Biocatalyst Identification and Scale‐Up: Molecular Biology for Chemists

McClean, Kathleen

doi:10.1002/9780470748589.ch2

Cited by 1 publication

(1 citation statement)

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“…The latter topic has been greatly propelled by advances in the field of molecular biology and genetic engineering, which has led to the development of new recombinant technologies that makes it possible to incorporate tailor-made DNA fragments into organisms, such as Escherichia coli and Pichia pastoris, and use them as hosts for the expression of mutant enzymes with novel properties. Unfortunately, it is beyond the scope of this Perspective to summarize all key contributions to this vast research area, and therefore we kindly refer interested readers to a number of excellent recent reviews that cover this topic thoroughly. − Instead, our aim is to describe some selected techniques that we believe have the potential of making an impact on the field of alcohol and amine DKR.…”

Section: Methods For Improving Enzymes As Biocatalystsmentioning

confidence: 99%

Chemoenzymatic Dynamic Kinetic Resolution: A Powerful Tool for the Preparation of Enantiomerically Pure Alcohols and Amines

2015

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Chemoenzymatic dynamic kinetic resolution (DKR) constitutes a convenient and efficient method to access enantiomerically pure alcohol and amine derivatives. This Perspective highlights the work carried out within this field during the past two decades and pinpoints important avenues for future research. First, the Perspective will summarize the more developed area of alcohol DKR, by delineating the way from the earliest proof-of-concept protocols to the current state-of-the-art systems that allows for the highly efficient and selective preparation of a wide range of enantiomerically pure alcohol derivatives. Thereafter, the Perspective will focus on the more challenging DKR of amines, by presenting the currently available homogeneous and heterogeneous methods and their respective limitations. In these two parts, significant attention will be dedicated to the design of efficient racemization methods as an important means of developing milder DKR protocols. In the final part of the Perspective, a brief overview of the research that has been devoted toward improving enzymes as biocatalysts is presented.

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