Bioactive dammarane-type triterpenoids derived from the acid hydrolysate of Gynostemma pentaphyllum saponins

Bai, Ming‐Sheng; Gao, Jin‐Min; Fan, Chunxiao; Shen, Yang; Gao, Zhang; Zheng, Cheng-Dong

doi:10.1016/j.foodchem.2009.06.033

Cited by 34 publications

(19 citation statements)

References 22 publications

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“…Furthermore, G. pentaphyllum was brought into the list of functional foods by Ministry of Public Health of China on March 5, 2002, and several brands of herbal products based on G. pentaphyllum such as Jiaogulan tea and total Jiaogulan saponin tablets have been available in East Asian markets since the early 1990s. In traditional Chinese medicine, recent reports have shown that G. pentaphyllum exhibits a variety of biological effects, such as immunopotentiating, cholesterol-lowering, antitumor, antioxidant, anti-inflammatory, anti-hyperlipidemic, anti-hypoglycemic and cardiovascular effects [17] . Gypenosides (GPs), the saponin extract derived from the herb G. pentaphyllum Makino, are believed to be one of the most pharmacologically active components in G. pentaphyllum.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of action of gypenosides on type 2 diabetes and non-alcoholic fatty liver disease in rats

et al. 2015

WJG

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AIM:To explore the mechanism of action of gypenosides (GPs) on type 2 diabetes mellitus and non-alcoholic fatty liver disease (T2DM-NAFLD) in rats. METHODS:Sixty rats were randomly divided into a healthy group, an untreated disease model group and GP-treatment groups. The study involved the evaluation of biochemical parameters, including serum aspartate transaminase (AST), alanine transferase (ALT), blood glucose (BG), triglycerides (TG) and total cholesterol (TC). Additionally, the protective effect of the treatments were confirmed histopathologically and the expression of TNF-α and NF-κB in the rat liver was analyzed using immunohistochemistry. The expression of proliferatoractivated receptor gamma (PPARγ) and cytochrome P450 (CYP450) 1A1 mRNA was determined by quantitative RT-PCR. RESULTS:GP treatments at oral doses of 200, 400, and 800 mg/kg per day significantly decreased the levels of serum AST and ALT (P < 0.05, P < 0.01), especially at the dose of 800 mg/kg per day. To a similar extent, GP at 800 mg/kg per day reduced the levels of BG (4.19 ± 0.47, P < 0.01), TG (80.08 ± 10.05, P < 0.01), TC (134.38 ± 16.39, P < 0.01) and serum insulin (42.01 ± 5.04, P < 0.01). The expression of TNF-α and NF-κB measured by immunohistochemistry was significantly reduced by GPs in a dose-dependent manner, and the expression of PPARγ and CYP4501A1 mRNA, as measured using quantitative real-time PCR, were significantly down-regulated by GPs. Moreover, GPs decreased the infiltration of liver fats and reversed the histopathological changes in a dosedependent manner. CONCLUSION:This study suggests that GPs have a protective effect against T2DM-NAFLD by down-regulating the expression of TNF-α and NF-κB proteins, and PPARγ and CYP4501A1 mRNAs. Core tip: Gypenosides (GPs) are one of the most pharmacologically active components in G. pentaphyllum . Basic Study ORIGINAL ARTICLEHe Q et al . Mechanism of action of GPs on T2DM-NAFLD Intervention with GPs significantly decreased the levels of aspartate aminotransferase, alanine aminotransferase, blood glucose, insulin, triglycerides and total cholesterol in type 2 diabetes mellitus and non-alcoholic fatty liver disease (T2DM-NAFLD) model rats, down-regulated the expression of TNF-α, NF-κB, rabbit anti-proliferator activated receptory (PPARγ) and rabbit anti-cytochrome P4501A1 (CYP1A1) mRNA, decreased the infiltration of liver fats and reversed the histopathological changes in a dose-dependent manner. These findings suggest that GPs have a protective effect against T2DM-NAFLD by downregulating the expression of TNF-α and NF-κB proteins, and PPARγ and CYP4501A1 mRNAs.He Q, Li JK, Li F, Li RG, Zhan GQ, Li G, Du WX, Tan HB. Mechanism of action of gypenosides on type 2 diabetes and non-alcoholic fatty liver disease in rats.

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Section: Introductionmentioning

confidence: 99%

Mechanism of action of gypenosides on type 2 diabetes and non-alcoholic fatty liver disease in rats

et al. 2015

WJG

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“…Bai investigated the crude saponins of Gynostemma pentaphyllum, three triterpenes of saponins exhibited inhibitory activity toward porcine pancreatic lipase [12]. The inhibitory activity of saponins from leaves of Acanthopanax sessiliflorus on PL has been reported, the result showed that sessiloside and chiisanoside inhibited pancreatic lipase activity in vitro, and addition of the saponin-rich Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In the previous reports, saponin prevented obesity induced by feeding a high-fat diet through inhibiting pancreatic lipase activity [8][9][10][11]. It has been reported that gypenosides exhibited inhibitory activity toward porcine pancreatic lipase, and the anti-hyperlipidemic effects of gypenosides in hyperlipidemic rats induced by high lipid diet have been verified [12,13]. The interactions between proanthocyanidins and PL and Epigallocatechin gallate (EGCG) have been investigated, including variant aspects of lipase conformation, activity, kinetics and thermodynamics [14,15], but there was rare study about the interactions between saponin and PL.…”

Section: Introductionmentioning

confidence: 90%

Hypolipidemic mechanism of gypenosides via inhibition of pancreatic lipase and reduction in cholesterol micellar solubility

Wang

et al. 2015

Eur Food Res Technol

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“…Besides the large numbers of methyl and methylene protons of the triterpene (Fig. 1B, region A), the HSQC spectrum showed the presence of olefinic signals (region D) indicating dammarane-type saponins (Bai et al, 2010;Ky et al, 2010;Norberg et al, 2004). In the HSQC-TOCSY spectrum (Fig.…”

Section: The Gp Extract Is Represented By Saponinsmentioning

confidence: 94%

“…A large number of saponins from GP, referred to as gypenosides, have been identified and characterized (Bai et al, 2010;Hu et al, 2010;Ky et al, 2010). These gypenosides are dammarane-type saponins containing an aglycone triterpene moiety.…”

Section: Fig 3 -Gp Reduces E Coli-mediated Induction Of Il-6 and Ilmentioning

confidence: 99%

Gynostemma pentaphyllum exhibits anti-inflammatory properties and modulates antimicrobial peptide expression in the urinary bladder

Lüthje

Lokman

Sandström

et al. 2015

Journal of Functional Foods

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Bioactive dammarane-type triterpenoids derived from the acid hydrolysate of Gynostemma pentaphyllum saponins

Cited by 34 publications

References 22 publications

Mechanism of action of gypenosides on type 2 diabetes and non-alcoholic fatty liver disease in rats

Mechanism of action of gypenosides on type 2 diabetes and non-alcoholic fatty liver disease in rats

Hypolipidemic mechanism of gypenosides via inhibition of pancreatic lipase and reduction in cholesterol micellar solubility

Gynostemma pentaphyllum exhibits anti-inflammatory properties and modulates antimicrobial peptide expression in the urinary bladder

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