Bioactive Minor Egg Components

Gautron, Joël; Guyot, Nicolas; Brionne, Aurélien; Réhault-Godbert, Sophie

doi:10.1039/9781788013833-00259

Cited by 9 publications

(10 citation statements)

References 153 publications

(145 reference statements)

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“…It represents the aqueous environment, in which sperm is exposed during its ascent from the vagina to the infundibulum. UF has been widely investigated over the past few years in relation to egg formation (Gautron, Guyot, Brionne, & Réhault‐Godbert, 2019). It is a selective transudate of serum, which also contains locally produced factors, mainly related to the metabolic activity of uterine epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

“…It contains several key proteins involved in the eggshell mineralization process that occurs from 5 hr after ovulation (Marie et al, 2015; Sun, Xu, & Yang, 2013). It is also composed of bactericidal factors, providing an aseptic package for embryo development (Gautron et al, 2007; Gautron et al, 2019; Silphaduang, Hincke, Nys, & Mine, 2006; Wellman‐Labadie, Lakshminarayanan, & Hincke, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Avian uterine fluid proteome: Exosomes and biological processes potentially involved in sperm survival

Riou

Brionne

Cordeiro

et al. 2020

Molecular Reproduction Devel

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Uterine fluid is an aqueous milieu to which sperm are exposed during their storage and ascent. In this study, a bottom-up proteomic strategy and bioinformatic analysis of hen uterine fluid was performed to improve the understanding of this fluid and its potential role in sperm survival mechanisms. The proteomic data were submitted to ProteomeXchange. Among the 913 proteins identified, 160 are known to be secreted and 640 are referenced in exosomes databases. We isolated exosomes from the avian uterine fluid, analyzed them using electron microscopy, and targeted several exosomes markers (ANXA1/2/4/5, VCP, HSP90A, HSPA8, PARK7, and MDH1) using immunoblotting. Electron microscopy and immunohistochemistry were also used to analyze uterovaginal junctions for the exosomal proteins ANXA4, VCP, and PARK7. Exosomes were observed both at the surface epithelium and inside sperm storage tubules. Our data were compared with two previously published studies on proteomic of hen uterine fluid, and with one study describing the proteomic content of rooster seminal plasma and sperm. In conclusion, we demonstrated for the first time that avian uterine fluid contains exosomes. These may play a key role in preserving sperm functions within the female genital tract. Their presence in the sperm storage tubules may represent an important mechanism regarding interaction between the female genital tract and sperm. K E Y W O R D S avian, exosomes, proteome, sperm, uterine fluid

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Avian uterine fluid proteome: Exosomes and biological processes potentially involved in sperm survival

Riou

Brionne

Cordeiro

et al. 2020

Molecular Reproduction Devel

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“…ESM contains an abundance of antimicrobial, immunomodulatory, and other bioactive proteins, peptides, amino acids, collagen-like proteins, enzymes, and glycosaminoglycans (Yamauchi et al, 2013 ; Yoo et al, 2014 ; Benson et al, 2016 ; Jensen et al, 2016 ; Ahmed et al, 2017 , 2019a , b ; Niu et al, 2017 ; Dubourdieu, 2019 ; Gautron et al, 2019 ; Zhao et al, 2019b ; Zhu, 2020 ). Each of them can have a pronounced influence on wound healing as a biologically active compound.…”

Section: Eggshell In Therapeuticsmentioning

confidence: 99%

State-of-the-Art of Eggshell Waste in Materials Science: Recent Advances in Catalysis, Pharmaceutical Applications, and Mechanochemistry

Baláž

Болдырева

Rybin

et al. 2021

Front. Bioeng. Biotechnol.

