Biocatalysis with hydroperoxide lyase in extracts from<i>Penicillium camemberti</i>in neat organic solvent media

Kuldamrong, Watchareeya; Husson, Florence; Kermasha, Sélim

doi:10.3109/10242422.2013.776545

Cited by 6 publications

(1 citation statement)

References 26 publications

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“…Oxidoreductases, including alcohol dehydrogenases and peroxidases as well as alcohol and phenol oxidases were found to be active in neat organic solvents in the 1980s, and further studies on oxidoreductases were conducted during this decade . Examples with lyases comprise hydroxynitrile lyases, cyclases, decarboxylases, hydroperoxide lyases and benzaldehyde lyases …”

Section: Introductionmentioning

confidence: 99%

Continuous Flow Bioamination of Ketones in Organic Solvents at Controlled Water Activity using Immobilized ω‐Transaminases

et al. 2020

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Compared with biocatalysis in aqueous media, the use of enzymes in neat organic solvents enables increased solubility of hydrophobic substrates and can lead to more favorable thermodynamic equilibria, avoidance of possible hydrolytic side reactions and easier product recovery. ω-Transaminases from Arthrobacter sp. (AsRÀ ωTA) and Chromobacterium violaceum (CvÀ ωTA) were immobilized on controlled porosity glass metal-ion affinity beads (EziG) and applied in neat organic solvents for the amination of 1phenoxypropan-2-one with 2-propylamine. The reaction system was investigated in terms of type of carrier material, organic solvents and reaction temperature. Optimal conditions were found with more hydrophobic carrier materials and toluene as reaction solvent. The system's water activity (a w ) was controlled via salt hydrate pairs during both the biocatalyst immobilization step and the progress of the reaction in different nonpolar solvents. Notably, the two immobilized ωTAs displayed different optimal values of a w , namely 0.7 for EziG 3 À AsRÀ ωTA and 0.2 for EziG 3 À CvÀ ωTA. In general, high catalytic activity was observed in various organic solvents even when a high substrate concentration (450-550 mM) and only one equivalent of 2propylamine were applied. Under batch conditions, a chemical turnover (TTN) above 13000 was obtained over four subsequent reaction cycles with the same batch of EziG-immobilized ωTA. Finally, the applicability of the immobilized biocatalyst in neat organic solvents was further demonstrated in a continuous flow packedbed reactor. The flow reactor showed excellent performance without observable loss of enzymatic catalytic activity over several days of operation. In general, ca. 70% conversion was obtained in 72 hours using a 1.82 mL flow reactor and toluene as flow solvent, thus affording a space-time yield of 1.99 g L À 1 h À 1 . Conversion reached above 90% when the reaction was run up to 120 hours.

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