Bioassay guided fractionation and identification of active anti-inflammatory constituent from<i>Delonix elata</i>flowers using RAW 264.7 cells

Saravanan, S.; Ibrahim, Hairul-Islam Mohamed; David, Harding; Sundaram, R. Lakshmi; Chellappandian, M.; Balakrishna, K.; Rajendran, R.; Vijayaraghavan, Pooja; Paulraj, Michael Gabriel; Ignacimuthu, S.

doi:10.3109/13880209.2014.913067

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Different parts of Delonix elata proved to posses anti-inflammatory and anti-arthritic activities experimentally (Saravanan and Hairul, 2015, Murugananthan and Mohan, 2011, Manimekalai et al, 2011). These effects may explain its use for ear problems.…”

Section: Resultsmentioning

confidence: 99%

A survey on traditional plants used in Al Khobah village

Abdel‐Kader

Hazazi

Elmakki

et al. 2018

Saudi Pharmaceutical Journal

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The use of traditional medicine for treatment of various diseases is a common practise in most of the developing countries including the Kingdom of Saudi Arabia especially in rural areas. In this survey, Al Khobah village was selected to study the status of Traditional Medicine. Al Khobah village is located on the Southern boarders of the kingdom and characterized by diverse topography and moderate weather. The plants with medicinal uses were collected, identified and their methods of preparation and uses were recorded. After gathering all these information from the local people, literature survey was conducted on each plant to get information about pharmacological activities and weather they support the traditional use or not. The survey revealed that the uses of some plants are consistent with the experimental data in the literature. Some other plants were studied; however, they still need pharmacological investigation to prove the claimed uses. Other plant did not subject to any scientific investigation.

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Section: Resultsmentioning

confidence: 99%

A survey on traditional plants used in Al Khobah village

Abdel‐Kader

Hazazi

Elmakki

et al. 2018

Saudi Pharmaceutical Journal

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“…In inflammatory diseases, CD4 T cells serve as a potential source of proinflammatory cytokines. Depending on the pattern of regulatory transcriptional factors such as SOX5, AHR and NFkB, the polarization of autoimmune diseases progresses in host cell differentiation and maturation [ 36 , 37 ]. The MOG-antigenic stimulation in splenocytes was attenuated by BEY treatment, as evidenced by the substantial suppression of the SOX-5 expression level.…”

Section: Discussionmentioning

confidence: 99%

Probiotic-Fermented Camel Milk Attenuates Neurodegenerative Symptoms via SOX5/miR-218 Axis Orchestration in Mouse Models

et al. 2023

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Multiple sclerosis is an autoimmune-mediated myelin damage disorder in the central nervous system that is widespread among neurological patients. It has been demonstrated that several genetic and epigenetic factors control autoimmune encephalomyelitis (EAE), a murine model of MS, through CD4+ T-cell population quantity. Alterations in the gut microbiota influence neuroprotectiveness via unexplored mechanisms. In this study, the ameliorative effect of Bacillus amyloliquefaciens fermented in camel milk (BEY) on an autoimmune-mediated neurodegenerative model using myelin oligodendrocyte glycoprotein/complete fraud adjuvant/pertussis toxin (MCP)-immunized C57BL6j mice is investigated. Anti-inflammatory activity was confirmed in the in vitro cell model, and inflammatory cytokines interleukins IL17 (from EAE 311 to BEY 227 pg/mL), IL6 (from EAE 103 to BEY 65 pg/mL), IFNγ (from EAE 423 to BEY 243 pg/mL) and TGFβ (from EAE 74 to BEY 133 pg/mL) were significantly reduced in BEY-treated mice. The epigenetic factor miR-218-5P was identified and confirmed its mRNA target SOX-5 using in silico tools and expression techniques, suggesting SOX5/miR-218-5p could serve as an exclusive diagnostic marker for MS. Furthermore, BEY improved the short-chain fatty acids, in particular butyrate (from 0.57 to 0.85 µM) and caproic (from 0.64 to 1.33 µM) acids, in the MCP mouse group. BEY treatment significantly regulated the expression of inflammatory transcripts in EAE mice and upregulated neuroprotective markers such as neurexin (from 0.65- to 1.22-fold) (p < 0.05), vascular endothelial adhesion molecules (from 0.41- to 0.76-fold) and myelin-binding protein (from 0.46- to 0.89-fold) (p < 0.03). These findings suggest that BEY could be a promising clinical approach for the curative treatment of neurodegenerative diseases and could promote the use of probiotic food as medicine.

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“…To isolate bioactive molecules, bioassay-guided fractionation is commonly employed [ 12 ]. This has led to the study of a wide variety of bioactive substances present in fractions and subfractions during the isolation process [ 12 ]. Hence, this strategy was used in this work to isolate and identify antitumor metabolites from Inga species.…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Inga Species with Antitumor Activity

et al. 2022

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This work evaluated the metabolic profiling of Inga species with antitumor potential. In addition, we described the antigenotoxicity of polyphenols isolated from I. laurina and a proteomic approach using HepG2 cells after treatment with these metabolites. The in vitro cytotoxic activity against HepG2, HT-29 and T98G cancer cell lines was investigated. The assessment of genotoxic damage was carried out through the comet assay. The ethanolic extract from I. laurina seeds was subjected to bioassay-guided fractionation and the most active fractions were characterized. One bioactive fraction with high cytotoxicity against HT-29 human colon cancer cells (IC50 = 4.0 µg mL−1) was found, and it was characterized as a mixture of p-hydroxybenzoic acid and 4-vinyl-phenol. The I. edulis fruit peel (IC50 = 18.6 µg mL−1) and I. laurina seed (IC50 = 15.2 µg mL−1) extracts had cytotoxic activity against the cell line T98G, and its chemical composition showed a variety of phenolic acids. The chemical composition of this species indicated a wide variety of aromatic acids, flavonoids, tannins, and carotenoids. The high concentration (ranging from 5% to 30%) of these polyphenols in the bioactive extract may be responsible for the antitumor potential. Regarding the proteomic approach, we detected proteins directly related to the elimination of ROS, DNA repair, expression of tumor proteins, and apoptosis.

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Bioassay guided fractionation and identification of active anti-inflammatory constituent fromDelonix elataflowers using RAW 264.7 cells

Cited by 4 publications

References 25 publications

A survey on traditional plants used in Al Khobah village

A survey on traditional plants used in Al Khobah village

Probiotic-Fermented Camel Milk Attenuates Neurodegenerative Symptoms via SOX5/miR-218 Axis Orchestration in Mouse Models

Metabolic Profiling of Inga Species with Antitumor Activity

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