Bioactive hydrogel encapsulated dual-gene engineered nucleus pulposus stem cells towards intervertebral disc tissue repair

Ye, Yuchen; Xu, Panpan; Li, Cai; Jin, Shichang; Hu, Jie; Fang, Yuekun; Xu, Gang; Han, Zhongbing; Wu, Nan; Jiang, Pan; Bao, Zhengqi; Zhou, Pinghui; Zhang, Changchun

doi:10.1016/j.cej.2022.139717

Cited by 10 publications

(5 citation statements)

References 69 publications

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“… The morphology and ECM expression of NP MSCs following gene transfection ( Ye et al, 2023 ). (A) Observations of the morphological characteristics of NP-MSCs following transfection within the hydrogel.…”

Section: Therapeutic Factorsmentioning

confidence: 99%

“…This strategy based on delivering MSCs into NP and differentiating them into NP-like cells has been observed to inhibit and reverse IDD in both rabbit and dog models, providing evidence and hope for treating IDD with MSCs. With the increasing maturity of genetic engineering technology, Ye et al (2023) further utilized lentivirus-mediated insulin-like growth factor-1 and TGF-β3 dual gene transfection to effectively promote the proliferation and differentiation of NP MSCs into NP-like cells in vitro, increasing the effectiveness of MSCs in IDD treatment and proving the potential therapeutic value of genetic technology in IVD regeneration (Figure 2). Recently, Adipose-derived stem cells (ADSCs) have also played a role in treating IDD.…”

Section: Mesenchymal Stem Cells-based Idd Regenerationmentioning

confidence: 99%

“…With the maturity of genetic technology, various nucleic acids, such as plasmid DNA (pDNA), siRNAs, miRNAs, and circular RNA (circRNA), have been found to have different mechanisms of action and can regulate the level of cell gene expression. In recent years, The morphology and ECM expression of NP MSCs following gene transfection (Ye et al, 2023). Frontiers in Cell and Developmental Biology frontiersin.org gene therapy that directly introduces these nucleic acids into IVD through delivery systems has also shown promise for treating IDD.…”

Section: Genesmentioning

confidence: 99%

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Therapeutic factors and biomaterial-based delivery tools for degenerative intervertebral disc repair

Song,

Guo,

et al. 2024

Front. Cell Dev. Biol.

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Intervertebral disc degeneration (IDD) is the main cause of low back pain (LBP), which significantly impacts global wellbeing and contributes to global productivity declines. Conventional treatment approaches, encompassing conservative and surgical interventions, merely serve to postpone the advancement of IDD without offering a fundamental reversal. Consequently, there is an urgent demand for an effective approach to prevent the progression of IDD. Recent investigations focusing on the treatment of IDD utilizing diverse bioactive substances integrated within various biomaterials have exhibited promising outcomes. Various bioactive substances, encompassing conventional small molecule drugs, small molecule nucleic acids, and cell therapies, exhibit distinct capacities for repairing IDD. Additionally, various biological material delivery systems, such as nano micelles, microspheres, and hydrogels, possess diverse biological and release characteristics. Consequently, these diverse materials and drugs hold promise for advancing the treatment of IDD. This article aims to provide a concise overview of the IDD process and investigate the research advancements in biomaterials and bioactive substances for IDD treatment, delving into their mechanisms.

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Section: Therapeutic Factorsmentioning

confidence: 99%

Section: Mesenchymal Stem Cells-based Idd Regenerationmentioning

confidence: 99%

Section: Genesmentioning

confidence: 99%

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Therapeutic factors and biomaterial-based delivery tools for degenerative intervertebral disc repair

Song,

Guo,

et al. 2024

Front. Cell Dev. Biol.

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“…A bicomponent polymer network (BCN) hydrogel, constituted of GelMA and HAMA, was formulated as a vehicle to deliver genetically engineered NPMSCs to targeted tissues (as depicted in figure 10(i)). Incorporated with NPMSCs overexpressing IGF-1 and TGF-β3, BCN hydrogels effectively enhanced the expression of ECM and differentiation of NP [173].…”

Section: Intervertebral Disc Tissue Engineeringmentioning

confidence: 99%

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Pramanik,

Alhomrani,

Alamri

et al. 2024

Biomed. Mater.

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Gelatin methacryloyl (GelMA) hydrogels have gained significant recognition as versatile biomaterials in the biomedical domain. GelMA hydrogels emulate vital characteristics of the innate extracellular matrix (ECM) by integrating cell-adhering and matrix metalloproteinase-responsive peptide motifs. These features enable cellular proliferation and spreading within GelMA-based hydrogel scaffolds. Moreover, GelMA displays flexibility in processing, as it experiences crosslinking when exposed to light irradiation, supporting the development of hydrogels with adjustable mechanical characteristics. The drug delivery landscape has been reshaped by GelMA hydrogels, offering a favorable platform for the controlled and sustained release of therapeutic actives. The tunable physicochemical characteristics of GelMA enable precise modulation of the kinetics of drug release, ensuring optimal therapeutic effectiveness. In tissue engineering, GelMA hydrogels perform an essential role in the design of the scaffold, providing a biomimetic environment conducive to cell adhesion, proliferation, and differentiation. Incorporating GelMA in three-dimensional printing further improves its applicability in drug delivery and developing complicated tissue constructs with spatial precision. Wound healing applications showcase GelMA hydrogels as bioactive dressings, fostering a conducive microenvironment for tissue regeneration. The inherent biocompatibility and tunable mechanical characteristics of GelMA provide its efficiency in the closure of wounds and tissue repair.GelMA hydrogels stand at the forefront of biomedical innovation, offering a versatile platform for addressing diverse challenges in drug delivery, tissue engineering, and wound healing. This review provides a comprehensive overview, fostering an in-depth understanding of GelMA hydrogel’s potential impact on progressing biomedical sciences.

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“…Intervertebral disc disease caused by trauma, degeneration, protrusion, and other factors can cause low back pain, which seriously affects the quality of life of patients. As an ideal treatment for disc diseases, tissue engineering has become a hot topic at home and abroad. − Huang et al successfully prepared a decellularized intervertebral disc scaffold. Although the decellularized scaffold only tested the cell behavior in a short period of time and could not reflect the long-term situation, it still laid the foundation for the creation of a better decellularized scaffold in the future …”

Section: The Application Of Decellularized Scaffolds In Tissue Engine...mentioning

confidence: 99%

Biomedical Application of Decellularized Scaffolds

Zhang,

Gao,

Jiang

et al. 2023

ACS Appl. Bio Mater.

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Bioactive hydrogel encapsulated dual-gene engineered nucleus pulposus stem cells towards intervertebral disc tissue repair

Cited by 10 publications

References 69 publications

Therapeutic factors and biomaterial-based delivery tools for degenerative intervertebral disc repair

Therapeutic factors and biomaterial-based delivery tools for degenerative intervertebral disc repair

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Biomedical Application of Decellularized Scaffolds

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