Bioactivity evaluations of leaf extract fractions from young barley grass and correlation with their phytochemical profiles

Abstract

Background: The pressed juice of Barley Grass (BG) has become very popular among people for various assumed benefits along with many testimonies of people who have been healed from various ailments such as anemia, cancer, GI problems by consuming BG. The aim of our research was to validate the claims of its medicinal values such as chemo-protective action, high anti-oxidants, RBC membrane stabilization activity, and toxicity level. Methods: Extracts of hexane, ethyl acetate and methanol were quantitatively estimated for total phenolic contents (TPC) and total flavonoid contents (TFC). The same extracts were assessed for their antioxidative potentials with the use of DPPH free radical scavenging assay followed by determination of HRBC membrane stabilization method, Brine Shrimp Lethality Assay (BSLA) and GC-MS analysis. Results: All the extracts showed high TPC and TFC along with the stronger correlation with the antioxidant activity of the extracts suggesting phenolics and flavonoids contents of the extract might be attributed to showing antioxidant activity. The methanolic and ethyl acetate extracts of the plant also showed remarkable antiinflammatory activity where methanolic extracts had the lowest EC50. During Brine Shrimp Lethality Assay, all extracts of BG were found to be bioactive and the degree of lethality was found to be concentration dependent. The GC-MS analysis of the methanolic extract of BG revealed 23 compounds which are reported to possess different biological activities. Conclusion: The study reveals the strong antioxidant and RBC membrane stabilization activity of BG. The Brine Shrimp Lethality Assay found extracts to be bioactive suggesting extracts as a promising candidate for plantderived anti-tumor compounds. Further, studies are needed to validate the data on cancer cell lines.

Chemical Composition and Protective Effect of Young Barley (Hordeum vulgare L.) Dietary Supplements Extracts on UV-Treated Human Skin Fibroblasts in In Vitro Studies

“…In an experiment conducted by Samsonowicz and Regulska (2018), aqueous extracts of barley seedlings were characterized by TPC values up to 10.7 mg GAE/g and ethanolic extracts up to 10.9 mg GAE/g [63]. A study by Panthi et al (2020) showed that the methanolic extract of young barley juice had the highest polyphenol content (82.56 mg GAE/g) [64].…”

Reinwardtia indica: phytochemical screening and evaluation of wound healing activity of the extracts in experimental model rats

“…It might be due to the presence of phytochemicals such as flavonoids, terpenoids, steroids, tannin, and phenolic compounds which were identified to promote wound healing activity mainly because of their antibacterial and antioxidant activity (10, 16-17, 21,39). Many studies has reported the role of phytochemicals in wound healing as tannins are seen to be active detoxifying agents and inhibit bacterial growth (42), terpenoids have astringent and antimicrobial property which promote the wound healing process (43), flavonoids and polyphenols shows the antioxidants activity by scavenging the free radical and possess anti-inflammatory properties and have antimicrobial activities (44)(45)(46)(47)(48)(49). Therefore, phytochemicals present in the crude extract may contribute to wound healing activities independently or synergistic effects.…”

Protective Effect of Wheat and Barley Grass Against the Acute Toxicological Effects of the Concurrent Administration of Excessive Heavy Metals in Drinking Water on the Rats Liver and Brain

“…The major phytocomponents reported were dodecane, 9-octadecenoic acid, and ethyliso-allocholate. Panthi et al [59] also found that the GC-MS analysis of a methanolic extract of barley grass contained fatty acids like 13-docosenic acid and 9,12,15octadecatrienoic acid. They have cancer-preventive, nematicide, anti-arthritic, antiandrogenic, anti-inflammatory, and hypocholesterolemic properties [60].…”