BIOACTIVE COMPOUNDS FROM TORBANGUN [Plectranthus amboinicus (Lour.) SPRENG] CHLOROFORM FRACTION INDUCE APOPTOSIS IN BREAST CANCER (MCF-7 CELLS) IN VITRO

Yulianto, Wahid; Andarwulan, Nuri; Giriwono, Puspo Edi; Pamungkas, Joko

doi:10.22146/tradmedj.24312

Cited by 10 publications

(9 citation statements)

References 30 publications

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“…P. amboinicus is used for the treatment of colds, coughs, asthma, and diseases of the respiratory tract in general, as well as to treat headaches, fever, skin diseases, and gastrointestinal disorders [ 17 , 18 , 19 ]. Thus, because it is a well-known species and used by the population, many studies on its effects already exist and demonstrate its antimicrobial and antiviral activities in vitro [ 20 , 21 ], activity against respiratory and gastrointestinal disorders, anticonvulsant, antitumor [ 22 , 23 ], analgesic, anti-inflammatory, and antioxidant activities [ 24 , 25 ]. These two species are also used in culinary as flavorings and in the preparation of some foods [ 17 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Seasonal Variations of Polyphenols Content, Sun Protection Factor and Antioxidant Activity of Two Lamiaceae Species

et al. 2021

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Secondary metabolite production by plants is influenced by external environmental factors that can change depending on the seasons, which makes it important to know how the plant, through its metabolism, is able to adapt to these variations. Mentha x villosa and Plectranthus amboinicus present in their chemical composition polyphenols, and through previous studies, it has been seen that these two species present promising in vitro photoprotective activity. The aim of this study was to evaluate seasonal alterations in photoprotective and antioxidant activities and the influence of factors such as precipitation levels and sun radiation incidence. Thus, polyphenol quantification, cromatographics (HPLC-DAD) and multivariate (PCA) analyses of extracts of the two species through twelve months were done. It was observed that the best months for photoprotective and antioxidant activities were September for M. villosa and July for P. amboinicus (SPF = 14.79). It was possible to conclude that solar radiation more clearly influences the production of phenolics and the increase of SPF in M. villosa, in addition to favoring the antioxidant activity of the two species, while precipitation seems to have no influence.

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Section: Introductionmentioning

confidence: 99%

Seasonal Variations of Polyphenols Content, Sun Protection Factor and Antioxidant Activity of Two Lamiaceae Species

et al. 2021

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“…The PCR reaction mix for identified HPV comprised 10 pmoL primers forward and reverses, Gotaq master mix PCR (Promega, USA), nuclease-free water, and DNA as a template. The primer used in this study is shown in Table-1 [ 9 - 11 ]. A total of 5 uL of DNA was amplified in a thermal cycler (VerityApplied Biosystem, USA) with PCR condition 95°C for 4 min, denaturation 95°C for 30 s, annealing 54°C for 30 s, and extension 72°C for 30 s. Stage denaturation until the extension was repeated to 40 cycles with amplification using electrophoresis gel agarose 1.8% later visualized in GelDoc2000.…”

Section: Methodsmentioning

confidence: 99%

Spontaneous expression of the gene of KI67 and P53 in cynomolgus monkeys infected with papillomavirus

et al. 2022

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Background and Aim: Cynomolgus monkeys (Macaca fascicularis) develop spontaneous infection of Papillomavirus (PV); thus, potentially beneficial for modeling human PV (HPV) infection study. Contrary to human origin, infection in cynomolgus monkeys does not always show evident clinical symptoms of cervical cancer. The absence of cervical cancer clinical symptoms leads us to investigate the molecular mechanism of the HPV infection in cynomolgus monkeys. This study aimed to investigate the messenger ribonucleic acid (mRNA) expression levels of KI67 and P53 genes, majorly known as biomarker oncogenesis of PV infection. Materials and Methods: The polymerase chain reaction (PCR) technique was used with MY11/MY09 primer to screen PV in cynomolgus monkey, further grouped as positive-PV and negative-PV infection groups. Real-time quantitative PCR was also applied to quantify the mRNA expression levels of KI67 and P53 genes in animals. Results: Increased expression of mRNA level of KI67 genes was significantly higher in Positive- PV group than negative-PV group. In contrast, the P53 mRNA expression level increased markedly higher in the negative-PV group than in the positive-PV group. Conclusion: Our study describes the potential of cynomolgus monkeys as a spontaneous oncogenesis model of PV infection-type. However, we used a limited number of cancer genetic markers. So, further study of other genetic markers is required to prove that cervical cancer could be developed naturally in cynomolgus monkeys.

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“…The spots of TLC from isolated results were identified by using UHPLC-MS/MS tandem mass spectrometer (UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS ThermoScientific, column Accucore phenyl hexyl, 100 x 2.1 mm, 2.6 µm). The principle of UHPLC-MS/MS refers to Yulianto et al (2017) and Maser et al (2015). Spots extract were diluted with 100 μL methanol and then filtrated by filter membrane 0.45 µm.…”

Section: Sample Identification With Bioactive Chemical Profile With Tmentioning

confidence: 99%

Identification of antioxidant bioactive compounds as potential functional food ingredient from kebar grass (Biophytum petersianum) by metabolomic approach

et al. 2022

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Kebar grass (Biophytum petersianum) is traditionally believed as a food ingredient to increase the health of the reproductive. The specific bioactive compounds with antioxidant effect in this plant have not been reported before. The objective of this research was to identify the specific bioactive compounds as a source of functional food ingredients of Kebar grass (KG) which have antioxidant activity by using a metabolomic approach. Samples chemical profile (extract and fraction) used HPLC, followed by metabolomic analysis by OPLS. Chosen samples were isolated by TLC and then identified by UHPLC-MS/MS. Specific bioactive compounds were identified from chromatogram with putative identification by MZmine referring to online databases. The metabolomic result obtained ethyl acetate fraction (FEA) as the sample that most contributed to antioxidant activity. FEA identification result showed ten specific bioactive compounds that are thought to contribute to antioxidant agents. Two of the compounds were identified as caffeic acid and cassiaoccidentalin A, while the other eight compounds have not been identified. The finding of cassiaoccidentalin A in KG is a novel finding and can be used as a specific marker for petersianum species of Biophytum genus and as a source of functional food ingredients as antioxidant agents to improve reproductive health.

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BIOACTIVE COMPOUNDS FROM TORBANGUN [Plectranthus amboinicus (Lour.) SPRENG] CHLOROFORM FRACTION INDUCE APOPTOSIS IN BREAST CANCER (MCF-7 CELLS) IN VITRO

Cited by 10 publications

References 30 publications

Seasonal Variations of Polyphenols Content, Sun Protection Factor and Antioxidant Activity of Two Lamiaceae Species

Seasonal Variations of Polyphenols Content, Sun Protection Factor and Antioxidant Activity of Two Lamiaceae Species

Spontaneous expression of the gene of KI67 and P53 in cynomolgus monkeys infected with papillomavirus

Identification of antioxidant bioactive compounds as potential functional food ingredient from kebar grass (Biophytum petersianum) by metabolomic approach

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