proposed for DNA (BRCA1) detection demonstrates ultrasensitive detection over a broad range of 1 fM to 1 μM with a good linear response of R2 = 0.98. Our findings provide a practical solution for high-performance, low-cost, biocompatible, reusable, and bio-separated biosensor platforms.

Review on two-dimensional material-based field-effect transistor biosensors: accomplishments, mechanisms, and perspectives

“…This method avoids non-specific adsorption of target molecules and ensures the purity of the response signal. The functionalization method of immobilizing probes based on Au–S bond or Pt–S bond [ 162 – 164 ]: metal nanoparticles (such as AuNPs (Fig. 6 f) [ 15 ], PtNPs [ 151 ]) are first deposited on the surface of the sensing material.…”

“…The functionalization method of immobilizing probes based on Au–S bond or Pt–S bond [ 162 – 164 ]: metal nanoparticles (such as AuNPs (Fig. 6 f) [ 15 ], PtNPs [ 151 ]) are first deposited on the surface of the sensing material.…”

2D semiconductors for specific electronic applications: from device to system

“…The principle is similar to the detection of some molecules or ions as chemical sensors 126 . One of the problems of detection in solution is that long-time immersion can easily damage the channel material or structure of the transistor, thus the controllability of the device is poor and non-reusable 127 . 2D semiconductors can also provide sensitive detection of pressure 128 , yet pressure puts high demands on the stability of the device as well.…”

Molybdenum disulfide (MoS2)-based nanostructures for tissue engineering applications: prospects and challenges

“…122 Recently, these properties are being explored for photothermal therapies 123,124 and applications concerning cellular interactions. 125,126 In terms of cellular interaction, cell adhesion and proliferation plays a crucial role in getting adequate cellular responses. Understanding the mechanistic pathway concerning cell proliferation upon interaction with MoS 2 could lead to different application-oriented strategies.…”