Bioactive Organocopper Compound from Pseudomonas aeruginosa Inhibits the Growth of Xanthomonas citri subsp. citri

Oliveira, Admilton Gonçalves de; Spago, Flávia Regina; Simionato, Ane Stéfano; Navarro, Miguel Octavio Pérez; Silva, Caroline S. da; Barazetti, André Riedi; Cely, Martha Viviana Torres; Tischer, César Augusto; Martin, Juca Abramo Barrera San; Andrade, Célia Guadalupe Tardeli de Jesus; Novello, Cláudio Roberto; Mello, João Carlos Palazzo de; Andrade, Galdino

doi:10.3389/fmicb.2016.00113

Cited by 54 publications

(40 citation statements)

References 45 publications

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“…Scanning electron microscopy (SEM) was performed based on the protocol described by Oliveira [34]. C. krusei ATCC 6258 (2-3×10 3 cells/mL in RPMI-1640 medium) was exposed to LMM11 at the concentrations of 16 μg/mL, 32 μg/mL, 64 μg/mL and incubated in 24-well plates at 35˚C for 24 h. The cells were harvested, washed twice with PBS and fixed by immersion in 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1 M sodium cacodylate buffer.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Promising antifungal activity of new oxadiazole against Candida krusei

et al. 2020

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Candida krusei is one of the most common agents of invasive candidiasis and candidemia worldwide, leading to high morbidity and mortality rates. This species has become a problem due to its intrinsic resistance and reduced susceptibility to azoles and polyenes. Moreover, the number of antifungal drugs available for candidiasis treatment is limited, demonstrating the urgent need for the discovery of novel alternative therapies. In this work, the in vivo and in vitro activities of a new oxadiazole (LMM11) were evaluated against C. krusei. The minimum inhibitory concentration ranged from 32 to 64 μg/mL with a significant reduction in the colony forming unit (CFU) count (~3 log 10). LMM11 showed fungicidal effect, similar to amphotericin, reducing the viable cell number (>99.9%) in the time-kill curve. Yeast cells presented morphological alterations and inactive metabolism when treated with LMM11. This compound was also effective in decreasing C. krusei replication inside and outside macrophages. A synergistic effect between fluconazole and LMM11 was observed. In vivo treatment with the new oxadiazole led to a significant reduction in CFU (0.85 log 10). Furthermore, histopathological analysis of the treated group exhibited a reduction in the inflammatory area. Taken together, these results indicate that LMM11 is a promising candidate for the development of a new antifungal agent for the treatment of infections caused by resistant Candida species such as C. krusei.

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Section: Scanning Electron Microscopymentioning

confidence: 99%

Promising antifungal activity of new oxadiazole against Candida krusei

et al. 2020

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“…Many pharmaceutically important antibiotics have been identified in the past from this bioresource, e.g., vancomycin produced by Streptomyces orientalis isolated from a soil sample from Borneo ( Griffith, 1981 ), kanamycin produced by a soil bacterium Streptomyces kanamyceticus ( Umezawa et al, 1957 ), and erythromycin first isolated in 1952 from the soil bacterium Saccharopolyspora erythraea ( Staunton and Wilkinson, 1997 ). Bacterial genera reported so far as a bioresource with a high chance to detect compounds of interest are Actinomycetes ( Hotam et al, 2013 ; Tiwari and Gupta, 2013 ), Bacilli ( Sumi et al, 2015 ), and Pseudomonads ( Mukherjee et al, 2014 ; de Oliveira et al, 2016 ). In the present project so far unexplored arid sampling sites of Southern Tunisia were investigated, since the arid environment results in high competition between organisms.…”

Section: Introductionmentioning

confidence: 99%

Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives

et al. 2018

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Arid regions show relatively fewer species in comparison to better-watered biomes, but the competition for the few nutrients is very distinct. Here, in total 373 bacterial strains were isolated from rhizospheric soils obtained from three different sampling sites in Tunisia. Their potential for the production of antimicrobial compounds was evaluated. Bacterial strains, showing antibacterial activity against pathogenic bacteria, were isolated from all three sites, one strain from the Bou-Hedma national park, 15 strains from Chott-Djerid, and 13 strains from Matmata, respectively. The dominant genus was Bacillus, with 27 out of 29 strains. Most interestingly, 93% of the isolates showed activity against Gram-positive and Gram-negative test bacteria. Strain Bacillus sp. M21, harboring high inhibitory potential, even against clinical isolates of Gram-negative bacteria, was analyzed in detail to enable purification and identification of the bioactive compound responsible for its bioactivity. Subsequent HPLC-MS and NMR analyses resulted in the identification of 1-acetyl-β-carboline as active component. Furthermore, fungicides of the bacillomycin and fengycin group, which in addition show antibiotic effects, were identified. This work highlights the high potential of the arid-adapted strains for the biosynthesis of specialized metabolites and suggest further investigation of extreme environments, since they constitute a promising bioresource of biologically active compounds.

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“…The cells were recovered by centrifugation in a Jouan CR3 equipment (9,000 rpm/20 min, 4°C), resuspended in TRIZOL (INVITROGEN) and kept at −80°C. The confirmation of the antibiotic activity of the supernatants was confirmed as described elsewhere (De Oliveira et al, 2016 ). Both cultures were performed in biological triplicate (three samples for each condition, corresponding to three experiments separated spatially and temporally).…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, the same strain produced a Phenazine-1-Carboxylic Acid (even if in low quantity) which shown antifungal potential against Botrytis cinerea (Simionato et al, 2017 ). Apparently, the copper chloride induces the production of this compounds, since no antimicrobial activity can be detected in the supernatant of P. aeruginosa strain LV cultured in its absence (De Oliveira et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

New Insights about Antibiotic Production by Pseudomonas aeruginosa: A Gene Expression Analysis

et al. 2017

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The bacterial resistance for antibiotics is one of the most important problems in public health and only a small number of new products are in development. Antagonistic microorganisms from soil are a promising source of new candidate molecules. Products of secondary metabolism confer adaptive advantages for their producer, in the competition for nutrients in the microbial community. The biosynthesis process of compounds with antibiotic activity is the key to optimize their production and the transcriptomic study of microorganisms is of great benefit for the discovery of these metabolic pathways. Pseudomonas aeruginosa LV strain growing in the presence of copper chloride produces a bioactive organometallic compound, which has a potent antimicrobial activity against various microorganisms. The objective of this study was to verify overexpressed genes and evaluate their relation to the organometallic biosynthesis in this microorganism. P. aeruginosa LV strain was cultured in presence and absence of copper chloride. Two methods were used for transcriptomic analysis, genome reference-guided assembly and de novo assembly. The genome referenced analysis identified nine upregulated genes when bacteria were exposed to copper chloride, while the De Novo Assembly identified 12 upregulated genes. Nineteen genes can be related to an increased microbial metabolism for the extrusion process of exceeding intracellular copper. Two important genes are related to the biosynthesis of phenazine and tetrapyrroles compounds, which can be involved in the bioremediation of intracellular copper and we suggesting that may involve in the biosynthesis of the organometallic compound. Additional studies are being carried out to further prove the function of the described genes and relate them to the biosynthetic pathway of the organometallic compound.

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Bioactive Organocopper Compound from Pseudomonas aeruginosa Inhibits the Growth of Xanthomonas citri subsp. citri

Cited by 54 publications

References 45 publications

Promising antifungal activity of new oxadiazole against Candida krusei

Promising antifungal activity of new oxadiazole against Candida krusei

Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives

New Insights about Antibiotic Production by Pseudomonas aeruginosa: A Gene Expression Analysis

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