Bioactive lipids regulate Trypanosoma cruzi development

Chagas-Lima, Alessandra C.; Pereira, Miria G.; Fampa, Patrı́cia; Lima, Michele S.; Kluck, George Eduardo Gabriel; Atella, Geórgia C.

doi:10.1007/s00436-019-06331-9

Cited by 9 publications

(4 citation statements)

References 50 publications

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“…For example, T. cruzi requires inositol for many of their membrane components, such as glycoinositolphospholipids, and utilizes transporters to acquire it for their synthesis ( Einicker-Lamas et al, 2007 ). Interestingly, the blood meal possesses signaling lipids, including oleic acid, LPC, and lysophosphatidic acid that promote the differentiation and proliferation of these parasites in triatomines ( Wainszelbaum et al, 2003 ; Chagas-Lima et al, 2019 ). If epimastigotes internalize excess lipids, they store them in specialized lipid organelles for later use ( Pereira et al, 2018 ).…”

Section: Lipid Scavenging By Extracellular Pathogensmentioning

confidence: 99%

Lipid hijacking: A unifying theme in vector-borne diseases

O’Neal

Butler

Rolandelli

et al. 2020

eLife

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Vector-borne illnesses comprise a significant portion of human maladies, representing 17% of global infections. Transmission of vector-borne pathogens to mammals primarily occurs by hematophagous arthropods. It is speculated that blood may provide a unique environment that aids in the replication and pathogenesis of these microbes. Lipids and their derivatives are one component enriched in blood and are essential for microbial survival. For instance, the malarial parasite Plasmodium falciparum and the Lyme disease spirochete Borrelia burgdorferi, among others, have been shown to scavenge and manipulate host lipids for structural support, metabolism, replication, immune evasion, and disease severity. In this Review, we will explore the importance of lipid hijacking for the growth and persistence of these microbes in both mammalian hosts and arthropod vectors.

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Section: Lipid Scavenging By Extracellular Pathogensmentioning

confidence: 99%

Lipid hijacking: A unifying theme in vector-borne diseases

O’Neal

Butler

Rolandelli

et al. 2020

eLife

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“…Other factors have been shown to impact the metacyclogenesis rate either in vivo and/or in vitro, such as the trypanosome redox state [35], the temperature in the insect vector [36] and the regulation of autophagy [37, 38]. One other important factor which determines the success of cell differentiation is the exposure to different substrates [3, 5, 39, 40]. Some substrates such as the amino acids Pro, Asn and Gln are ‘pro-metacyclogenic’, supporting cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Branched chain amino acids modulate the proteomic profile of Pro-induced differentiation for the infective stages ofTrypanosoma cruzi

de Freitas Nascimento,

Damasceno,

Marsicobettre

et al. 2024

Preprint

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Trypanosoma cruzi, the causative agent of Chagas disease, undergoes a complex life cycle involving triatomine insects as vectors and mammals. The differentiation of epimastigote forms into metacyclic trypomastigotes within the insect vector is crucial for the parasite's life cycle progression. Factors influencing this process, including temperature, pH, and nutritional stress, along with specific metabolite availability, play a pivotal role. Amino acids like Pro, His, and Gln support cell differentiation, while branched-chain amino acids (BCAAs) inhibit it. Interestingly, combining the pro-metacyclogenic amino acid Pro with one of the anti-metacyclogenic BCAAs results in viable metacyclics with significantly reduced infectivity. To explore the characteristics of metacyclic parasites differentiated in the presence of BCAAs, proteomics analyses were conducted. Metacyclics obtained in triatomine artificial urine (TAU) supplemented with Pro alone and in combination with Leu, Ile, or Val were compared. The analyses revealed differential regulation of 40 proteins in TAU-Pro-Leu, 131 in TAU-Pro-Ile, and 179 in TAU-Pro-Val, as compared to metacyclics from TAU-Pro. Among these, 22%, 11%, and 13% of the proteins were associated with metabolic processes, respectively. Notably, enzymes related to glycolysis and the tricarboxylic acid (TCA) cycle were reduced in metacyclics with Pro-BCAAs, while enzymes involved in amino acid and purine metabolic pathways were increased. Furthermore, metacyclics with Pro-Ile and Pro-Val exhibited elevated enzymes linked to lipid and redox metabolism. These findings suggest that the presence of BCAAs can reshape the metabolism of metacyclics, contributing to the observed reduction in infectivity in these parasites.

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“…LPA is another member of LPLs that was upregulated 1.82 times at 12 hpi, including two upregulated LPG lipid species. LPA is produced from LPC by activation of platelets, neuronal cells, and adipocytes in humans and has high specificity to receptors coupled to G proteins, as well as promotes the proliferation of Trypanosoma cruzi ( Chagas-Lima et al., 2019 ). Schistosomal-derived LPC can trigger M2 polarization of macrophages and activates human eosinophils, producing pro-inflammatory and immunoregulatory mediators ( Assunção et al., 2017 ; Magalhães et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection

Zou

Elsheikha

et al. 2022

Front. Cell. Infect. Microbiol.

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A global lipidomic analysis using liquid chromatography–tandem mass spectrometry was performed on the liver of beagle dogs infected with Toxocara canis to profile hepatic lipid species at 12 h post-infection (hpi), 24 hpi, and 36 days post-infection (dpi). This analysis identified six categories and 42 subclasses of lipids, including 173, 64, and 116 differentially abundant lipid species at 12 hpi, 24 hpi, and 36 dpi, respectively. Many of the identified lysophospholipids, such as lysophosphatidylglycerol, lysophosphatidylserine, and lysophosphatidylcholine, may contribute to the migration and development of T. canis during the early infection stage. Pathway analysis revealed significant alterations of several immune-inflammatory pathways, such as the B-cell receptor signaling pathway, the NF-kappa B signaling pathway, and the C-type lectin receptor signaling pathway at 12 and 24 hpi. These findings demonstrate the value of lipidomic profiling in revealing the extent of changes in the composition and abundance of hepatic lipidome caused by T. canis infection and their relevance to the pathophysiology of toxocariasis in beagle dogs.

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Bioactive lipids regulate Trypanosoma cruzi development

Cited by 9 publications

References 50 publications

Lipid hijacking: A unifying theme in vector-borne diseases

Lipid hijacking: A unifying theme in vector-borne diseases

Branched chain amino acids modulate the proteomic profile of Pro-induced differentiation for the infective stages ofTrypanosoma cruzi

Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection

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