Biochemical and physiological changes in Dipteryx alata Vog. seeds during germination and accelerated aging

Silva, G.P.; Sales, Juliana de Fátima; Nascimento, Kelly Juliane Telles; Rodrigues, Aline Sueli de Lima; Camelo, Gessimar Nunes; Borges, Eduardo Euclydes de Lima e

doi:10.1016/j.sajb.2020.02.007

Cited by 12 publications

(5 citation statements)

References 39 publications

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“…Electrolyte leakage (total soluble sugars, soluble proteins and phosphorus), demonstrated by EC, indicates that lots with a greater level of deterioration submitted to soaking exhibit higher solute and ion concentrations due to low cell membrane integrity (Silva et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Viability and performance of wheat seedlings after artificial seed aging

2022

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Seed deterioration is a continuous irreversible process that affects cell structures and molecules and compromises the physiological quality of seeds. This study aimed to assess the effect of artificial wheat seed deterioration on germination and seedling performance. The TBIO Toruk wheat cultivar was used, with seeds submitted to different artificial aging times (2, 3, 4, 5, 6 and 7 days). The original and aged seed lots were submitted to laboratory germination and seedling performance tests (seedling length, seedling dry weight and endosperm dry weight). Alpha-amylase activity, electrical conductivity and the oxidative stress marker malondialdehyde were quantified. The exudate resulting from the electrical conductivity test was separated to quantify total soluble sugars, soluble proteins and phosphorus. The deterioration process increased lipid peroxidation and decreased initial alpha-amylase activity. During germination, the most deteriorated lots exhibited greater solute loss and lower alpha-amylase synthesis capacity. The lots with the greatest deterioration showed reduced viability and produced worse-performing seedlings.

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Section: Resultsmentioning

confidence: 99%

Viability and performance of wheat seedlings after artificial seed aging

2022

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“…To determine POD activity, 4 mL of the reaction mixture was used containing 100 mM phosphate buffer (pH 6.0), 5 mM guaiacol, 12.4 mM H 2 O 2 , and 1 mL enzyme extract. Then the absorbance of the reaction mixture was measured at 470 nm for 40 s. One POD unit was defined as 1 g fresh sample, leading to an absorbance (OD 470 nm ) value increase of 0.01 per minute [ 71 ], which was expressed in U min −1 g −1 fresh sample.…”

Section: Methodsmentioning

confidence: 99%

Effect of Isosteviol on Wheat Seed Germination and Seedling Growth under Cadmium Stress

Zhang

Gao

2021

Plants

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Cadmium (Cd) contamination of agricultural soil has become a serious threat to global food security. The present study highlights the effect of added isosteviol in modulating growth physiology and antioxidant defense systems conferring tolerance against cadmium (Cd) stress in wheat. Wheat growth, chlorophyll content, malondialdehyde (MDA) content of leaves, dehydrogenase activity of root, and antioxidant enzyme activity were determined to get an overview of cellular response in conquering Cd-induced oxidative stress damages. The results indicated that wheat germination was inhibited under Cd2+ concentration at 10 µM. The presence of isosteviol and gibberellic acid (GA) significantly alleviated the inhibitory effect on the growth of wheat seedling under 10 µM Cd2+ stress. Moreover, different concentrations of isosteviol and GA regulated the physiological changes of wheat under Cd stress: more chlorophyll a + b content; less MDA content; and higher dehydrogenase activity of root and antioxidant enzyme activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as compared to Cd alone in wheat seedling. The present study thus suggests a possible role of isosteviol in amelioration of Cd stress by increasing chlorophyll content and root dehydrogenase activity, which also could reduce oxidative damage of the cell membrane by regulating the activities of antioxidant enzymes in wheat seedling.

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“…where N is the final number of germinated seeds and n i and n j the cumulative number of seeds germinated by adjacent counts at times t i and t j when n i < (N + 1)/2 < n j . Solute leakage was determined by measuring electrical conductivity (EC) according to Silva et al (2020). Hundred mg of seeds was placed in 20 mL of deionized H 2 O for 24 h at 25 ± 3 °C.…”

Section: Seed Viability and Vigourmentioning

confidence: 99%

Redox imbalance accompanies loss of viability in seeds of two cacti species buried in situ

Guzmán-Hernández,

Barbosa-Martínez,

Villa-Hernández

et al. 2024

Seed Sci. Res.

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This work provides insights into the deterioration of cacti seeds of Escontria chiotilla (F.A.C. Weber ex K. Schum) and Stenocereus pruinosus (Otto ex Pfeiff.) Buxbaum stored ex situ at 25 °C, under dry and dark conditions or buried in situ conditions in a xerophytic shrubland. Viability, germination speed, electrolyte leakage and indicators of the redox balance including glutathione content, glutathione half-cell reduction potential (EGSSG/2GSH) and malondialdehyde (MDA), oxidized protein content, together with water-soluble antioxidant enzyme activity were assessed. Over a period of two years of storage, viability was maintained when seeds were stored ex situ at 7–9% water content compared to seeds buried in the soil. A second burial experiment showed that seeds of E. chiotilla maintained viability during a year of storage that included a rainy season followed by a dry season. Thereafter, they died rapidly during the second rainy season. In contrast, those of S. pruinosus started to lose viability after 6 months of burial at the end of the rainy season and were mostly dead at the end of the dry season. This difference in persistence between species was related to a difference in the glutathione content and antioxidant enzyme activities. In both storage experiments, the loss of viability of both species was associated to a EGSSG/2GSH shift to a more oxidative state during burial. Yet, contents in MDA and oxidized soluble proteins were not related to redox imbalance and loss of viability, indicating that these compounds are not good markers of oxidative stress in cacti seeds during storage.

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Biochemical and physiological changes in Dipteryx alata Vog. seeds during germination and accelerated aging

Cited by 12 publications

References 39 publications

Viability and performance of wheat seedlings after artificial seed aging

Viability and performance of wheat seedlings after artificial seed aging

Effect of Isosteviol on Wheat Seed Germination and Seedling Growth under Cadmium Stress

Redox imbalance accompanies loss of viability in seeds of two cacti species buried in situ

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