Biochemical and histological changes in the heart of post-partum rats exposed to Natron

Saidu, Yauba; Usman, Maimuna Jumai; Isa, Suleiman Ahmed; Isezuo, SA; Bilbis, L. S.; Sahabi, Saddiku Malami; Bello, A; Muhammad, S.

doi:10.1016/j.ihj.2017.12.002

Cited by 5 publications

(9 citation statements)

References 31 publications

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“…A report by Leopold and Loscalzo, (2008) stated that lipid alterations have been considered as contributory factors to OS in obesity. Saidu et al (2018) reported elevated levels of markers of cardiac function and significant changes in the heart architecture in postpartum rats following natron administration. Our data suggest that exposure of postpartum rats to natron at higher doses caused OS and dyslipidemia.…”

Section: Resultsmentioning

confidence: 99%

“…The treatment lasted for 4 weeks. These concentrations were adopted from our previous study (Saidu et al., 2018). At the end of 28 days’ period, the animals were anaesthetized using cotton wool soaked in chloroform vapor in a transparent gas jar and blood was collected from the jugular vein for analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The resulting volume overload from this practice is believed to worsen heart failure in a heart that is already under stress by hemodynamic changes in pregnancy (Davidson et al., 1974). This practice has also been linked to increased morbidity and mortality in postpartum patients in this region due to its toxicity and limited literature explaining its toxic effects (Saidu et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

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Effect of natron administration on the antioxidant status and lipid profile of rats

Saidu

Olaosebikan

Isa

et al. 2020

Journal of Food Science

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Natron consumption has been implicated in the pathogenesis of peripartum cardiomyopathy. This work evaluates the effect of natron on the antioxidant status and lipid profile of postpartum rats administered graded doses of natron for four consecutive weeks. After treatment, the rats were assessed for antioxidant status, malondialdehyde level, and lipid profile. The results revealed that natron caused a significant decrease (P ˂ 0.05) in the activity of catalase in rats administered with 300 mg/kg of natron compared to control. The activities of superoxide dismutase and glutathione peroxidase decreased in a dose‐dependent manner; however, the difference was not statistically significant when compared with control. Serum levels of antioxidant minerals were also significantly decreased (P ˂ 0.05) at higher doses of natron in comparison to control. There was a significant increase (P < 0.05) in the malondialdehyde level in rats administered with 200 and 300 mg/kg of natron when compared with control. Natron at higher doses caused a significant increase (P ˂ 0.05) in the level of the lipid profile parameters except for high‐density lipoprotein‐cholesterol that decrease significantly (P ˂ 0.05). This study demonstrated that the administration of natron at high doses induced dyslipidemia and oxidative stress in postpartum rats. Practical Application This research reports the implication of a high intake of natron to health and to establish the relationship between natron intake and peripartum cardiomyopathy (PPCM) using an animal model. Natron has health benefits; however, its consumption at high doses should be discouraged as it can lead to oxidative stress (OS) and dyslipidemia. The results suggest that OS due to natron may contribute to the pathogenesis of PPCM. A high concentration of natron can be used to induce an animal model of PPCM, which would be of practical application in studying the molecular basis and possible discovery of therapeutics for the disease.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of natron administration on the antioxidant status and lipid profile of rats

Saidu

Olaosebikan

Isa

et al. 2020

Journal of Food Science

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“…Alkaline phosphatase is present in most of the tissues; however, the bones and liver have the highest amount. The estimation of CK-MB provides information on cardiac muscles [29]. These biomarkers are leaked out from their respective localization into the blood during tissue damage.…”

Section: Discussionmentioning

confidence: 99%

Allylmethylsulfide, a Sulfur Compound Derived from Garlic, Attenuates Isoproterenol-Induced Cardiac Hypertrophy in Rats

