Bioactive potential and RP-HPLC detection of phenolics and alkaloids (lycorine and galanthamine) from ultrasonic-assisted extracts of Crinum roots

Lekhak, Manoj M.; Patel, Suraj B.; Otari, Shreedhar S.; Lekhak, U.M.; Ghane, Savaliram G.

doi:10.1016/j.sajb.2021.07.024

Cited by 11 publications

(5 citation statements)

References 54 publications

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“…Numerous secondary metabolites including stigmasterol also demonstrated potential biological functions and play a significant role in the food and pharmaceutical industries. Different plant parts have medicinal potential due to the presence of secondary metabolites such as phenolics, flavonoids, alkaloids, terpenoids, and sterols ( Otari et al., 2022 ; Patel et al., 2022 ; Ghane et al., 2018 ; Lekhak et al., 2021 ). Many bioactive substances appear to have a variety of health benefits; thus, the demand for them in the food and pharmaceutical industries is high ( Bhattacharyya et al., 2022 ; Otari et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Micropropagation and elicited production of triterpenoid saponin glycosides and stigmasterol via precursor and elicitor feeding in Bacopa floribunda (R.Br.) Wettst.—A potential nootropic herb

et al. 2023

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Bacopa floribunda (Family: Plantaginaceae) is used in folklore medicines for the management of cognitive dysfunction. It has nootropic, antiaging, anti-inflammatory, anti-cholinesterase, and antioxidant properties. We developed an efficient and reproducible protocol for in vitro propagation of B. floribunda using the nodal explants. We assessed the effects of Murashige and Skoog (MS) medium fortified with various plant growth regulatory substances (PGRs), a precursor, and elicitors and their optimal combinations on regeneration and production of total saponins, triterpenoid saponin glycosides (bacoside A3, bacopaside X, bacopaside II, and bacosaponin C), and stigmasterol content in in vitro grown biomass of B. floribunda. The growth of the shoots and roots was stimulated by MS + 2.0 mg/l BAP + 2.0 mg/l KIN and MS + 0.5 mg/l IAA + 0.5 mg/l IBA + 1.0 mg/l NAA, respectively. After 10 weeks of acclimatization, plantlets of B. floribunda had a survival rate of 95%. The highest total saponin content (35.95 ± 0.022 mg DE/g DW) was noted in the treatment of MS + 2.0 mg/l BAP + 1.5 μM SQ. Similarly, total triterpenoid saponin glycosides and stigmasterol were found maximum in biomass derived from MS + 2.0 mg/l BAP + 1.5 μM SQ and MS + 2.0 mg/l BAP, respectively. At the same treatments, bacoside A3 (1.01 ± 0.195 mg/g DW), bacopaside II (43.62 ± 0.657 mg/g DW), bacopaside X (1.23 ± 0.570 mg/g DW), bacosaponin C (0.19 ± 0.195 mg/g DW), and stigmasterol (7.69 ± 0.102 mg/g DW) were reported. The present findings will help to highlight B. floribunda as a potent memory-enhancing herb, and in future also, it could be a potential substitute to B. monnieri. The current work is the first to describe the micropropagation and the elicited production of bioactive metabolites from the in vitro grown biomass of B. floribunda. In addition, further research is needed on production of bioactives, their pharmacological effects, and the elicited production using callus, cell suspension, and hairy root cultures.

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Section: Discussionmentioning

confidence: 99%

Micropropagation and elicited production of triterpenoid saponin glycosides and stigmasterol via precursor and elicitor feeding in Bacopa floribunda (R.Br.) Wettst.—A potential nootropic herb

et al. 2023

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“…HPLC analysis confirmed the presence of potent phenolics and phenylethanoid glycoside (verbascoside). Bioactive compounds found in plants also play important role in pharmaceutics and food industry, where some secondary metabolites showed potential biological activities ( Nescatelli et al, 2017 ; Stefanucci et al, 2018 ; Uysal et al, 2019 ; Lekhak et al, 2021 ; Mollica et al, 2021 ; Patel and Ghane, 2021 ). Ranade et al (2016) concluded that among phenolics, GA and CA were found the most common and abundant in leaves and stem of B. prionitis .…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Studies on Two Unexplored Endemic Medicinal Plants of India, Barleria terminalis and Calacanthus grandiflorus

