Bioactive compounds and molecular diversity of endophytic actinobacteria isolated from desert plants

Natsagdorj, Oyunbileg; Davaapurev, Bekh-Ochir; Tsetseg, Baljinova; Iizaka, Yohei; Fukumoto, Atsushi; Kato, Fumio; Anzai, Yojiro; Batkhuu, Javzan

doi:10.1088/1755-1315/908/1/012008

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…These genera also play important roles in plant–microbe interactions. Actinocatenispora as an actinobacterium exhibits diverse metabolic capabilities and can contribute to plant growth promotion and disease suppression (Natsagdorj et al., 2021). Neorhizobium , on the other hand, is a nitrogen‐fixing bacterium that forms symbiotic associations with leguminous plants, providing them with a vital source of nitrogen (Mousavi et al., 2014).…”

Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity of latent bacteria in coconut leaves associated with lethal yellowing disease symptoms in Kenya

Omar,

Neondo,

Mweu

et al. 2024

Plant Pathology

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Coconut (Cocos nucifera) is an economically important palm tree with diverse applications. However, limited scientific research exists on coconut diseases in the Kenyan coastal region. This cross‐sectional study aimed to investigate the genetic diversity of latent bacteria associated with yellowing symptoms in coconut plants along the Kenyan coast. Sixty‐two samples with symptoms were collected and their bacterial diversity assessed using culture‐independent methods. DNA was extracted from controls and symptomatic samples using the cetyl trimethyl ammonium bromide (CTAB) method. Next‐generation sequencing with Illumina MiSeq was used to profile the bacterial communities using amplicons of 16S ribosomal RNA sequences (V4 region). Sequence data were analysed using the Quantitative Insights Into Microbial Ecology 2 (QIIME 2) pipeline. A total of 113,330 reads were obtained, which clustered into 285 Operational Taxonomic Units. Bacterial diversity was highest in Kilifi, followed by Kwale and Lamu, while control samples exhibited low bacterial diversity. Actinobacteria was the predominant phylum across all counties, and Streptomyces was the most abundant genus. Kilifi and Kwale counties were more affected than Lamu. This is a pioneer study that provides insight into the bacterial diversity associated with yellowing disease symptoms in coconut plants in Kenya and will help with future elucidation of the agents causing or exacerbating coconut disease symptoms.

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Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity of latent bacteria in coconut leaves associated with lethal yellowing disease symptoms in Kenya

Omar,

Neondo,

Mweu

et al. 2024

Plant Pathology

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“…Xie et al investigated the metabolites, transcriptome, and microbiome of 1-35-year-old poplar roots and found that the abundance of actinobacteria was strongly correlated with flavonoid biosynthesis with the increase in tree age (Xie et al, 2023). The study conducted by Natsagdorj et al utilizing HPLC analysis revealed that endophytic actinobacteria, which were isolated from desert plants, demonstrated the ability to synthesize flavonoids and phenolic compounds (Natsagdorj et al, 2021). The findings of these studies suggest a strong correlation between actinobacteria and the biosynthesis of flavonoids in the stems of Dendrobium.…”

Section: Discussionmentioning

confidence: 99%

Multi-omics profiling reveal responses of three major Dendrobium species from different growth years to medicinal components

Yuan,

Zuo,

Wan

et al. 2024

Front. Plant Sci.

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Dendrobium is a perennial herb found in Asia that is known for its medicinal and ornamental properties. Studies have shown that the stem is the primary medicinal component of Dendrobium spp. To investigate the effect of the species and age of Dendrobium (in years) on the content of its medicinal components, we collected the stems of 1-to-4-year-old D. officinale, D. moniliforme, and D. huoshanense, sequenced the transcriptome, metabolome, and microbiome, and analyzed the data in a comprehensive multi-omics study. We identified 10,426 differentially expressed genes (DEGs) with 644 differentially accumulated metabolites (DAMs) from 12 comparative groups and mapped the flavonoid pathway based on DEGs and DAMs. Transcriptomic and metabolomic data indicated a general trend of the accumulation of flavonoids exhibiting pharmacological effects in the three Dendrobium species. In addition, joint metabolome and microbiome analyses showed that actinobacteria was closely associated with flavonoid synthesis with increasing age. Our findings provide novel insights into the interactions of flavonoids of Dendrobium with the transcriptome and microbiome.

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“…Therefore, exploring natural bioactive compounds as potential new antibiotics is crucial to address this problem. Actinomycetes, which are frequently discovered in the tissues of plants (endophytes) and around plant roots (rhizosphere), have been explored as a promising source for the production of bioactive compounds (Quach et al, 2021;Natsagdorj et al, 2021;Elshafie et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of actinomycetes associated with clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli

Aji,

Azizah

2023

J. Nat. Scien. & Math. Res.

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Actinomycetes form associations with plants through colonizing plant tissues (endophytes) or by residing in the soil around some plants' roots (rhizosphere). Actinomycetes are known to produce antibacterial compounds. This study aimed to investigate the antibacterial activity of actinomycetes associated with the clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli. Actinomycetes were isolated from clove plants and the rhizosphere, and their antimicrobial activity against Staphylococcus aureus and Escherichia coli was evaluated using the agar plug method, where the presence of transparent zones around 10-day-old actinomycete growth indicated inhibition of bacterial growth. Four isolates showed inhibitory effects against Staphylococcus aureus, while only three isolates, B.4, T.3, and T.4, demonstrated inhibitory activity against Escherichia coli, as indicated by the presence of inhibition zones. Isolate T.3 exhibited the highest inhibition zone of 8.5 mm against S. aureus, whereas B.4 displayed the highest inhibition zone of 7.7 mm against E. coli. In conclusion, the actinomycetes found in clove plants (Syzygium aromaticum) demonstrate antibacterial properties against Staphylococcus aureus and Escherichia coli, indicating their potential as antibacterial agents.

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Bioactive compounds and molecular diversity of endophytic actinobacteria isolated from desert plants

Cited by 4 publications

References 11 publications

Assessment of genetic diversity of latent bacteria in coconut leaves associated with lethal yellowing disease symptoms in Kenya

Assessment of genetic diversity of latent bacteria in coconut leaves associated with lethal yellowing disease symptoms in Kenya

Multi-omics profiling reveal responses of three major Dendrobium species from different growth years to medicinal components

Antibacterial activity of actinomycetes associated with clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli

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