Biochemical and histopathological study in rats intoxicated with carbontetrachloride and treated with camel milk

Abstract

The unique characters of camel’s milk make it used extensively in the field of medicine as anti-microbial, anti-diabetic and hepatoprotective agent. The lack of studies demonstrating the protective effect of camel’s milk against hepatotoxic compound was the main reason beyond the conduction of the current experiment which aimed to investigate the protective effects of camel’s milk against carbontetrachloride (CCl4) induced hepatotoxicity. Therefore, 24 rats were fed on standard diet and divided into four groups. Rats of the first group and second groups were injected i/p with paraffin oil and received either tap water (control 1) or camel’s milk (control 2), respectively. Rats of the third and fourth groups were injected i/p with CCl4 and received either tap water or camel’s milk, respectively. At the end of the experiment (5 weeks), blood and liver samples were collected for biochemical and histopathological analysis. The present findings revealed that, CCl4 elevated serum enzyme activities of liver and some biochemical parameters, but these effects were prevented by the treatment of rats with camel milk. Histopathologically, a great amount of mononuclear cells infiltration, necrotic cells and few fibroblasts were observed in liver of CCl4 treated group. The present study concluded that camel milk treatment may play a protective role against CCl4-induced liver damages in rats. These protective effects were in the form of improving of liver enzyme activities, blood biochemical parameters and histological picture of liver of intoxicated rats. In the future, examination of the liver protective effect of camel milk against CCl4 in dose dependant manner could be investigated.

Synergistic Effects of Jerusalem Artichoke in Combination with Pegylated Interferon Alfa-2a and Ribavirin Against Hepatic Fibrosis in Rats

“…Moreover, Masson's trichrome stain of liver sections showed congestion and thickening of the portal tract with dense fibrous tissue. Similar observations of the liver tissues in CCl 4 intoxicated rats were reported previously (Domitrovic et al, 2009;Althnaian et al, 2013).…”

“…As previously reported, administration of CCl 4 for 8 weeks resulted in a significant increase in serum ALT, AST activities as well as total billirubin levels (Mehmetcik et al, 2008;Arici and Cetin, 2011). Damaged liver architecture can lead to an increase in the serum activities of liver enzymes due to their localization in the cytoplasmic area of the cell (Althnaian et al, 2013). Theses biomarkers showed major improvement to near normal values with triplet combination, which is an indication of the stabilization of the plasma membrane and the repair of hepatic tissue damage caused by CCl 4 intoxication.…”

Protective role of Broccoli powder against continuous ingestion of Escitalopram antidepressant drug induced hepatotoxicity in Swiss albino male mice

“…(Figure 3b, 3c and 3d). Histopathological results of CCl4 were similar to Al-Ghamdi [27], Eidi et al, [28], Soujanya et al, [29], Cordeiro¹ and Kaliwal¹ [30], Althnaian et al, [31] and Abdel et al, [32].From the histopathological examination of the present work it could be concluded that broccoli powder with escitalopram drug treated group showed minimal congestion of central vein and portal vein with minimal hepatocyte necrosis near the central vein [24] …”

Functional Assessments and Histopathology of Hepatorenal Tissues of Wistar Rats Infused with Carbon Tetrachloride and Treated with Raw and Hydrothermal Processed Herbs

“…1,9,11,12,13,14 Herbal extracts have been reported to ameliorate hepatic and renal disorders in humans and animal models. 2,9,13,15 There is a vast array of Nigerian indigenous medicinal plants that are used for the treatment and management of pathologic conditions linked with overwhelming levels of reactive metabolic radicals.…”

“…5,6,7,8,9 Carbon tetrachloride (CCl 4 ) is a chemical agent noted to be toxic to hepatorenal tissues in experimental animals and human subjects. 5,6,8,10,11,12,13 Reports showed that CCl 4 toxicity is elicited by microsomal CYP2E1-induced formation of reactive trichloromethyl (CCl 3…”