Biochemical and Hematological Study with the Appreciation of some Immunological Parameters in Thalassemia Patients at Kerbala Province

Abstract

Thalassemia is a genetic disorder occurs as a result of the imbalance in the construction of haemoglobin chains cause haemolytic anaemia. This study was aimed to evaluate the serum level of immunological parameters (Transforming growth factor beta1, Interleukin-23) and hematological parameters in patients with alpha and beta thalassemia at Kerbala province. And was conducted at Kerbala Children's Hospital / Thalassemia Department / Kerbala Governorate during the period from November 2017 to May 2018. Seventy patients involved 48 beta thalassemia patients and 22 alpha thalassemia patients with age ranged 10-20 years old and ten matched healthy controls were enrolled in the study. Result exposed non-significant increase (p>0.05) in IL-23 and TGF-β in thalassemia patients compared with control group. In β-thalassemia patients the results of the current study indicated that non-significant positive correlation between IL-23 with WBC, there was non-significant positive correlation between TGF-β1 with WBC and PLT. As for β-thalassemia patients the present study showed non-significant positive correlation between IL-23 with Hb and WBC, and there was non-significant positive correlation between TGF-β1 with WBC. The study showed changes in the immunological parameters in patients with thalassemia of both types, as the serum level of both IL-23 and TGF-β1 were raised in addition to changes in hematological and Biochemical parameters.

Could the Crosstalk Between Mdscs and Tregs Have a Role in β-thalassemia?

“…The chronic in ammation may have triggered bone marrow to generate MDSCs causing MDSC expansion and accumulation in the circulation of BTM patients. In agreement with other studies 19,20 , our BTM patients had a high level of in ammatory cells in the form of high TLC. Additionally, the direct relations observed between TLC and each of total MDSCs and MO-MDSCs may support the hypothesis mentioned above that chronic in ammation triggers bone marrow to generate these cells.…”

Could the Crosstalk Between Myeloid-Derived-Suppressor Cells and Regulatory T Cells Have a Role in Beta-Thalassemia?

“…Chronic inflammation may trigger the generation of MDSCs in the BM, causing MDSC expansion and accumulation in the circulation of TDT patients. In agreement with other studies, our TDT patients had high levels of inflammatory cells, indicated by a high TLC [ 22 , 23 ]. Additionally, the direct relationship observed between TLC and both total MDSCs and MO-MDSCs may support the hypothesis that chronic inflammation triggers the BM to generate these cells.…”