Biochemical and Antioxidant Changes in Plasma, Serum, and Erythrocytes of Horses before and after a Jumping Competition

Soares, Júlio César Mendes; Zanella, Ricardo; Bondan, Carlos; Alves, Leonardo Porto; Lima, Marina Ragagnin de; Motta, Adriana Costa da; Zanella, Eraldo Lourenso

doi:10.1016/j.jevs.2011.03.017

Cited by 23 publications

(30 citation statements)

References 32 publications

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“…The highest response was in younger horses which were transported over a short distance and rested for 6 h. In groups of middle-aged horses the changes were less clear. Our results are in accordance with the report of Soares et al (2011), where CK after a muscle injury was rapidly released into circulation and its activity peaked 4-12 hours later. Moreover, the 8-hour road transport resulted in an increase of both enzymes CK and AST in cold-blooded horses (Niedźwiedź et al 2012).…”

Section: Transport Induced Inflammatory Responses In Horsessupporting

confidence: 93%

“…The activities of CK and AST in plasma are commonly used as indicators of skeletal muscle damage in horses (Linder and Hatzipanagiotou 1998). Thus, some studies described changes in these enzymes after exercise (Soares et al 2011), when elevated AST and CK activities demonstrated muscle cell damage and leakage of these enzymes into the circulation. Fewer reports concern changes in enzymes caused by transportation of horses.…”

Section: Transport Induced Inflammatory Responses In Horsesmentioning

confidence: 99%

“…As estimated previously, in horses oxidative stress may play a role in pathogenesis of recurrent airway obstruction, joint disease, grass sickness, Cushing disease and urticaria (Omnaz et al 2011). Under normal conditions natural antioxidant defense is sufficient to neutralize reactive oxygen species (ROS) (Soares et al 2011). However, excessive production of ROS may cause oxidative stress, lipid peroxidation and oxidative damage of tissues, including muscles.…”

mentioning

confidence: 99%

“…The muscle enzyme activity is normally low in plasma, since they are within myocyte, however, after exercise or muscle injury their activity significantly rises due to increased cellular permeability, cell necrosis, deficient elimination or increased synthesis (Soares et al 2011). Although some studies described the activity of these enzymes after exercises, there are few reports about their activity after transportation.…”

mentioning

confidence: 99%

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Transport induced inflammatory responses in horses

Wessely-Szponder¹,

Bełkot²,

Bobowiec³

et al. 2015

Polish Journal of Veterinary Sciences

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Deleterious response to road transport is an important problem in equine practice. It determines different physiological, immunological and metabolic changes which lead to increased susceptibility to several disorders such as pneumonia, diarrhea, colics, laminitis, injuries and rhabdomyolisis. The aim of our study was to look for possible relationships between transportation of female young and older horses over a long and short distance and an inflammatory state reflected by an increase of acute phase protein concentration, oxidative stress and muscle injury. The study was conducted on 24 cold-blooded female horses divided into four groups. Six fillies aged 6-18 months and six mares aged 10-12 years were transported over the distance of about 550 km, six fillies aged 6-18 months and six mares aged 10-12 years were transported over the distance of about 50 km. Plasma and serum were obtained from blood samples taken before transportation (T0), immediately after transportation (T1) and at an abattoir during slaughter (T2). In these samples fibrinogen, MDA, AST and CK were assessed. Fibrinogen increased in all studied groups especially in fillies after long distance transportation, where it reached 205±7.07 mg/dl before transportation, 625±35.35 mg/dl after transportation, and 790±14.14 mg/dl during slaughter. MDA concentrations rose after transportation and reached the maximal level during slaughter. CK activity was more elevated after short transportation in younger horses, whereas initial activity of AST was higher in older horses. We estimated that intensified responses from acute phase, oxidative stress and muscle injury parameters indicated an inflammatory state.