et al. 2022

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Barleria terminalis Nees and Calacanthus grandiflorus (Dalzell) Radlk. are endemic medicinal plants of the Western Ghats of India. The aim of the present research work was to investigate phytochemical profile, potent bioactives using RP-HPLC, LC-MS and GC-MS and to evaluate their bioactivities. Acetone was found to be the best extraction medium for separating phytochemicals. Similarly, acetone and methanol extracts exhibited potential antioxidant properties. Ethanol extract of B. terminalis stem showed potent acetylcholinesterase (AChE) (89.10 ± 0.26%) inhibitory activity. Inhibition of α-amylase (36.96 ± 2.96%) activity was observed the best in ethanol extract of B. terminalis leaves and α-glucosidase inhibitory activity (94.33 ± 0.73%) in ethanol extract of C. grandiflorus stem. RP-HPLC analysis confirmed the presence of several phenolic compounds (gallic acid, hydroxybenzoic acid, vanillic acid, chlorogenic acid and coumaric acid) and phenylethanoid glycoside (verbascoside). The highest phenolics content were observed in B. terminalis (GA (4.17 ± 0.002), HBA (3.88 ± 0.001), VA (4.54 ± 0.001), CHLA (0.55 ± 0.004) mg/g DW, respectively). Similarly, LC-MS and GC-MS revealed the presence of phenolics, glycosides, terpenes, steroids, fatty acids, etc. Moreover, positive correlation between studied phytochemicals and antioxidants was observed in principal component analysis. Based on the present investigation, we conclude that B. terminalis and C. grandiflorus can be further explored for their active principles particularly, phenylethanoid glycosides and iridoids and their use in drug industry for pharmaceutical purposes.

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“…Previous studies have been intended to attempt the chemical synthesis of galanthamine, but industrial-scale production has not been achieved due to the complicated synthesis steps, harsh conditions, high costs, low yields and serious environmental pollution ( Zhang et al, 2019 ). A green and high yield extraction process for galanthamine is a hot research topic ( Tian et al, 2016 ; Akram et al, 2020 ; Lekhak et al, 2021 ). Galanthamine is unstable in air, and salt modification is generally adopted to improve its stability ( Liu, 2006 ), but studies on thermal stability and decomposition mechanism have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Analysis on the thermal decomposition kinetics and storage period of biomass-based lycorine galanthamine

2023

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As global ageing deepens and galanthamine is the preferred clinical drug for the treatment of mild to moderate Alzheimer’s disease, it will be valuable to examine the behaviour and mechanism of galanthamine’s thermal decomposition for its quality control, formulation process, evaluation of thermal stability, and expiry date in production. In order to study the pyrolysis of galanthamine hydrobromide with nitrogen as the carrier gas, a thermogravimetric-differential thermogravimetric technique (TG-DTG) was applied at a temperature rise rate of 10 K min−1 and a volume flow rate of 35 mL min−1. The apparent activation energy Ea and the prefactor A (Ea = 224.45 kJ mol−1 and lnA = 47.40) of the thermal decomposition reaction of galanthamine hydrobromide were calculated according to the multiple heating rate method (Kissinger and Ozawa) and the single heating rate method (Coats-Redfern and Achar), and the most probable mechanism function was derived, and then the storage period was inferred from Ea and E. A three-dimensional diffusion mechanism was suggested to control the thermal decomposition of galanthamine hydrobromide in accordance with the Jander equation, random nucleation and subsequent growth control, corresponding to the Mample one-way rule and the Avrami-Erofeev equation. As a result, the thermal decomposition temperature of galanthamine hydrobromide gradually increased with the rate of temperature rise. From Gaussian simulations and thermogravimetric data, galanthamine hydrobromide decomposed at the first stage (518.25–560.75 K) to release H2O, at the second stage (563.25–650.75 K) to generate CO, CO2, NH3 and other gases, and finally at the third stage (653.25–843.25 K) to release CO2. After 843.25 K, the residual molecular skeleton is cleaved to release CO2 and H2O. According to the Ea and A presenting in the first stage of thermal decomposition, it is assumed that the storage life of galanthamine hydrobromide at room temperature 298.15 K is 4–5 years.

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Bioactive potential and RP-HPLC detection of phenolics and alkaloids (lycorine and galanthamine) from ultrasonic-assisted extracts of Crinum roots

Cited by 11 publications

References 54 publications

Micropropagation and elicited production of triterpenoid saponin glycosides and stigmasterol via precursor and elicitor feeding in Bacopa floribunda (R.Br.) Wettst.—A potential nootropic herb

Micropropagation and elicited production of triterpenoid saponin glycosides and stigmasterol via precursor and elicitor feeding in Bacopa floribunda (R.Br.) Wettst.—A potential nootropic herb

Phytochemical Studies on Two Unexplored Endemic Medicinal Plants of India, Barleria terminalis and Calacanthus grandiflorus

Analysis on the thermal decomposition kinetics and storage period of biomass-based lycorine galanthamine

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