Biochemical and physicochemical treatment of flax fibers

Shamolina, I. I.; Bochek, A. M.; Забивалова, Н. М.; Vlasova, E. N.; Волчек, Б. З.; Sinitsin, A. P.

doi:10.1007/s11167-005-0106-5

Cited by 9 publications

(6 citation statements)

References 2 publications

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“…Unfortunately, in recent decades the popularity of the flax plantations has decreased, not only in Poland, but all over the world [ 30 ]. This tendency could be explained by several reasons:…”

Section: Introductionmentioning

confidence: 99%

Manipulating cinnamyl alcohol dehydrogenase (CAD) expression in flax affects fibre composition and properties

et al. 2014

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BackgroundIn recent decades cultivation of flax and its application have dramatically decreased. One of the reasons for this is unpredictable quality and properties of flax fibre, because they depend on environmental factors, retting duration and growing conditions. These factors have contribution to the fibre composition, which consists of cellulose, hemicelluloses, lignin and pectin. By far, it is largely established that in flax, lignin reduces an accessibility of enzymes either to pectin, hemicelluloses or cellulose (during retting or in biofuel synthesis and paper production).Therefore, in this study we evaluated composition and properties of flax fibre from plants with silenced CAD (cinnamyl alcohol dehydrogenase) gene, which is key in the lignin biosynthesis. There is evidence that CAD is a useful tool to improve lignin digestibility and/or to lower the lignin levels in plants.ResultsTwo studied lines responded differentially to the introduced modification due to the efficiency of the CAD silencing. Phylogenetic analysis revealed that flax CAD belongs to the “bona-fide” CAD family. CAD down-regulation had an effect in the reduced lignin amount in the flax fibre cell wall and as FT-IR results suggests, disturbed lignin composition and structure. Moreover introduced modification activated a compensatory mechanism which was manifested in the accumulation of cellulose and/or pectin. These changes had putative correlation with observed improved fiber’s tensile strength. Moreover, CAD down-regulation did not disturb at all or has only slight effect on flax plants’ development in vivo, however, the resistance against flax major pathogen Fusarium oxysporum decreased slightly. The modification positively affected fibre possessing; it resulted in more uniform retting.ConclusionThe major finding of our paper is that the modification targeted directly to block lignin synthesis caused not only reduced lignin level in fibre, but also affected amount and organization of cellulose and pectin. However, to conclude that all observed changes are trustworthy and correlated exclusively to CAD repression, further analysis of the modified plants genome is necessary. Secondly, this is one of the first studies on the crop from the low-lignin plants from the field trail which demonstrates that such plants could be successfully cultivated in a field.

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Section: Introductionmentioning

confidence: 99%

Manipulating cinnamyl alcohol dehydrogenase (CAD) expression in flax affects fibre composition and properties

et al. 2014

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“…Also we suggest that the treatment of the cotton fiber and all the cellulosic fiber (cotton, linen) with α-amylase enzyme causes partial removal of concomitant substances (fats, pectins, and lignin) from cellulose. This finding is agreement with (Shamolina et al, 2004) that found the treatment On the other hand, results in Fig. 79 showed FTIR spectra of silk dyed with madder dye mordanted with alum after α-amylase treatment with different concentration for different durations (10U/ml at 1, 3 h).…”

Section: Ftir Spectra Of Fabrics Treated With α-Amylasesupporting

confidence: 91%

Development of biotechnological processes for the restoration of historical textiles

Ahmed¹

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καλά αποτελέσματα σε ήπιες και σύντομες συνθήκες κατεργασίας σε σύγκριση με τη συμβατική μέθοδο, πράγμα που θα προσέφερε σημαντική εξοικονόμηση ενέργειας, νερού και δυνατότητα εναλλακτικής χρήσης των πόρων. Δεν παρατηρήθηκαν σημαντικές αλλαγές στα βαμμένα λινά και μεταξωτά υφάσματα.

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“…samples studied (i.e., the control and transgenic fibers) and very similar to that for purified cotton cellulose, 14 but the bands differ in terms of their absorption intensity. The band could be deconvoluted into four Lorenzian components (Figure 6) at 3460, 3410, 3350, and 3300/cm.…”

Section: Vibrational Datamentioning

confidence: 81%

“…However, they are also present in the primary wall of individual fibers, and therefore are constituents of the fibers themselves. 14 We determined the content of these compounds in transgenic flax fibers and compared them with the contents for the control fibers (Figure 2). The data showed no significant changes in the contents of cellulose, lignin, or pectin.…”

Section: The Chemical Analysis Of the Stem And Fiber Constituentsmentioning

confidence: 99%

Biochemical, mechanical, and spectroscopic analyses of genetically engineered flax fibers producing bioplastic (poly‐β‐hydroxybutyrate)

Wróbel‐Kwiatkowska

Skórkowska-Telichowska²,

Dymińska

et al. 2009

Biotechnology Progress

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The interest in biofibers has grown in recent years due to their expanding range of applications in fields as diverse as biomedical science and the automotive industry. Their low production costs, biodegradability, physical properties, and perceived eco-friendliness allow for their extensive use as composite components, a role in which they could replace petroleum-based synthetic polymers. We performed biochemical, mechanical, and structural analyses of flax stems and fibers derived from field-grown transgenic flax enriched with PHB (poly-beta-hydroxybutyrate). The analyses of the plant stems revealed an increase in the cellulose content and a decrease in the lignin and pectin contents relative to the control plants. However, the contents of the fibers' major components (cellulose, lignin, pectin) remain unchanged. An FT-IR study confirmed the results of the biochemical analyses of the flax fibers. However, the arrangement of the cellulose polymer in the transgenic fibers differed from that in the control, and a significant increase in the number of hydrogen bonds was detected. The mechanical properties of the transgenic flax stems were significantly improved, reflecting the cellulose content increase. However, the mechanical properties of the fibers did not change in comparison with the control, with the exception of the fibers from transgenic line M13. The generated transgenic flax plants, which produce both components of the flax/PHB composites (i.e., fibers and thermoplastic matrix in the same plant organ) are a source of an attractive and ecologically safe material for industry and medicine.

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Biochemical and physicochemical treatment of flax fibers

Abstract: Conditions were found for separating concomitant substances from cellulose in short flax fibers by treatment with surfactant and enzyme solutions. The chemical composition of the treated fibers was analyzed, and their structural organization was studied by IR Fourier spectroscopy.

Cited by 9 publications

References 2 publications

Manipulating cinnamyl alcohol dehydrogenase (CAD) expression in flax affects fibre composition and properties

Manipulating cinnamyl alcohol dehydrogenase (CAD) expression in flax affects fibre composition and properties

Development of biotechnological processes for the restoration of historical textiles

Biochemical, mechanical, and spectroscopic analyses of genetically engineered flax fibers producing bioplastic (poly‐β‐hydroxybutyrate)

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