Bioactive and Functional Oligomers Derived from Natural PHA and Their Synthetic Analogs

Ekere, Anabel Itohowo; Kurcok, Piotr; Adamus, Grażyna; Konieczny, Tomasz; Zięba, Magdalena; Chaber, Paweł; Tchuenbou-Magaia, Fideline; Kowalczuk, Marek

doi:10.1201/9781003087663-5

Cited by 5 publications

(14 citation statements)

References 89 publications

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“…Subsequently, after 24 h, a gradual decline in cell viability was observed for all growth conditions. Under the same growth conditions, quite a similar growth pattern to that seen in this study was previously reported by Ekere et al [23] when low-density PE fragments were used as the sole carbon source, although in their study better cell growth was observed with BSM. The results presented in this study (Figure 5) show a similar pattern for both controls and both media supplemented with PE-T.…”

Section: Microbial Growth Analysissupporting

confidence: 90%

“…There is the likelihood that these bonds suggest the PHBV nature of this PHA sample. Another report [23] also described similar observations for the production of P(HB-co-HV). The differences between the FTIR spectra of the starting PE-T material and the FTIR spectra of the resulting PHA sample are highly noticeable, and depict the purity of the PHA sample produced from PE-T.…”

Section: Pha Identification and Characterisationsupporting

confidence: 69%

“…PHA production from waste Tetra Pak ® could be an important economical alternative to the other recycling methods of polyethylene polymer materials. We have extensively described the use of oxidised polypropylene (PP) as a feedstock for PHA production in previous publications [22], as well as a recycling method for controlled oxidative fragmentation of LDPE plastics by Cupriavidus necator [23]. The PHB producer Cupriavidus necator consistently stands out for its ability to produce PHA from multiple carbon sources, including sugars and fatty acids [23].…”

Section: Introductionmentioning

confidence: 99%

“…We have extensively described the use of oxidised polypropylene (PP) as a feedstock for PHA production in previous publications [22], as well as a recycling method for controlled oxidative fragmentation of LDPE plastics by Cupriavidus necator [23]. The PHB producer Cupriavidus necator consistently stands out for its ability to produce PHA from multiple carbon sources, including sugars and fatty acids [23]. It is genetically stable, and is one of the most studied microbes used in this area of study, making it a potential candidate for industrial PHA production [22,23].…”

Section: Introductionmentioning

confidence: 99%

“…The PHB producer Cupriavidus necator consistently stands out for its ability to produce PHA from multiple carbon sources, including sugars and fatty acids [23]. It is genetically stable, and is one of the most studied microbes used in this area of study, making it a potential candidate for industrial PHA production [22,23]. This strain was used as a biocatalyst to upcycle LDPE fragments in Tetra Pak ® waste to value-added PHA.…”

Section: Introductionmentioning

confidence: 99%

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Bioconversion Process of Polyethylene from Waste Tetra Pak® Packaging to Polyhydroxyalkanoates

Ekere

Johnston²,

Tchuenbou-Magaia

et al. 2022

Polymers

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Presented herein are the results of a novel recycling method for waste Tetra Pak® packaging materials. The polyethylene (PE-T) component of this packaging material, obtained via a separation process using a “solvents method”, was used as a carbon source for the biosynthesis of polyhydroxyalkanoates (PHAs) by the bacterial strain Cupriavidus necator H16. Bacteria were grown for 48–72 h, at 30 °C, in TSB (nitrogen-rich) or BSM (nitrogen-limited) media supplemented with PE-T. Growth was monitored by viable counting. It was demonstrated that C. necator utilised PE-T in both growth media, but was only able to accumulate 40% w/w PHA in TSB supplemented with PE-T. Only 1.5% w/w PHA was accumulated in the TSB control, and no PHA was detected in the BSM control. Extracted biopolymers were characterised by nuclear magnetic resonance (NMR), Fourier-transform infrared (FTIR) spectroscopy, electrospray tandem mass spectrometry (ESI-MS/MS), gel permeation chromatography (GPC), and accelerator mass spectrometry (AMS). The characterisation of PHA by ESI-MS/MS revealed that PHA produced by C. necator in TSB supplemented with PE-T contained 3-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate co-monomeric units. AMS analysis also confirmed the presence of 96.73% modern carbon and 3.27% old carbon in PHA derived from Tetra Pak®. Thus, this study demonstrates the feasibility of our proposed recycling method for waste Tetra Pak® packaging materials, alongside its potential for producing value-added PHA, and the ability of 14C analysis in validating this bioconversion process.

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Section: Microbial Growth Analysissupporting

confidence: 90%

Section: Pha Identification and Characterisationsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Bioconversion Process of Polyethylene from Waste Tetra Pak® Packaging to Polyhydroxyalkanoates

Ekere

Johnston²,

Tchuenbou-Magaia

et al. 2022

Polymers

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Balancing Melt Solubility and Morphology in Epitaxial Nucleation: The Case of Nicotinic Acid and Poly(hydroxybutyrate-co-hydroxyhexanoate)

Bledsoe,

Crane,

Drewke

et al. 2023

ACS Appl. Polym. Mater.

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Nicotinic acid was evaluated as a melt-soluble nucleator for a poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) copolymer for melt-processing applications. A series of nicotinic acid concentrations (1–5 wt %) were analyzed to determine the best concentration for overall nucleation performance. 2 w/w% nicotinic acid was found to be the optimal concentration, successfully crystallizing PHBHHx at a peak temperature of 73 °C under nonisothermal conditions. When extrusion is performed at 150 °C, 2 w/w% of nicotinic acid occupies an optimal concentration within the polymer where all the nicotinic acid is dissolved in the PHBHHx matrix, which is not the case at higher concentrations. 2 w/w% also recrystallizes rapidly to produce many fine, needle-like crystals that are highly active toward PHBHHx nucleation, which is not observed with other concentrations. Powder X-ray diffraction (PXRD) analysis of the differing nicotinic acid crystals determined that the (110) and (120) faces are likely responsible for nucleation. Gel permeation chromatography (GPC) analysis revealed a modest degradation of molecular weight, likely due to the E1cb degradation mechanism common in PHAs.

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From Anionic Ring-Opening Polymerization of β-Butyrolactone to Biodegradable Poly(hydroxyalkanoate)s: Our Contributions in This Field

et al. 2021

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The feasibility of synthesis of functionalized poly(3-hydroxybutanoic acid) analogue and its copolymers via ring-opening polymerization of β-butyrolactone mediated by activated anionic initiators is presented. Using these new synthetic approaches, polyesters with a defined chemical structure of the end groups, as well as block, graft, and random copolymers, have been obtained and characterized by modern instrumental techniques, with special emphasis on ESI-MS. The relationship between the structure and properties of the prepared polymeric materials is also discussed.

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Bioactive and Functional Oligomers Derived from Natural PHA and Their Synthetic Analogs

Cited by 5 publications

References 89 publications

Bioconversion Process of Polyethylene from Waste Tetra Pak® Packaging to Polyhydroxyalkanoates

Bioconversion Process of Polyethylene from Waste Tetra Pak® Packaging to Polyhydroxyalkanoates

Balancing Melt Solubility and Morphology in Epitaxial Nucleation: The Case of Nicotinic Acid and Poly(hydroxybutyrate-co-hydroxyhexanoate)

From Anionic Ring-Opening Polymerization of β-Butyrolactone to Biodegradable Poly(hydroxyalkanoate)s: Our Contributions in This Field

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