Biochemical and Genetic Polymorphisms for Carboxylesterase and Acetylesterase in Grape Clones of Vitis vinifera L. (Vitaceae) Cultivars

Orasmo, Gleice Ribeiro; Oliveira-Collet, Sandra Aparecida de; Lapenta, Ana Sílvia; Machado, Maria de Fátima Pires da Silva

doi:10.1007/s10528-007-9103-0

Cited by 16 publications

(19 citation statements)

References 15 publications

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“…The level of polymorphism found in our study (65.1%) corroborated the high frequency of the null Est-3 carboxylesterase phenotypes (61.7%) in the four V. vinifera clones (Orasmo et al, 2007) and also previous RAPD studies using different cultivars or varieties of grapes (Fanizza et al, 1999;Regner et al, 2000;Tamhankar et al, 2001;Luo and He, 2001). DNA polymorphism in clones of the other 4 V. vinifera varieties has been detected using Southern hybridization (restriction fragment length polymorphism) and amplification (RAPD and inter-simple sequence repeat (SSR) sequences) techniques (Barysheva et al, 2003).…”

Section: Discussionsupporting

confidence: 90%

“…However, phenotypic reversion occurring either on a side branch or on the same bunch of the cluster of Benitaka cultivar (0.4%) and Rubi cultivar (0.27%), which exhibited the Italia cultivars' green color of the berry skin, and Brasil cultivar (28.57%), which exhibited the Benitaka cultivars' rosy-red color of the berry skin reported by Oliveira-Collet et al (2005), indicates genetic instability and a berry skin color polymorphism in grape cultivars of V. vinifera growing in the Marialva region. High frequency of the null Est-3 carboxylesterase phenotype (61.7%) also suggests the occurrence of genetic variations within cultivars (Orasmo et al, 2007).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Genetic diversity in somatic mutants of grape (Vitis vinifera) cultivar Italia based on random amplified polymorphic DNA

Maia

Mangolin²,

Collet³

et al. 2009

Genet. Mol. Res.

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AbStrACt. Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism and to examine relationships among four table grape clones from northwestern Paraná, in southern Brazil. The 10 primers used for RAPD fingerprints generated 126 reproducible fragments, of which 63, 68, 76, and 72 were polymorphic in cultivars Italia, Rubi, Benitaka, and Brasil, respectively. Among the primers, OPP-08 generated the highest number of fragments, whereas OPE-15 was the most efficient for discriminating polymorphic fragments. The distribution of the clones by cluster analysis indicated that there were no differences in RAPD markers between the colored mutant and the original clone (cultivar Italia), supporting the hypothesis that the non-colored and the colored mutant are the same cultivar. However, we found high levels of polymorphism within and between the cultivars Italia, Rubi, Benitaka, and Brasil (65.1%), contrary to a previous hypothesis that the four clones are genetically uniform. This confirmed our expectation of genetic variation among the clones and within each clone. We conclude that the primers are useful for analyzing the development of the genetic diversity within each of these clones.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 98%

Genetic diversity in somatic mutants of grape (Vitis vinifera) cultivar Italia based on random amplified polymorphic DNA

Maia

Mangolin²,

Collet³

et al. 2009

Genet. Mol. Res.

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“…Most esterase isozymes in plant tissues have been classified as carboxylesterases (Pereira et al, 2001;Carvalho et al, 2003;Orasmo et al, 2007). The carboxylesterase activity in plants has been correlated with differentiation processes (Gahan et al, 1983;Melati et al, 1996), and different physiological roles have recently been attributed to these compounds.…”

Section: Discussionmentioning

confidence: 99%

“…Polyacrylamide gel electrophoresis (PAGE) has been used to analyze α-and β-esterase genetic polymorphism in plants (Pereira et al, 2001;Carvalho et al, 2003;Orasmo et al, 2007;Frigo et al, 2009) and is also useful for analyses of the genetic diversity and structure of Capsicum accessions maintained at BAGC. Esterases are often found in multigene families (Robin et al, 1996), and a large number of esterase loci in plant tissues can be used simultaneously for the identification of genetic variation and polymorphism analyses.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of Capsicum spp germplasm bank accessions based on α/β-esterase polymorphism

