Biobarcode and sequence-responsive DNA nanocapsules for label-free and sensitive electrochemical detection of circulating tumor cells

Gan, Xiaoyan; Qiu, Feng; Yuan, Ruo; Xiang, Yun

doi:10.1016/j.snb.2022.132207

Cited by 10 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The range of detection was from 5 to 3×10 4 cells/mL with a LOD of 1 cell/mL. In addition, the monitoring of human metastatic mammary carcinoma cells (MCF‐7 cell) was performed using barcode DNA and a trigger‐responsive DNA nanocapsule [54]. The response curve of MCF‐7 cells showed a linear relationship between 50 and 1×10 5 cells/mL, and the detection limit was estimated at 8 cells/mL.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical sensing of target cells based on a peptide/single‐strand DNA probe

2023

View full text Add to dashboard Cite

To electrochemically measure human myeloid leukemia cells (K562 cells), we constructed a probe consisting of peptide/single‐strand (ss) DNA. Ac‐H6Y4C with an acetylated N‐terminal of peptide was used to enhance the probe to allow electrode responses that could detect target cells. A ss‐DNA was selected as the target cell recognition moiety. The probe exhibits properties that combine the functionalities of both DNA and peptides. The measurement principle is based on changes in the peak currents of the peptide moieties that are caused by interactions between the ss‐DNA and target cells. The peak currents were proportional to the concentration of K 562 cells that ranged from 10 to 2,000 cells/mL with a LOD of 3 cells/mL.

show abstract

Section: Resultsmentioning

confidence: 99%

Electrochemical sensing of target cells based on a peptide/single‐strand DNA probe

2023

View full text Add to dashboard Cite

show abstract

“…Shen et al reported a novel aptasensor with ultrasensitive properties using cerium oxide (CeO 2 ) and iridium (Ir) nanorods, where HRP [5,141] -like catalytic activity was reported for CeO 2 nanomaterials (CeO 2 NPs and CeO 2 nanorods). Additionally, iridium (Ir) did have a high potential to adsorb oxygen-containing molecules.…”

Section: Novel Materials-based Biosensors For Detection Of Cancer Cellsmentioning

confidence: 99%

Advanced electrocatalytic materials based biosensors for cancer cell detection – A review

et al. 2023

View full text Add to dashboard Cite

Herein, we have highlighted the latest developments on biosensors for cancer cell detection. Electrochemical (EC) biosensors offer several advantages such as high sensitivity, selectivity, rapid analysis, portability, low‐cost, etc. Generally, biosensors could be classified into other basic categories such as immunosensors, aptasensors, cytosensors, electrochemiluminescence (ECL), and photo‐electrochemical (PEC) sensors. The significance of the EC biosensors is that they could detect several biomolecules in human body including cholesterol, glucose, lactate, uric acid, DNA, blood ketones, hemoglobin, and others. Recently, various EC biosensors have been developed by using electrocatalytic materials such as silver sulfide (Ag2S), black phosphene (BPene), hexagonal carbon nitrogen tube (HCNT), carbon dots (CDs)/cobalt oxy‐hydroxide (CoOOH), cuprous oxide (Cu2O), polymer dots (PDs), manganese oxide (MnO2), graphene derivatives, and gold nanoparticles (Au‐NPs). In some cases, these newly developed biosensors could be able to detect cancer cells with a limit of detection (LOD) of 1 cell/mL. In addition, many remaining challenges have to be addressed and validated by testing more real samples and confirm that these EC biosensors are more accurate and reliable to measure cancer cells in the blood and salivary samples.

show abstract

“…[8] For instance, the use of anti-EpCAM antibody/aptamer as a capture probe is one of the most studied methods for selectively capturing CTCs with high expression EpCAM (e.g., human breast cancer MCF-7 cells), however, it cannot capture CTCs with low expression EpCAM due to EMT or CTCs without EpCAM expression (e.g., human cervical cancer HeLa cells), resulting in inaccurate detection. [4] In addition, the concentration of CTCs in peripheral blood is extremely low, and it may be even lower in cancer patients without significant metastasis, [23][24][25] which further restricts the clinical application of LMMs. For efficient and ultrasensitive detection of heterogeneous CTCs, it is crucial to develop a multifunction LMM system that integrates high specificity, ideal antileukocyte adsorption performance, and signal amplification strategy.…”

Section: Introductionmentioning

confidence: 99%

Engineering Biomimetic Biosensor Using Dual‐Targeting Multivalent Aptamer Regulated 3D DNA Walker Enables High‐Performance Detection of Heterogeneous Circulating Tumor Cells

Jia,

Hu,

Zhang

et al. 2023

Small

View full text Add to dashboard Cite

The phenotypic heterogeneity of circulating tumor cells (CTCs) and the nonspecific adsorption of background cells impede the effective and sensitive detection of rare CTCs. Although leukocyte membrane coating approach has a good antileukocyte adhesion ability and holds great promise for addressing the challenge of capture purity, its limited specificity and sensitivity prevent its use in the detection of heterogeneous CTCs. To overcome these obstacles, a biomimetic biosensor that integrated dual‐targeting multivalent aptamer/walker duplex functionalized biomimetic magnetic beads and an enzyme‐powered DNA walker signal amplification strategy is designed. As compared to conventional leukocyte membrane coating, the biomimetic biosensor achieves efficient and high purity enrichment of heterogeneous CTCs with different epithelial cell adhesion molecule (EpCAM) expression while minimizing the interference of leukocytes. Meanwhile, the capture of target cells can trigger the release of walker strands to activate an enzyme‐powered DNA walker, resulting in cascade signal amplification and the ultrasensitive and accurate detection of rare heterogeneous CTCs. Importantly, the captured CTCs remained viable and can be recultured in vitro with success. Overall, this work provides a new perspective for the efficient detection of heterogeneous CTCs by biomimetic membrane coating and paves the way for early cancer diagnosis.

show abstract

Biobarcode and sequence-responsive DNA nanocapsules for label-free and sensitive electrochemical detection of circulating tumor cells

Cited by 10 publications

References 33 publications

Electrochemical sensing of target cells based on a peptide/single‐strand DNA probe

Electrochemical sensing of target cells based on a peptide/single‐strand DNA probe

Advanced electrocatalytic materials based biosensors for cancer cell detection – A review

Engineering Biomimetic Biosensor Using Dual‐Targeting Multivalent Aptamer Regulated 3D DNA Walker Enables High‐Performance Detection of Heterogeneous Circulating Tumor Cells

Contact Info

Product

Resources

About