Bioactive compounds in hyperhydric and normal micropropagated shoots of Aronia melanocarpa (Michx.) Elliott

Sivanesan, Iyyakkannu; Saini, Ramesh Kumar; Kim, Doo Hwan

doi:10.1016/j.indcrop.2015.12.042

Cited by 20 publications

(14 citation statements)

References 29 publications

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“…In the present study, TDZ was the most benificial for shoot proliferation compared to KN and BA. The positive impact of TDZ on multiple shoot production has also been reported in several plants like Aronia melanocarpa [23], Bacopa monnieri [24], and Jeffersonia dubia [25]. In addition, several reports have confirmed that an auxin and cytokinin combination improves multiple shoot induction in members of the Boraginaceae [15,17,19,22].…”

Section: Discussionmentioning

confidence: 68%

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Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

Park

Kim

Saini

et al. 2019

IJMS

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The goal of this study was to establish an efficient protocol for the large-scale propagation of Mertensia maritima (L.) Gray, and evaluate the carotenoid, fatty acid, and tocopherol contents in the leaves of in vitro regenerated shoots. Surface-disinfected node and shoot tip explants were placed on semisolid Murashige and Skoog (MS) medium with 0–16 µM N6-benzyladenine (BA), kinetin, (KN), and thidiazuron (TDZ) alone, or in combination with, 1 or 2 µM α-naphthaleneacetic acid (NAA). Of the three different cytokinins employed, TDZ elicited the best results for axillary shoot proliferation. A maximum frequency of shoot initiation above 84%, with a mean of 8.9 and 4.8 shoots per node and shoot tip, respectively, was achieved on the culture medium supplemented with 4 µM TDZ. A combination of TDZ + NAA significantly increased the percentage of multiple shoot formation and number of shoots per explant. The best shoot induction response occurred on MS medium with 4 µM TDZ and 1 µM NAA. On this medium, the node (93.8%) and shoot tip (95.9%) explants produced an average of 17.7 and 8.6 shoots, respectively. The highest root induction frequency (97.4%) and number of roots per shoot (25.4), as well as the greatest root length (4.2 cm), were obtained on half-strength MS medium supplemented with 4 µM indole-3-butyric acid (IBA). The presence of six carotenoids and α-tocopherol in the leaf tissues of M. maritima was confirmed by HPLC. Gas chromatography-mass spectrometry analysis confirmed the presence of 10 fatty acids, including γ-linolenic acid and stearidonic acid in the leaf tissues of M. maritima. All-E-lutein (18.49 μg g−1 fresh weight, FW), α-tocopherol (3.82 μg g−1 FW) and α-linolenic acid (30.37%) were found to be the significant compounds in M. maritima. For the first time, a successful protocol has been established for the mass propagation of M. maritima with promising prospects for harnessing its bioactive reserves.

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Section: Discussionmentioning

confidence: 68%

“…Explants with vegetative meristems such as nodes and shoot tips are commonly used to obtain multiple shoots [23]. Shoot formation was not observed when node and shoot tip explants of the oyster plant were cultured on hormone-free growth medium, a similar result has been observed for other members of the Boraginaceae [18,22].…”

Section: Discussionmentioning

confidence: 77%

Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

Park

Kim

Saini

et al. 2019

IJMS

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“…A similar result has been previously reported in case of Ajuga multiflora also (Sivanesan et al, 2016a ). However, yet another report showed that the content of total carotenoids was significantly greater in leaves obtained from greenhouse-grown plants than leaves collected from in vitro -raised shoots of Aronia melanocarpa (Sivanesan et al, 2016b ). The content of α-tocopherol in the regenerated shoots (114.6 μg g −1 FW) of S. dasyphyllum was significantly higher (17.6 μg g −1 FW) compared to that obtained from greenhouse-grown plants.…”

Section: Discussionmentioning

confidence: 99%

“…The establishment of in vitro cell and organ cultures of S. dasyphyllum will be useful for secondary metabolite production. Several bioactive compounds like alkaloids, carotenoids, fatty acids, flavonoids, phenolic acids and tocopherols were obtained from in vitro shoot cultures of important plants (Jeong and Sivanesan, 2015 , 2016 ; Sivanesan et al, 2016a , b ; Dowom et al, 2017 ; Grzegorczyk-Karolak et al, 2017 ). Till date, no published reports are available on the micropropagation of S. dasyphyllum .…”

Section: Introductionmentioning

confidence: 99%

Micropropagation and Subsequent Enrichment of Carotenoids, Fatty Acids, and Tocopherol Contents in Sedum dasyphyllum L

et al. 2017

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A promising micropropagation protocol has been systematically established and demonstrated for the enhanced production of carotenoids, tocopherol and fatty acids in shoot tissues of Sedum dasyphyllum. Shoot tip explants were grown on Murashige and Skoog (MS) medium. Different concentrations of N6-benzyladenine (BA) or thidiazuron (TDZ) alone or in combination with α-naphthaleneacetic acid (NAA) were tested in order to stimulate multiple shoot production. Ideal shoot induction (100%) and maximized shoot numbers (36.4) were obtained on explants cultured on media incorporated with 2 μM BA and 1 μM NAA combinations. The in vitro-developed shoots rooted best on half-strength MS media incorporated with 2 μM indole 3-butyric acid. Plantlets were effectively acclimatized in the greenhouse with 100% survival rate. The composition and contents of bioactive compounds such as carotenoids, tocopherol and fatty acids in shoot tissues of S. dasyphyllum were investigated using HPLC and GC-MS. The most abundant carotenoid in the shoot tissue was all-E-lutein (40.3–70.5 μg g−1 FW) followed by 9′-Z-neoxanthin (5.3–9.9 μg g−1 FW), all-E-violaxanthin (4.4–8.2 μg g−1 FW), and all-E-β-carotene (1.6–3.6 μg g−1 FW). The α-tocopherol contents of in vitro-raised shoots was 6.5-fold higher than shoots of greenhouse-grown plants. The primary fatty acids found in shoot tissues were α-linolenic acid (32.0–39.3%), linoleic acid (27.4–38.2%), palmitic acid (13.3–15.5%), and stearic acid (5.2–12.2%). In all, summarizing the findings, the micropropagated S. dasyphyllum showed significant enrichment of valuable bioactive carotenoids (92.3 μg g−1 FW), tocopherols (14.6 μg g−1 FW), and α-linolenic acid (39.3%) compared to their greenhouse counterparts. The protocol demonstrated here could be applied for the mass propagation and production of enhanced bioactive compounds from S. dasyphyllum with credibility.

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“…The content of secondary compounds in cell and organ cultures was signicantly enhanced by optimizing the composition of the culture medium, incorporation of precursors and elicitors and providing appropriate culture conditions. 83,[88][89][90] NPs added to the plant in vitro culture medium may act as a nutrient source and an elicitor. Poborilova et al 67 reported that the addition of Al 2 O 3 NPs (10-100 mg mL À1 ) to tobacco cell suspension cultures signicantly increased the phenolic content.…”

Section: Nanomaterials Enhancement Of Secondary Metabolitesmentioning

confidence: 99%

Nanomaterials in plant tissue culture: the disclosed and undisclosed

2017

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Bioactive compounds in hyperhydric and normal micropropagated shoots of Aronia melanocarpa (Michx.) Elliott

Cited by 20 publications

References 29 publications

Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

Micropropagation and Subsequent Enrichment of Carotenoids, Fatty Acids, and Tocopherol Contents in Sedum dasyphyllum L

Nanomaterials in plant tissue culture: the disclosed and undisclosed

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