Bioactive Compounds and Antiradical Activity of the Rosa canina L. Leaf and Twig Extracts

Abstract

It is important to search for new sources of bioactive, natural compounds, because customers are paying more attention to food quality. Fruits and berries from horticultural plants are known to be good sources of agents beneficial for human well-being and could serve as natural preservatives in the food industry. However, more recent research indicates that other plant organs can also be rich in nutrients. Our study focused on characterizing an unexplored source, namely leaf and twig extracts from Rosa canina. The chemical composition of these extracts was analyzed and their in vitro activity measured. HPLC analysis of the content of phenolics, vitamins and amino acids revealed that the leaf and twig extracts were found to be rich in bioactive compounds with potent antioxidant properties. The greatest differences between bioactive phenolic compounds in leaf and twig extracts related mainly to p-coumaric acid, myricetin, ellagic acid, cyanidin, procyanidin and quercetin, whereas salicylic acid levels were similar in both types of extract. Interactions with human serum albumin were investigated, and some conformational changes in protein structure were observed. Further analysis (lipid peroxidation, protein carbonylation, thiol group oxidation, DPPH inhibition and ROS inhibition) confirmed that both leaf and twig extracts exhibited antioxidant and antiradical scavenging activities. Cytotoxicity and hemotoxicity assays confirmed very low toxicity of the extracts towards human cells over the range of concentrations tested. Our results indicate that both extracts could serve as non-toxic sources of bioactive compounds with antiradical properties.

Rosehip (Rosa canina L.) Meal as a Natural Antioxidant on Lipid and Protein Quality and Shelf-Life of Polyunsaturated Fatty Acids Enriched Eggs

“…In some cultures, rosehip has been used as a medicinal remedy for over 2000 years, but the actual consumption of this fruit is very popular in European countries like Romania, Poland, Portugal, Germany, Finland, and Sweden [ 8 ]. Rosehip is very rich in vitamins (A, B, C, D, and E), minerals (Ca, Fe, K, Mg, Mn, S, Si, and Se), polyphenols, and has high antioxidant capacity [ 9 , 10 , 11 ], with a moderate level of amino acids and fatty acids. Currently, rosehip fruits are used in the fruit processing industry for different purposes [ 8 ].…”

Valorization of Rose Hip (Rosa canina) Puree Co-Product in Enriched Corn Extrudates

“…Rose hips, the berry-fruits of many rose bushes species which belongs to Rosaceae family, and to the Rosa genus with over 150 species, grows in the northern hemisphere, including Europe, the Middle East, America, and Asia [13]. A large body of literature highlighted that rose hips are high in vitamins C, B, D, A, and E, as well as minerals (Mg, Ca, Mn, S, Si, K, Fe, and Se), phenolic compounds, and carotenoids [18][19][20][21][22][23]. They also contain organic acids, pectin, sugars, amino acids, and essential oils [24,25].…”

“…With respect to minerals content, in our recent study Igual et al [30], we emphasized that rose hip (Rosa canina) is a rich source in macroelements such as: Ca (7367.09 µ g/g), K (6785.77 µ g/g), Mg (1558.9 µ g/g), and microelements Fe (98.36 µ g/g) and Mn (127.56 µ g/g). Macro elements have a positive influence on A large body of literature highlighted that rose hips are high in vitamins C, B, D, A, and E, as well as minerals (Mg, Ca, Mn, S, Si, K, Fe, and Se), phenolic compounds, and carotenoids [18][19][20][21][22][23]. They also contain organic acids, pectin, sugars, amino acids, and essential oils [24,25].…”

Hippophae rhamnoides L. leaf and twig extracts as rich sources of nutrients and bioactive compounds with antioxidant activity

“…HPLC analysis were performed as previously described 57 . The HPLC system (Summit × 2 Dual-Gradient System, Dionex, Sunnyvale, CA, USA) was equipped with a photodiode-array detector (PDA100 DAD) and fluorescence detector (RF-2000).…”