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Eggshell waste is among the most abundant waste materials coming from food processing technologies. Despite the unique properties that both its components (eggshell, ES, and eggshell membrane, ESM) possess, it is very often discarded without further use. This review article aims to summarize the recent reports utilizing eggshell waste for very diverse purposes, stressing the need to use a mechanochemical approach to broaden its applications. The most studied field with regards to the potential use of eggshell waste is catalysis. Upon proper treatment, it can be used for turning waste oils into biodiesel and moreover, the catalytic effect of eggshell-based material in organic synthesis is also very beneficial. In inorganic chemistry, the eggshell membrane is very often used as a templating agent for nanoparticles production. Such composites are suitable for application in photocatalysis. These bionanocomposites are also capable of heavy metal ions reduction and can be also used for the ozonation process. The eggshell and its membrane are applicable in electrochemistry as well. Due to the high protein content and the presence of functional groups on the surface, ESM can be easily converted to a high-performance electrode material. Finally, both ES and ESM are suitable for medical applications, as the former can be used as an inexpensive Ca2+ source for the development of medications, particles for drug delivery, organic matrix/mineral nanocomposites as potential tissue scaffolds, food supplements and the latter for the treatment of joint diseases, in reparative medicine and vascular graft producing. For the majority of the above-mentioned applications, the pretreatment of the eggshell waste is necessary. Among other options, the mechanochemical pretreatment has found an inevitable place. Since the publication of the last review paper devoted to the mechanochemical treatment of eggshell waste, a few new works have appeared, which are reviewed here to underline the sustainable character of the proposed methodology. The mechanochemical treatment of eggshell is capable of producing the nanoscale material which can be further used for bioceramics synthesis, dehalogenation processes, wastewater treatment, preparation of hydrophobic filters, lithium-ion batteries, dental materials, and in the building industry as cement.

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“…However, in other groundnesting species, such as ostrich, chicken, turkey, etc., the eggshell ultrastructure is columnar. The ultrastructure, polymorph and nucleation/growth of calcium carbonate crystals are controlled by a specific macromolecular toolkit, the organic matrix (OM) (Chien et al, 2008;Hernandez-Hernandez et al, 2008a,b;Zhao et al, 2013;Gautron et al, 2019Gautron et al, , 2021. The bird eggshell OM represents 3.5% of the total shell weight including shell membranes.…”

Section: Avian Eggshell: An Exceptional Vertebrate Calcium Carbonate Biomineralmentioning

confidence: 99%

Evolution of the Avian Eggshell Biomineralization Protein Toolkit – New Insights From Multi-Omics

et al. 2021

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The avian eggshell is a remarkable biomineral, which is essential for avian reproduction; its properties permit embryonic development in the desiccating terrestrial environment, and moreover, are critically important to preserve unfertilized egg quality for human consumption. This calcium carbonate (CaCO3) bioceramic is made of 95% calcite and 3.5% organic matrix; it protects the egg contents against microbial penetration and mechanical damage, allows gaseous exchange, and provides calcium for development of the embryonic skeleton. In vertebrates, eggshell occurs in the Sauropsida and in a lesser extent in Mammalia taxa; avian eggshell calcification is one of the fastest known CaCO3 biomineralization processes, and results in a material with excellent mechanical properties. Thus, its study has triggered a strong interest from the researcher community. The investigation of eggshell biomineralization in birds over the past decades has led to detailed characterization of its protein and mineral constituents. Recently, our understanding of this process has been significantly improved using high-throughput technologies (i.e., proteomics, transcriptomics, genomics, and bioinformatics). Presently, more or less complete eggshell proteomes are available for nine birds, and therefore, key proteins that comprise the eggshell biomineralization toolkit are beginning to be identified. In this article, we review current knowledge on organic matrix components from calcified eggshell. We use these data to analyze the evolution of selected matrix proteins and underline their role in the biological toolkit required for eggshell calcification in avian species. Amongst the panel of eggshell-associated proteins, key functional domains are present such as calcium-binding, vesicle-binding and protein-binding. These technical advances, combined with progress in mineral ultrastructure analyses, have opened the way for new hypotheses of mineral nucleation and crystal growth in formation of the avian eggshell, including transfer of amorphous CaCO3 in vesicles from uterine cells to the eggshell mineralization site. The enrichment of multi-omics datasets for bird species is critical to understand the evolutionary context for development of CaCO3 biomineralization in metazoans, leading to the acquisition of the robust eggshell in birds (and formerly dinosaurs).

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Bioactive Minor Egg Components

Cited by 9 publications

References 153 publications

Avian uterine fluid proteome: Exosomes and biological processes potentially involved in sperm survival

Avian uterine fluid proteome: Exosomes and biological processes potentially involved in sperm survival

State-of-the-Art of Eggshell Waste in Materials Science: Recent Advances in Catalysis, Pharmaceutical Applications, and Mechanochemistry

Evolution of the Avian Eggshell Biomineralization Protein Toolkit – New Insights From Multi-Omics

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