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Section: Transport Induced Inflammatory Responses In Horsessupporting

confidence: 93%

Section: Transport Induced Inflammatory Responses In Horsesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Transport induced inflammatory responses in horses

Wessely-Szponder¹,

Bełkot²,

Bobowiec³

et al. 2015

Polish Journal of Veterinary Sciences

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“…Physiologic antioxidant defenses neutralize ROS activity but, the high production of ROS can result in increased rate of cellular damage (Zanella et al, 2010;Soares et al, 2011). ROS can induce sperm cells damage, causing oxidative damage to the cell membrane, starting lipid peroxidation cascade, through of attack of polyunsaturated fatty acids (Ogbuewu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Oxidative Status of Boar Semen during Storage

Zanella¹,

Zanella²,

Poetini³

et al. 2016

American Journal of Biochemistry and Biotechnology

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Abstract:The intensification of the use of artificial insemination in the swine industry has increased the interest to develop proper conditions to store semen better for longer periods of time without affecting its fertility. Trolox is a vitamin E analog with antioxidant property to prevent oxidative stress. Therefore, the objective of this study was to evaluate the Trolox antioxidant capacity on boar semen during storage period (86 h) at 15°C. For that, semen samples from 5 different boars were collected and diluted with a Long-Term (LT) extender with and without the addition of Trolox in a concentration of 50 µM. The sperm motility, oxidative stress and antioxidant protection were measured in those samples during an 84 h storage time. Our results indicated that Trolox was able to minimize the loss of sperm motility after 24 h of storage. The storage time was positively correlated with TBARS production. However in the presence of Trolox, after 12 h, TBARS content were significantly lower than those of sperm cells stored with LT diluent only. The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with LT diluent when compared to the group supplemented with Trolox. Catalase and Superoxide Dismutase activities were decreased after 48 h storage at 15°C in both groups (with or without Trolox). Spontaneous spermatozoa ROS formation was high in viable swine sperm during the LT storage period in samples without Trolox. In conclusion, LT diluent with the addition of antioxidant as Trolox can reduce the oxidative stress in sperm cells, which could be used to protect spermatozoa against oxidative damage, preventing motility losses in swine semen.

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Dataset concerning haematological and biochemical parameters changes in show jumping horses subjected to exercise and plasmapheresis session

et al. 2019

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This article presents data on the effect of plasmapheresis on clinical, haematological and biochemical parameters in horses following exercise and after a plasmapheresis session. This blood filtration technique was realised on six jumping horses (plasmapheresis group) that underwent three consecutive days of graded physical exercise. The control group (n = 6) went through the same exercise but was not subjected to the plasmapheresis session. Blood was sampled before and after each exercise, also at the beginning and the end of plasmapheresis session. The presented data was obtained by measuring clinical and haemato-biochemical parameters in both groups. The heart and respiratory rates and rectal temperature were recorded. In addition, the number of red blood and white cells, platelets also of lymphocytes, monocytes, eosinophils and granulocytes were counted. Other haematological parameters including, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration were determined. Concerning the biochemical parameters, the concentrations of albumin, globulin, total protein, glucose, alkaline phosphatase, aspartate aminotransferase, gamma glutamyl transferase, total bilirubin, lactate, creatinine kinase, urea, creatinine, calcium, sodium and potassium were measured. All parameters data were analyzed by a two-way repeated measures analysis of variance followed by Holm-Sidak post-hoc procedure to evaluate the effect of plasmapheresis and time. This paper contains data related to and supporting research articles currently published entitled “Plasmapheresis effect on haematological and biochemical parameters in athletic horses subjected to exercise” (Daden et al., 2019) [1].

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Biochemical and Antioxidant Changes in Plasma, Serum, and Erythrocytes of Horses before and after a Jumping Competition

Cited by 23 publications

References 32 publications

Transport induced inflammatory responses in horses

Transport induced inflammatory responses in horses

Oxidative Status of Boar Semen during Storage

Dataset concerning haematological and biochemical parameters changes in show jumping horses subjected to exercise and plasmapheresis session

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