Bioactive Properties of Eremostachys macrophylla Montbr. &amp; Auch. Rhizomes Growing in Iran

Asgharian, Parina; Delazar, Abbas; Lotfipour, Farzaneh; Asnaashari, Solmaz

doi:10.15171/ps.2017.35

Cited by 8 publications

(4 citation statements)

References 24 publications

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“…24 According to the references, the total phenolic content and DPPH inhibition activity of the extracts are related. 25 Phenolic contents of extracts are responsible for the reduction of free radicals and exhibit chelating properties. 26 Our present study reveals that the total flavonoid and phenolic contents of the 20% fraction were higher in comparison to the other samples, responsible for their free radical scavenging activity.…”

Section: Resultsmentioning

confidence: 99%

Bioactivity Assays of Eryngium thyrsoideum; Focusing on Cytotoxic Effects, Antioxidant Activity, and Antimalarial Properties

et al. 2023

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Background: The genus Eryngium, Apiaceae is widely distributed in the world and used in traditional medicine for different therapeutic purposes. Eryngium thyrsoideum was investigated due to a lack of phytochemical studies on the probable bioactivities of its compounds. Methods: The aerial parts of the plant were extracted using n-hexane, dichloromethane, and methanol (MeOH) by the soxhlet method. The MeOH extract was exposed to C18 Sep-Pak fractionation by a step gradient of MeOH-H2 O. The essential oil was obtained by the Clevenger apparatus. Free-radical scavenging activity of the extracts and fractions was assessed using the DPPH-scavenging activity method. Total flavonoid content was determined by Aluminum chloride colorimetric and phenolic content was determined by using the Folin-Ciocalteu reagent method. α-Glucosidase inhibitory and antimalarial activity of extracts and essential oil and cytotoxic activity of essential oils was also established in this study. Results: The 20% solid phase extraction fraction demonstrated high antioxidant activity (0.13±0.01 mg/mL) and also indicated the most total flavonoid and total phenolic contents (474.20±23.11 and 27.90±0.55 mg/100 g) respectively, in comparison to other fractions. We observed a moderate β-Hematin Formation Assay inhibitory effect of the essential oil (10.37±0.02 mg/mL) and DCM extract (7.64±0.01 mg/mL). Low levels of α-glucosidase inhibition were observed in n-hexane extract (9.78±1.1%) and essential oil of aerial parts (2.7±1.1%). Cytotoxic activity for the essential oils is confirmed by representing respectively IC50 with 2.51 mcg/mL and 1.07 mcg/mL in 24 and 48 hours in MCF-7 cell lines. Conclusion: The presence of phenolic compounds and flavonoids in MeOH extract and fractions is effective on the antioxidant potency of E. thyrsoideum. Essential oil of this plant presented noticeable effects on human breast cancer cell lines.

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Section: Resultsmentioning

confidence: 99%

Bioactivity Assays of Eryngium thyrsoideum; Focusing on Cytotoxic Effects, Antioxidant Activity, and Antimalarial Properties

et al. 2023

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“…The free radical scavenging potential of EO was assessed on the basis of reduction of DPPH (Sigma-Aldrich) according to our previous works. 15,[23][24][25] DPPH (8 mg) was dissolved in chloroform (100 ml) to get a concentration of 80 mcg/ml. The various concentrations of the EO were prepared from stock solution (1mg/ml).…”

Section: Determination Of In-vitro Radical Scavenging Activitymentioning

confidence: 99%

“…6,7 With the exception of the reports on the effect of environmental factors on seed germination of L. vesicarium, 1 to the best of our knowledge since now, there are no scientific data available in the literatures on any volatile chemicals or bioactivity study on this species. Since part of our mission at the Pharmacognosy Department of Faculty of Pharmacy, Tabriz, is to discover plant non-volatile, [9][10][11][12][13][14][15][16] and volatile compositions, 17,18 which can be used as an anti-oxidant, [10][11][12][13][14][15][16][17] and anti-microbial agents, 9,11,12,15,17 volatile constituents as well as anti-microbial and antioxidant, properties of this plant were also evaluated. Other investigations on the non-volatile compositions of L. vesicarium are in progress.…”

Section: Introductionmentioning

confidence: 99%

GC-MS Analysis, Antioxidant and Antimicrobial Screening of Volatile Oil of Lepidium vesicarium

et al. 2018

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Background: Lepidium vesicarium (Cruciferae), one of the important medicinal plants with a long history of medicinal use. The current study was designed to evaluate the free radical scavenging and antimicrobial activities of the L. vesicarium EO as one of the Iranian plant species. Methods: The compositions of the EO from the aerial parts of L. vesicarium were analyzed by GC-MS and GC-FID. Furthermore, anti-oxidant and anti-microbial potentials were investigated via DPPH reagent and disk diffusion procedure, respectively. Results: A total of 18 compounds amounting 97.70% of the oil have been identified, while Benzyl cyanide (43.94%), Isothio cyanic acid (22.69%) and Benzyl isothio cyanate (20.69%) were the main constituents. The EO showed no activity against the free radicals and studied microbial strains (gram positive and gram negative and also fungi species). Conclusion: On the whole, the presence of cyanide derivatives in studied EO revealed the rational use of this plant in medicine. Comparing with other genesis of Lepidium, anti-oxidant and anti -microbial properties of L. vesicarium essential oil were not noticeable.

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“…The cells were kept in a moistened atmosphere of a 5% CO 2 (37°C). (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, USA) colorimetric assay was employed to determine the antiproliferative activity of medicinal plants (25). MTT was dissolved in phosphate buffered serum (5 mg/mL PBS).…”

Section: Mtt Assaymentioning

confidence: 99%

Biological and Phytochemical Screening of Eremostachys azerbaijanica Rech.f. Aerial Parts

Delazar

Asgharian

Asnaashari

2017

Jundishapur J Nat Pharm Prod

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Bioactive Properties of Eremostachys macrophylla Montbr. & Auch. Rhizomes Growing in Iran

Cited by 8 publications

References 24 publications

Bioactivity Assays of Eryngium thyrsoideum; Focusing on Cytotoxic Effects, Antioxidant Activity, and Antimalarial Properties

Bioactivity Assays of Eryngium thyrsoideum; Focusing on Cytotoxic Effects, Antioxidant Activity, and Antimalarial Properties

GC-MS Analysis, Antioxidant and Antimicrobial Screening of Volatile Oil of Lepidium vesicarium

Biological and Phytochemical Screening of Eremostachys azerbaijanica Rech.f. Aerial Parts

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