Bioanalytical advances in assays for C-reactive protein

Vashist, Sandeep Kumar; Venkatesh, A. G.; Schneider, E. Marion; Beaudoin, Christopher; Luppa, Peter B.; Luong, John H. T.

doi:10.1016/j.biotechadv.2015.12.010

Cited by 125 publications

(105 citation statements)

References 119 publications

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“…The developed immunoassay is superior to most of the current analysis techniques for detection of CRP in terms of highly sensitivity [40, 41]. The common clinical laboratory assays used to quantify CRP have a lower detection range in μg/mL, including latex agglutination or commercially available POCT methods.…”

Section: Resultsmentioning

confidence: 99%

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Feng³

et al. 2017

J Nanobiotechnol

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BackgroundThe conventional and widely used enzyme-linked immunosorbent assays (ELISA), due to specificity and high-sensitivity, were suitable in vitro diagnosis. But enzymes are vulnerable to the external conditions, and the complex operation steps limit its application. Semiconductor quantum dots have been successfully used in biological and medical research due to the high photoluminescence and high resistance to photobleaching. In this study, we have developed a novel quantum dot-labeled immunosorbent assay for rapid disease detection of C-reactive protein (CRP).ResultsThe assay for the detection of CRP can provide a wide analytical range of 1.56–400 ng/mL with the limit of detection (LOD) = 0.46 ng/mL and the limit of quantification = 1.53 ng/mL. The precision of the assay has been confirmed for low coefficient of variation, less than 10% (intra-assay) and less than 15% (inter-assay). The accuracy of assay meets the requirements with the recoveries of 95.4–105.7%. Furthermore, clinical samples have been collected and used for correlation analysis between this FLISA and gold standard Roche immunoturbidimetry. It shows excellent accurate concordance and the correlation coefficient value (R) is as high as 0.989 (n = 34).ConclusionsThis in vitro quantum dot-based detection method offers a lower LOD and a wide liner detection range than ELISA. The total reaction time is only 50 min, which is much shorter than the commercialization ELISA (about 120 min). All of the results show that a convenient, sensitive, and accurate fluorescence-linked immunosorbent assay method has been well established for the detection of CRP samples. Therefore, this method has immense potential for the development of rapid and cost-effective in vitro diagnostic kits.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-017-0267-4) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Feng³

et al. 2017

J Nanobiotechnol

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“…In pediatrics, in cases of neonatal sepsis, a concentration above 5 µg/ml is an indicator of a critical state. At first, a highsensitivity assay (detection of C-reactive protein in the range of 0.08-80 µg/ml) is carried out, and then the regular one, which allows for the detection of the marker in the range of 0.2-480 µg/ml (if the marker concentration is higher than 80 µg/ml) [59]. The authors of other studies chose 1 µg/ml as the cut-off value [60].…”

Section: C-reactive Protein As a Marker Of Acute Phase Of Inflammationmentioning

confidence: 99%

Monitoring Antibiotics and Inflammatory Markers in Human Blood: Impact in Choice of Antibiotic Therapy and Used Methods

Berlina¹,

Zherdev²,

Dzantiev³

2019

Preprint

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In the modern world, the problem of antibiotic therapy is acute. Despite the diversity of existing antibiotic drugs, their efficacy decreases as new, resistant forms of pathogenic microorganisms emerge. It is extremely difficult to control such processes and even more difficult to treat severe bacterial infections. In such situations, an individual approach to each patient is required and physicians need parameters to estimate the efficacy of antibiotic therapy. This review discusses the significance of monitoring the content of antibiotics in the blood for this purpose, in combination with the content of inflammatory markers, such as C-reactive protein and procalcitonin. The basic principles of antibiotic therapy, and factors in the resistance of microorganisms to antibiotics, are examined. Approaches to assess the efficacy of antibiotic therapy, as well as methods to detect antibiotics and inflammatory markers in the blood of patients, and comparative assessment of their capabilities and limitations, are described.

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“…In the context of pathogen‐independent inflammation, CRP binds DNA and histones and scavenges the nuclear materials released from damaged circulating cells to activate innate immune cells . High CRP levels are expressed in acute infection and/or inflammation and are measured in serum by a conventional CRP assay, while lower CRP levels implicated in increased CV risk are detected by a high‐sensitivity assay .…”

Section: Inflammation Biomarkersmentioning

confidence: 99%

“…High CRP levels are expressed in acute infection and/or inflammation and are measured in serum by a conventional CRP assay, while lower CRP levels implicated in increased CV risk are detected by a high‐sensitivity assay . Among several assays developed, the ultrasensitive solid‐phase ELISA, the immunoturbidimetric assay and the immunophelometry (laser nephelometry) assay have proved to be the most reliable and a good analytical agreement between all three methods has been demonstrated . Indeed, they are used indifferently .…”

Section: Inflammation Biomarkersmentioning

confidence: 99%

Cardiovascular biomarkers in clinical studies of type 2 diabetes

Baldassarre

Andersen

Consoli

et al. 2018

Diabetes Obesity Metabolism

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When planning cardiovascular (CV) studies in type 2 diabetes (T2D), selection of CV biomarkers is a complex issue. Because the pathophysiology of CV disease (CVD) in T2D is multifactorial, ideally, the selected CV biomarkers should cover all aspects of the known pathophysiology of the disease. This will allow the researcher to distinguish between effects on different aspects of the pathophysiology. To this end, we discuss a host of biomarkers grouped according to their role in the pathogenesis of CVD, namely: (1) cardiac damage biomarkers; (2) inflammatory biomarkers; and (3) novel biomarkers (oxidative stress and endothelial dysfunction biomarkers). Within each category we present the best currently validated biomarkers, with special focus on the population of interest (people with T2D). For each individual biomarker, we discuss the physiological role, validation in the general population and in people with T2D, analytical methodology, modifying factors, effects of glucose-lowering drugs, and interpretation. This approach will provide clinical researchers with the information necessary for planning, conducting and interpreting results from clinical trials. Furthermore, a systematic approach to selection of CV biomarkers in T2D research will improve the quality of future research.

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Bioanalytical advances in assays for C-reactive protein

Cited by 125 publications

References 119 publications

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Monitoring Antibiotics and Inflammatory Markers in Human Blood: Impact in Choice of Antibiotic Therapy and Used Methods

Cardiovascular biomarkers in clinical studies of type 2 diabetes

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