Bioactive formulations prepared from fruiting bodies and submerged culture mycelia of the Brazilian edible mushroom Pleurotus ostreatoroseus Singer

Abstract

a Pleurotus ostreatoroseus is a Brazilian edible mushroom whose chemical characterization and bioactivity still remain underexplored. In this study, the hydrophilic and lipophilic compounds as well as the antioxidant, anti-inflammatory and antimicrobial activities of formulations (ethanol extracts) prepared with its fruiting bodies and submerged culture mycelia were compared. The bioactive formulations contain at least five free sugars, four organic acids, four phenolic compounds and two tocopherols. The fruiting body-based formulation revealed higher reducing power, DPPH scavenging activity, β-carotene bleaching inhibition and lipid peroxidation inhibition in brain homogenates than the mycelium-based preparation, as well as higher anti-inflammatory and antimicrobial activities. The absence of hepatotoxicity was confirmed in porcine liver primary cells. These functional responses can be related to the levels of bioactive components including phenolic acids, organic acids and tocopherols.

Bioactive characterization of Persea americana Mill. by-products: A rich source of inherent antioxidants

“…The minimum inhibitory (MIC), minimum bactericidal concentrations (MBC) and minimum fungicidal concentrations (MFC) were determined by methodologies, described by (Gomes-Correa et al, 2015). The minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations were determined by the microdilution method.…”

Effects of in vitro digestion and in vitro colonic fermentation on stability and functional properties of yerba mate (Ilex paraguariensis A. St. Hil.) beverages

“…Standard curves were constructed with trolox (r 2 = 0.99) and the results were expressed as mmol trolox equivalents (TE)/ mg lyophilisate material. The DPPH and the ABTS assays were conducted as described previously (Correa et al, 2015). The percentage of DPPH and ABTS discoloration was calculated using the following equation:…”

“…Inhibition of the production of thiobarbituric acid reactive substances (TBARS) was evaluated essentially as described by Correa et al (2015), except that rat brains instead of porcine brains were used as the lipid source. The color intensity of the malondialdehyde-thiobarbituric acid (MDA-TBA) was measured at the wavelength of 532 nm.…”

Effects of Carbon Sources and Time of Cultivation on the Antimicrobial Activities of Intra and Extracellular Extracts of Pleurotus Pulmonarius Cultured in Submerged Conditions

“…Furthermore, an antimicrobial activity was also reported for both the basidioma and the mycelium of various species of the genus such as P. nebrodensis and P. eryngii (Schillacietal, 2013), P. djamor (Dharmaraj et al, 2014) P. ostreatus (Younis et al, 2015), P. ostreatoroseus (Corrêa et al, 2015) and P. pulmonarius (Adebayo et al, 2012).…”