Bio-source substances against postharvest diseases of fruits: Mechanisms, applications and perspectives

Chen, Daoguo; Chen, Tong; Chen, Yong; Zhang, Zhanquan; Li, Boqiang; Tian, Shiping

doi:10.1016/j.postharvbio.2023.112240

Cited by 29 publications

(4 citation statements)

References 60 publications

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“…In addition, the polyene core is undergone to post-PKS modification by various enzymes including cytochrome P450, sulfonyl transferases, and glycosyl transferases 15 . Among the antifungal polyenes, macrocyclic polyenes such as amphotericin B, nystatin A1, natamycin and lucensomycin have been widely used to control fungi, such as being used to treat severe fungal infections, as a preservative in food, or to control fungal diseases in crops 14 , 16 , 17 . In addition, linear aminopolyol polyenes with amino or guanidino moieties such as linearmycin and mediomycin have potential to be applied as a wide range of antibiotics to have antifungal activity as well as antibacterial activity 18 , 19 .…”

Section: Introductionmentioning

confidence: 99%

“…Natamycin, a polyene macrolide compound biosynthesized by S. chattanoogensis L10, has been reported to exhibit inhibitory effects against a spectrum of phytopathogenic fungi. Notably, it impedes the growth of Penicillium digitatum , Rhizopus stolonifer , Alternaria alternata , and Botrytis cinerea , causative agents of fruit decay, black bread mold, leaf spots, and gray mold, respectively 17 . In a similar vein, Lucensomycin, another polyene macrolide compound synthesized by S. plumbeus strain CA5, has demonstrated its promise in inhibiting the growth of Botrytis cinerea , responsible for causing gray mold 21 .…”

Section: Introductionmentioning

confidence: 99%

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Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

Park,

Kang,

Choi

et al. 2024

Sci Rep

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Soil microorganisms with diverse bioactive compounds such as Streptomyces are appreciated as valuable resources for the discovery of eco-friendly fungicides. This study isolated a novel Streptomyces from soil samples collected in the organic green tea fields in South Korea. The isolation process involved antifungal activity screening around 2400 culture extracts, revealing a strain designated as S. collinus Inha504 with remarkable antifungal activity against diverse phytopathogenic fungi. S. collinus Inha504 not only inhibited seven phytopathogenic fungi including Fusarium oxysporum and Aspergillus niger in bioassays and but also showed a control effect against F. oxysporum infected red pepper, strawberry, and tomato in the in vivo pot test. Genome mining of S. collinus Inha504 revealed the presence of the biosynthetic gene cluster (BGC) in the chromosome encoding a polyene macrolide which is highly homologous to the lucensomycin (LCM), a compound known for effective in crop disease control. Through genetic confirmation and bioassays, the antifungal activity of S. collinus Inha504 was attributed to the presence of LCM BGC in the chromosome. These results could serve as an effective strategy to select novel Streptomyces strains with valuable biological activity through bioassay-based screening and identify biosynthetic gene clusters responsible for the metabolites using genome mining approach.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

Park,

Kang,

Choi

et al. 2024

Sci Rep

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“…Bio-sourced antifungal substances have attracted broad attention recently for their prominent properties, such as being non-toxic, readily available, and environmentally friendly (Chen et al 2023a ). To date, a series of bio-sourced substances have been reported with remarkable antifungal activities and promising potential for controlling B. cinerea (Wang et al 2020 ; Cui et al 2021 ; Sun et al 2021 ; Li et al 2022a ; Zhang et al 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system

Zhang,

Chen

et al. 2024

Mol Horticulture

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Botrytis cinerea is one of the most destructive phytopathogenic fungi, causing significant losses to horticultural crops. As a necrotrophic fungus, B. cinerea obtains nutrients by killing host cells. Secreted cell death-inducing proteins (CDIPs) play a crucial role in necrotrophic infection; however, only a limited number have been reported. For high-throughput CDIP screening, we optimized the prokaryotic expression system and compared its efficiency with other commonly used protein expression systems. The optimized prokaryotic expression system showed superior effectiveness and efficiency and was selected for subsequent CDIP screening. The screening system verified fifty-five candidate proteins and identified two novel SGNH family CDIPs: BcRAE and BcFAT. BcRAE and BcFAT exhibited high expression levels throughout the infection process. Site-directed mutagenesis targeting conserved Ser residues abolished the cell death-inducing activity of both BcRAE and BcFAT. Moreover, the transient expression of BcRAE and BcFAT in plants enhanced plant resistance against B. cinerea without inducing cell death, independent of their enzymatic activities. Our results suggest a high-efficiency screening system for high-throughput CDIP screening and provide new targets for further study of B. cinerea-plant interactions.

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“…In addition, the polyene core is undergone to post-PKS modi cation by various enzymes including cytochrome P450, sulfonyl transferases, and glycosyl transferases [15] . Among the antifungal polyenes, macrocyclic polyenes such as amphotericin B, nystatin A1, natamycin and lucensomycin have been widely used to control fungi, such as being used to treat severe fungal infections, as a preservative in food, or to control fungal diseases in crops [14],[16], [17] . In addition, linear aminopolyol polyenes with amino or guanidino moieties such as linearmycin and mediomycin have potential to be applied as a wide range of antibiotics to have antifungal activity as well as antifungal activity [18], [19] .…”

Section: Introductionmentioning

confidence: 99%

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

Park,

Kang,

Choi

et al. 2023

Preprint

View full text Add to dashboard Cite

Soil microorganisms with diverse bioactive compounds such as Streptomyces are appreciated as valuable resources for the discovery of eco-friendly fungicides. This study isolated a novel Streptomyces from soil samples collected in the organic green tea fields in South Korea. The isolation process involved antifungal activity screening around 2400 culture extracts, revealing a strain designated as S. collinus Inha504 with remarkable antifungal activity against diverse phytopathogenic fungi. S. collinus Inha504 inhibited seven phytopathogenic fungi, including Fusarium oxysporum and Aspergillus niger, in bioassays and showed a control effect against F. oxysporum infected red pepper, strawberry, and tomato in the in vivo pot test. Genome mining of S. collinus Inha504 revealed the presence of the biosynthetic gene cluster (BGC) in the chromosome encoding a polyene macrolide which is highly homologous to the lucensomycin (LCM), a compound known for effective in crop disease control. Through genetic confirmation and bioassays, the antifungal activity of S. collinus Inha504 was attributed to the presence of LCM BGC in the chromosome. These results could serve as an effective strategy to select novel Streptomyces strains with valuable biological activity through bioassay-based screening and identify biosynthetic gene clusters responsible for the metabolites using genome mining approach.

show abstract

Bio-source substances against postharvest diseases of fruits: Mechanisms, applications and perspectives

Cited by 29 publications

References 60 publications

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

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