Mohammed

Paramesha

Kumar

et al. 2020

Oxidative Medicine and Cellular Longevity

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Allylmethylsulfide (AMS) is a novel sulfur metabolite found in the garlic-fed serum of humans and animals. In the present study, we have observed that AMS is safe on chronic administration and has a potential antihypertrophic effect. Chronic administration of AMS for 30 days did not cause any significant differences in the body weight, electrocardiogram, food intake, serum biochemical parameters, and histopathology of vital organs. Single-dose pharmacokinetics of AMS suggests that AMS is rapidly metabolized into Allylmethylsulfoxide (AMSO) and Allylmethylsulfone (AMSO2). To evaluate the efficacy of AMS, cardiac hypertrophy was induced by subcutaneous implantation of ALZET® osmotic minipump containing isoproterenol (~5 mg/kg/day), cotreated with AMS (25 and 50 mg/kg/day) and enalapril (10 mg/kg/day) for 2 weeks. AMS and enalapril significantly reduced cardiac hypertrophy as studied by the heart weight to body weight ratio and mRNA expression of fetal genes (ANP and β-MHC). We have observed that TBARS, a parameter of lipid peroxidation, was reduced and the antioxidant enzymes (glutathione, catalase, and superoxide dismutase) were improved in the AMS and enalapril-cotreated hypertrophic hearts. The extracellular matrix (ECM) components such as matrix metalloproteinases (MMP2 and MMP9) were significantly upregulated in the diseased hearts; however, with the AMS and enalapril, it was preserved. Similarly, caspases 3, 7, and 9 were upregulated in hypertrophic hearts, and with the AMS and enalapril treatment, they were reduced. Further to corroborate this finding with in vitro data, we have checked the nuclear expression of caspase 3/7 in the H9c2 cells treated with isoproterenol and observed that AMS cotreatment reduced it significantly. Histopathological investigation of myocardium suggests AMS and enalapril treatment reduced fibrosis in hypertrophied hearts. Based on our experimental results, we conclude that AMS, an active metabolite of garlic, could reduce isoproterenol-induced cardiac hypertrophy by reducing oxidative stress, apoptosis, and stabilizing ECM components.

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“…It was documented that loss of the cardiomyocytes by either apoptosis or necrosis is a key feature in cardiomyopathy [23] . Damage of the myofibrils could be explained according to Elwan et al, [24] to be a consequence of mitochondrial dysfunction that leads to loss of ATP production as well as imbalance of calcium uptake.…”

Section: Discussionmentioning

confidence: 99%

Possible Protective Effect of Propolis on Cardiac Muscle Injury Induced by Liver Fibrosis in Adult Male Albino Rat: Histological Study

Elbakary

Sadek

2018

Egyptian Journal of Histology

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Introduction: Most chronic liver diseases lead to cirrhotic cardiomyopathy that occurs as a late complication in 56% of the hepatic patients. Propolis is considered as a tonic that increases the body resistance to aging. It also decreases the risk of cardiovascular diseases and atherosclerosis. Aim of the work: was to study the possible protective role of propolis on the histological changes occurred in cardiac muscle secondary to liver fibrosis. Materials and Methods: 40 healthy male albino rats were randomly divided into 4groups including: control group (group I), propolis treated group (group II) given 20mg propolis once daily orally for 4 weeks, (group III) received 0.2 ml/100 gm carbon tetrachloride by subcutaneous injection for 4 weeks and (group IV) received both carbon tetrachloride and propolis at the same dose of group IIand III for the same period. At the end of the experiment, all the rats were sacrificed, and cardiac muscle was obtained and processed for light and electron microscopic examination. Results: Group III revealed widely separated cardiomyocytes with excessive connective tissue nuclei between them. Cytoplasmic vacuolations and marked fibrillolysis were observed. Cellular infiltration and dilated blood vessels were found. Excessive collagen fibers were detected by Mallory stain. Ultrastructural changes included irregular sarcomeres, interrupted intercalated disks, degenerated mitochondria and dilated sarcoplasmic reticulum. Group IV showed partial improvement with regular arrangement of cardiac fibers and sarcomeres. Conclusions: Use of propolis partially improved the histological changes that occurred in the cardiac muscle as a result of liver fibrosis.

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Biochemical and histological changes in the heart of post-partum rats exposed to Natron

Cited by 5 publications

References 31 publications

Effect of natron administration on the antioxidant status and lipid profile of rats

Effect of natron administration on the antioxidant status and lipid profile of rats

Allylmethylsulfide, a Sulfur Compound Derived from Garlic, Attenuates Isoproterenol-Induced Cardiac Hypertrophy in Rats

Possible Protective Effect of Propolis on Cardiac Muscle Injury Induced by Liver Fibrosis in Adult Male Albino Rat: Histological Study

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