Bioassay technique using nonspecific esterase activities of tetrahymena pyriformis for screening and assessing cytotoxicity of xenobiotics

Abstract

A simple and rapid test for screening and assessing the cytotoxicity of xenobiotics was developed with Tetrahymena pyriformis. The method estimates the activities of nonspecific esterases of a cell by concentrating within it a specific amount of fluorescence associated with fluorescein dye. The 1‐h median effective concentration (EC50) values of 10 inorganic and eight organic substances are presented and compared to those of three other bioassays: the conventional T. pyriformis proliferation rate 9‐h median inhibitory concentrations, the Microtox® 30‐min EC50s, and the Daphnia magna 4‐methylumbelliferyl β‐D galactoside 1‐h EC50s. A highly significant correlation was found between the results obtained with the fluorescein diacetate test and those obtained with the growth inhibition and Microtox tests. This in vivo enzymatic test showed high sensitivity to all compounds tested except Cr6+ and sodium dodecyl sulfate.