Bioaccessibility and oral immunization efficacy of a chimeric protein vaccine against Ascaris suum

Castro, Joseane Camilla de; Magalhães, Luísa Mourão Dias; Almeida, Raquel M. de; Oliveira, Fabrício Marcus Silva; Nogueira, Denise Silva; Gazzinelli-Guimarães, Ana Clara; Kraemer, Lucas; Barbosa, Fernando Sérgio; Santos, Flavianne Vieira; Minighin, Elaine Carvalho; Bueno, Lilian Lacerda; Bartholomeu, Daniella Castanheira; Labanca, Renata Adriana; Fujiwara, Ricardo Toshio

doi:10.1016/j.micinf.2022.105042

Cited by 3 publications

(2 citation statements)

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“…2021), and finally as an important tool for preclinical vaccine trials (Castro et al, 2023;Gazzinelli-Guimaraes et al, 2018;Gazzinelli-Guimaraes, Nogueira, et al, 2021;Wei et al, 2017), because it allows the evaluation of infection-driven pathogenesis as well as vaccine-driven immunogenicity at a tissue level.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, the Ascaris mouse model (Fig. 1) has been widely utilized for studies of liver inflammation (Deslyper et al., 2019; Oliveira, Kramer, et al., 2022), type 2 immune responses in the lung tissue (Gazzinelli‐Guimaraes et al., 2013; Gazzinelli‐Guimaraes et al., 2019; Weatherhead et al., 2018), host genetics underlying susceptibility to infection (Dold et al., 2010; Lewis et al., 2006; Oliveira, Nogueira, et al., 2022), re‐infections (Nogueira et al., 2016), and co‐infections and interactions (Gazzinelli‐Guimaraes et al., 2017; Gazzinelli‐Guimaraes et al., 2019; Oliveira et al., 2019; Vieira‐Santos et al., 2021), and finally as an important tool for preclinical vaccine trials (Castro et al., 2023; Gazzinelli‐Guimaraes et al., 2018; Gazzinelli‐Guimaraes, Nogueira, et al., 2021; Wei et al., 2017), because it allows the evaluation of infection‐driven pathogenesis as well as vaccine‐driven immunogenicity at a tissue level.…”

Section: Introductionmentioning

confidence: 99%

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Ascaris Mouse Model Protocols: Advancing Research on Larval Ascariasis Biology

de Almeida Lopes,

Wang,

Liffner

et al. 2024

Current Protocols

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Ascariasis, caused by both Ascaris lumbricoides and Ascaris suum, is the most prevalent parasitic disease worldwide, affecting both human and porcine populations. However, due to the difficulties of assessing the early events of infection in humans, most studies of human ascariasis have been restricted to the chronic intestinal phase. Therefore, the Ascaris mouse model has become a fundamental tool for investigating the immunobiology and pathogenesis of the early infection stage referred to as larval ascariasis because of the model's practicality and ability to replicate the natural processes involved. The Ascaris mouse model has been widely used to explore factors such as infection resistance/susceptibility, liver inflammation, lung immune‐mediated pathology, and co‐infections and, notably, as a pivotal element in preclinical vaccine trials. Exploring the immunobiology of larval ascariasis may offer new insights into disease development and provide a substantial understanding of key components that trigger a protective immune response. This article focuses on creating a comprehensive guide for conducting Ascaris experimental infections in the laboratory as a foundation for future research efforts. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Acquisition and embryonation of Ascaris suum eggs from adult femalesAlternate Protocol: Cleaning and purification of Ascaris suum from female A. suum uteriBasic Protocol 2: Preparation of Ascaris suum eggs and murine infectionBasic Protocol 3: Measurement of larval burden and Ascaris‐larva‐induced pathogenesisBasic Protocol 4: In vitro hatching and purification of Ascaris L3 larvaeSupport Protocol: Preparation of crude antigen from Ascaris infectious stagesBasic Protocol 5: Ultrastructure‐expansion microscopy (U‐ExM) of Ascaris suum larval stages

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ascaris Mouse Model Protocols: Advancing Research on Larval Ascariasis Biology

de Almeida Lopes,

Wang,

Liffner

et al. 2024

Current Protocols

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Evaluation of reference genes for gene expression analysis by real-time quantitative PCR (qPCR) in different tissues from mice infected by Ascaris suum

Souza,

Lopes,

Leal-Silva

et al. 2024

Microbial Pathogenesis

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In silico design of a polypeptide as a vaccine candidate against ascariasis

Evangelista

Vliet

Lawton

et al. 2023

Sci Rep

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Ascariasis is the most prevalent zoonotic helminthic disease worldwide, and is responsible for nutritional deficiencies, particularly hindering the physical and neurological development of children. The appearance of anthelmintic resistance in Ascaris is a risk for the target of eliminating ascariasis as a public health problem by 2030 set by the World Health Organisation. The development of a vaccine could be key to achieving this target. Here we have applied an in silico approach to design a multi-epitope polypeptide that contains T-cell and B-cell epitopes of reported novel potential vaccination targets, alongside epitopes from established vaccination candidates. An artificial toll-like receptor-4 (TLR4) adjuvant (RS09) was added to improve immunogenicity. The constructed peptide was found to be non-allergic, non-toxic, with adequate antigenic and physicochemical characteristics, such as solubility and potential expression in Escherichia coli. A tertiary structure of the polypeptide was used to predict the presence of discontinuous B-cell epitopes and to confirm the molecular binding stability with TLR2 and TLR4 molecules. Immune simulations predicted an increase in B-cell and T-cell immune response after injection. This polypeptide can now be validated experimentally and compared to other vaccine candidates to assess its possible impact in human health.

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Bioaccessibility and oral immunization efficacy of a chimeric protein vaccine against Ascaris suum

Cited by 3 publications

References 31 publications

Ascaris Mouse Model Protocols: Advancing Research on Larval Ascariasis Biology

Ascaris Mouse Model Protocols: Advancing Research on Larval Ascariasis Biology

Evaluation of reference genes for gene expression analysis by real-time quantitative PCR (qPCR) in different tissues from mice infected by Ascaris suum

In silico design of a polypeptide as a vaccine candidate against ascariasis

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