Bioactivities and chemical profiling comparison and metabolomic variations of polyphenolics and steroidal glycoalkaloids in different parts of Solanum nigrum L.

Abstract

IntroductionSolanum nigrum L. is a traditional medicinal herb and edible plant. Many studies provide evidence that S. nigrum L. is a nutritious vegetable. Polyphenols and steroidal glycoalkaloids are the main components.ObjectivesThis study aimed to systemically evaluate the phytochemical profile, quantification, and bioactivities of polyphenolics and glycoalkaloids in different parts of S. nigrum L.ResultsTotal polyphenols (TPC) and total glycoalkaloids (TGK) were determined using the Folin–Ciocalteu and acid dye colorimetric methods, respectively. A total of 55 polyphenolic constituents (including 22 phenolic acids and 33 flavonoids) and 24 steroidal glycoalkaloids were identified from different parts using ultrahigh‐performance liquid chromatography Q‐exactive high‐resolution mass spectrometry (UHPLC‐QE‐HRMS), of which 40 polyphenols (including 15 phenolic acids and 25 flavonoids) and one steroidal glycoalkaloid were characterised for the first time in S. nigrum L. Moreover, typical polyphenols and glycoalkaloids were determined using HPLC‐UV and HPLC–evaporative light‐scattering detector (ELSD), respectively. In addition, the TPC and TGK and their typical constituents were compared in different anatomical parts. Finally, the antioxidant capacities of polyphenolic extracts from different parts of S. nigrum L. were evaluated by ·OH, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging and ferric‐reducing antioxidant power (FRAP) assay in vitro. In addition, the antitumour effects of TGK from different parts of S. nigrum L. on the proliferation of PC‐3 cells were investigated using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. Polyphenolic and glycoalkaloid extracts from different parts of S. nigrum L. showed different antioxidant and cytotoxic capacities in vitro.ConclusionThis is the first study to systematically differentiate between polyphenolic and glycoalkaloid profiles from different parts of S. nigrum L.

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“…SGAs are a class of secondary metabolites produced by some plants in the Solanaceae family, such as tomatoes, potatoes, and eggplants. They are distributed in different botanical parts, which include the leaves, flowers, stems, roots, and unripe fruits [20]. Tomatine was firstly isolated by Fontaine et al, in tomato leaves and represents the main SGA isolated from the whole tomato plant [21].…”

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“…This significant discovery underscores the untapped potential of Solanum nigrum L. as a source of novel bioactive compounds, particularly its glycoalkaloids, urging further exploration for their promising anticancer properties. 62 …”