Bioactive Compound from Extract Filtrat Streptomyces sp.Sp1. as Biocontrol of Vibriosis on Larvae of Macrobrachium rosenbergii shrimps

Abstract

Production of Macrobrachium rosenbergii shrimp was limited by vibriosis disease. Streptomyces can be used as an alternative control of vibriosis which is caused by Vibrio anguillarum. Aplication of Streptomyces sp.1 culture could give significant different impacts (p<0.05) on the percentage of survival (SR) of shrimp larvae that had been infected by V. anguillarum compared to the control. This study was conducted to identify and determine bioactive compound of Streptomyces sp.1. The presence of bioactive compound was confirmed by gas chromatography-mass spectrometry (GC-MS) analysis. Identification of Streptomyces sp.1 by 16S rRNA partial gene sequencing method. Streptomyces sp.1 was identified to be Streptomyces sp.Sp1. There were 35 chemical compounds identified, the chemical compounds which have antimicrobial properties identified in the n butanol extract of Streptomyces sp.Sp1 are 3-Hexanone, 2-methyl (43%), n Butyl ether (19%) Nonane (CAS) n-Nonane (5.6%), Decane (CAS) n-Decane (3.2%), Hexacosane (CAS) n-Hexacosane (1.9%), Tetracontane (1.3%), Heneicosane (1.9%), Hexadeconoic acid, methyl ester (0.3%), Butane,1,1-dibutoxy (0.3%), and Limonene (0.3%). Benzeneacetic acid, 3-methoxy-.alpha.,4-bis[(tri (0.3%). These results suggested that Streptomyces sp.Sp1 had good general antimicroba activity and might have potential biocontrol antagonist Vibrio anguillarum.

Analysis of Streptomyces Volatilomes Using Global Molecular Networking Reveals the Presence of Metabolites with Diverse Biological Activities

“…1,2,3-Trimethyl-cyclohexane has been described in certain flowering plants and fruit trees ( 111 , 113 , 114 ) and was also reported as one of the major components of Streptomyces extracts possessing anti-methicillin-resistant Staphylococcus aureus (MRSA) activities ( 115 ). Dodecanoic acid methyl ester is also widely found in plant oils ( 116 ), and its production in Streptomyces has been reported ( 117 ), but currently there is no information on its function or biological activity. Methyl tetradecanoate has been reported in multiple plant species ( 93 , 118 ), Streptomyces ( 119 ), and insects ( 120 – 122 ).…”

Antimicrobial compounds of one Streptomyces celluloflavus strain isolated from Can Gio mangrove soil, Vietnam

“…Next, Hassan et al used GC-MS to analyze the composition of active ingredients obtained from the culture fluid of Bacillus cereus S1 strain isolated from Egyptian seawater, showing that Heptasiloxane, hexadecamethyl and some other substances, have anticoagulant, anti-inflammatory and inhibitory activities against both Gram-negative and Grampositive bacteria [24]. In particular, Kawuri and Darmayasa obtained and analyzed bioactive compounds from a strain of the genus Streptomyces that inhibited pathogenic bacteria to giant freshwater prawn larvae, including the presence of Heptasiloxane, hexadecamethyl [25].…”

Antibacterial and phytochemical constituent of Etlingera rubroloba A.D. Poulsen extract, an endemic ginger from Wallacea Region, Indonesia

“…While antibacterial compounds in rhizome were 1HCyclopenta [1,3] [3aS (3a.alpha., 3b.beta., 4.beta.,7.alpha., 7aS) or β-Cubebene (Constant et al 2019);1H-Cycloprop[e]azulen-7-ol, decahydro-1,1,7-trimethyl-4-methylene-, [1ar-(1a.alpha., 4a.alpha.,7.beta.,7a.beta.,7b.alpha. ) or spatulenol) (Cazella et al 2019); 1-Hexadecanol (fatty acid) (Togashi et al 2007), 5-Octadecene, (E)- (Naragani et al 2016), 9-Hexadecenoic acid, methyl ester, (Z)- (Rahman et al 2014), α -Pinene (Rivas da Silva et al 2012Dhar et al 2014), Bicyclo[3.1.0]hexane, 4methylene-1-(1-methylethyl)-or sabibine (Utegenova et al 2018), Camphene (de Freitas et al 2020, Copaene (Martins et al 2015, Cyclohexanol, 1-methyl-4-(1-methylethenyl)-, cis-or β-Terpinene (Guimarães et al 2019), Cyclopentaneacetic acid, 3-oxo-2-pentyl-, methyl ester (Shannon and Abu-Ghannam 2016), Decane (Kawuri and Darmayasa 2019), D-Limonene (Costa et al 2019;Han et al 2020), E-15-Heptadecenal (Supardy et al 2012), Ethylbenzene (Bellahcen et al 2019), Eucalyptol (Ghomi and Ahd 2010), γ -Terpinene (Guimarães et al 2019), Heptadecane, 8-methyl-(Ashraf et al 2018, Heptadecanoic acid, methyl ester (Köse et al 2016), Hexadecanoic acid, ethyl ester (Ghareeb et al 2022), Hexadecanoic acid, methyl ester (Ghareeb et al 2022), Isopropyl myristate (Cardoso et al 2006), Nonane (Kawuri and Darmayasa 2019), o-Xylene (Zayed and Samling 2016), Terpinen-4-ol (Cordeiro et al 2020, Tetradecane (Naragani et al 2016, Tridecanoic acid, 12-methyl-...…”