Monteiro

Bronzato²,

Orasmo³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Genetic diversity and structure were analyzed in 10 accessions belonging to Banco Ativo de Germoplasma de Capsicum located at Federal University of Piauí in northwestern Brazil that receives pepper samples grown in community gardens in various regions and Brazilian states. Selections were made from seeds of C. chinense (4 accessions), C. annuum (5 accessions), and C. baccatum (1 accession). Samples consisting of leaves were collected from 4-10 plants of each accession (a total of 85 plants). Native polyacrylamide gel electrophoresis was used to identify α-and β-esterase polymorphisms. Polymorphism was clearly detected in 5 loci. Sixteen alleles were found at 5 α/β-esterase loci of the three Capsicum species. In the C. chinense samples, the highest H O and H E values were 0.3625 and 0.4395, respectively, whereas in C. annuum samples, H O and H E values were 0.2980 and 0.3310, respectively; the estimated H O and H E values in C. chinense samples were higher than those detected in C. annuum samples. A deficit of homozygous individuals was found in C. chinense (F IS = -0.6978) and C. annuum (F IS = 0.7750). Genetic differentiation between C. chinense and C. annuum at these loci was high (F ST = 0.1867) indicating that C. chinense and C. annuum are genetically structured species for α/β-esterase isozymes. The esterase analysis showed high genetic diversity among the C. chinense and C. annuum samples and very high genetic differentiation (F ST = 0.6321) among the C. chinense and C. annuum samples and the C. baccatum accession.

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“…De acordo com Collet et al (2005) e Orasmo et al (2007), as alterações morfológicas que distinguem a uva 'Itália' e seus mutantes (Rubi, Benitaka e Brasil) são decorrentes de alterações em, pelo menos, sete genes regulatórios envolvidos na expressão de antocianinas, determinantes da coloração dos frutos nessas variedades. Essas descobertas indicam a instabilidade genética e o polimorfismo da cor da casca das bagas dessas cultivares de V. vinifera (MAIA et al, 2009).…”

Section: 'Black Star': a Natural Somatic Mutation From Table Grape CVunclassified

'Black star': uma mutação somática natural da uva fina de mesa cv. Brasil

Roberto

Assis

Genta³

et al. 2012

Rev. Bras. Frutic.

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RESUMO -A uva fina de mesa 'Black Star', originada de uma mutação somática da uva cv. Brasil em Marialva-PR, é descrita quanto às suas principais características físico-químicas e produtivas. Suas bagas, com sementes, apresentam formato elipsoide alongado com coloração vermelho-escura, tendendo ao preto durante a maturação plena. O ciclo, o desempenho produtivo e a suscetibilidade às doenças fúngicas assemelham-se aos da cv. Itália. Durante a maturação plena, apresenta teor médio de sólidos solúveis de 14ºBrix, 0,6% de ácido tartárico e índice de maturação de 21. Trata-se de nova cultivar de uva fina de mesa com potencial de cultivo no Brasil. Termos para indexação: Vitis vinifera L., cultivar, viticultura. 'BLACK STAR': A NATURAL SOMATIC MUTATION FROM TABLE GRAPE cv. BRAZILABSTRACT -The fine table grape 'Black Star', originated from a somatic mutation of the grape cv. Brasil in Marialva, PR, is described here as their main physico-chemical and productive characteristics. Its berries have elongated ellipsoid shape with dark red color tending to black during the full maturation. The cycle, the production performance and the susceptibility to fungal diseases is similar to the cv. Italia. During the full maturation, it has an average content of soluble solids of 14ºBrix, 0.6% of tartaric acid and maturation index of 21. This is a new cultivar of fine table grape with cultivation potential in Brazil.

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Biochemical and Genetic Polymorphisms for Carboxylesterase and Acetylesterase in Grape Clones of Vitis vinifera L. (Vitaceae) Cultivars

Cited by 16 publications

References 15 publications

Genetic diversity in somatic mutants of grape (Vitis vinifera) cultivar Italia based on random amplified polymorphic DNA

Genetic diversity in somatic mutants of grape (Vitis vinifera) cultivar Italia based on random amplified polymorphic DNA

Genetic diversity analysis of Capsicum spp germplasm bank accessions based on α/β-esterase polymorphism

'Black star': uma mutação somática natural da uva fina de mesa cv. Brasil

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