Biochemical and physiological characterization for nitrogen use efficiency in aromatic rice genotypes

Vijayalakshmi, P.; Vishnukiran, T.; Kumari, Bimlendra; Srikanth, B.; Rao, I; Swamy, K. N.; Surekha, K.; Sailaja, N.; Subbarao, L. V.; Rao, P. Raghuveer; Subrahmanyam, D.; Neeraja, C. N.; Voleti, S. R.

doi:10.1016/j.fcr.2015.04.012

Cited by 46 publications

(47 citation statements)

References 75 publications

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“…Thus, we assumed that in CCRI-69, the low-affinity transporters may be more active in the roots and shoots, which might lead to the high N uptake and assimilation even under low nitrogen supply, as discussed in previous studies [61,62]. Another reason for high N metabolism in CCRI-69 might be due to the constant conversion of nitrate to nitrite and then to ammonia and amino acids [63,64]. Thus, a well-coordinated system of N uptake and assimilation may be the base of the high N use efficiency in CCRI- 69 (Yun et al, 2008), which was explained by their high enzymatic activities (Figures 2 and 3).…”

Section: Variations In Morphophysiological and Biochemical Traits Amomentioning

confidence: 98%

Variations in Nitrogen Metabolism are Closely Linked with Nitrogen Uptake and Utilization Efficiency in Cotton Genotypes under Various Nitrogen Supplies

Iqbal

Dong

Wang

et al. 2020

Plants

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Cotton production is highly sensitive to nitrogen (N) fertilization, whose excessive use is responsible for human and environmental problems. Lowering N supply together with the selection of N-efficient genotypes, more able to uptake, utilize, and remobilize the available N, could be a challenge to maintain high cotton production sustainably. The current study aimed to explore the intraspecific variation among four cotton genotypes in response to various N supplies, in order to identify the most distinct N-efficient genotypes and their nitrogen use efficiency (NUE)-related traits in hydroponic culture. On the basis of shoot dry matter, CCRI-69 and XLZ-30 were identified as N-efficient and N-inefficient genotypes, respectively, and these results were confirmed by their contrasting N metabolism, uptake (NUpE), and utilization efficiency (NUtE). Overall, our results indicated the key role of shoot glutamine synthetase (GS) and root total soluble protein in NUtE. Conversely, tissue N concentration and N-metabolizing enzymes were considered as the key traits in conferring high NUpE. The remobilization of N from the shoot to roots by high shoot GS activity may be a strategy to enhance root total soluble protein, which improves root growth for N uptake and NUE. In future, multi-omics studies will be employed to focus on the key genes and pathways involved in N metabolism and their role in improving NUE.

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Section: Variations In Morphophysiological and Biochemical Traits Amomentioning

confidence: 98%

Variations in Nitrogen Metabolism are Closely Linked with Nitrogen Uptake and Utilization Efficiency in Cotton Genotypes under Various Nitrogen Supplies

Iqbal

Dong

Wang

et al. 2020

Plants

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“…; Vijayalakshmi et al . ). In the rhizosphere, roots acquire N mostly in the form of ammonium (NH 4 + ) and nitrate (NO 3 − ), combined with the action of external and internal signals that are often associated with local and systemic signalling processes (Gojon et al .…”

Section: Introductionmentioning

confidence: 99%

Physiological dissection revealed that both uptake and assimilation are the major components regulating different growth responses of two tobacco cultivars to nitrogen nutrition

Fan

et al. 2017

Plant Biol J

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K326 and HD represent major tobacco cultivars in China, which required large N fertiliser input but at different application rates. To understand primary components affecting tobacco N use physiology, we adopted these two varieties as valuable genetic material to assess their growth response to N nutrition. We established a hydroponic culture system to grow plants supplied with different N regimes. Plant biomass, N, ammonium, nitrate, arginine, GS and NR activity, N transfer and use efficiency as well as root uptake were examined. Our data revealed the preference of K326 and HD to utilise nitrate or ammonium nitrate but not ammonium alone, with 2 mm N supply probably sufficient and economical to achieve good biomass production at the vegetative stage. Moreover, both varieties were very sensitive to ammonium, perhaps due to lack of or abnormal signalling related to nitrate and/or arginine rather than impairment of N acquisition and initial assimilation; this was supported by measurements of the plant content of N, ammonium and activities of GS and NR. Notably, short-term N root influx studies identified differential uptake kinetics of K326 and HD, with distinct affinities and transport rates for ammonium and nitrate. The data suggest that the growth adaptation of K326 or HD to higher or lower N may be ascribed to different competences for effective N uptake/translocation and assimilation. Thus, our work provides valuable information to prompt deeper investigation of the molecular basis controlling plant N use efficiency.

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“…Around 800 rice genotypes under low and recommended nitrogen were characterizedusing agro morphological, yield and N related traits/parameters in IIRR farm for two consecutive seasons. Wide variation was observed for all the traits/parameters comprising morphological, yield components, nitrogen content and NUE indicators under low and recommended N. A sub set of genotypes analysed for panicle topology also showed a wide genetic variability but for grain filling % of secondary branches (Vijayalakshmi et al 2015;Rao et al 2018).…”

Section: Identification Of Useful Germplasmmentioning

confidence: 93%

Breeding rice for nitrogen use efficiency

Neeraja¹,

Voleti²,

Subrahmanyam³

et al. 2019

IJGPB

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The aim of present investigation was to study the gross morphology and incidence of canine mammary tumors (CMTs) based on age, sex, breed, reproductive status and location along with histopathological classification and immunohistochemical characteristics. A total of 56 CMTs samples were collected from 49 cases of dogs. Gross morphology was studied in 26 cases of canine mammary tumors. For histopathological classification, samples were fixed in 10% formalin, embedded in paraffin and sections (5 μm thick) obtained from each was stained with HandE stain. Immuno-histochemistry was carried out by using p63 antibody to confirm the histopathological types of CMTs. Malignant tumors and benign tumors were mostly observed in older dogs. Among 9 breeds affected, the highest incidence was recorded in a German shepherd. Caudal abdominal pair was most commonly affected. Most of the cases were observed in intact female dogs, except for one male dog. The tumors were oval and round in shape with 30–2000 g weight, soft to hard in consistency and grayish white cut surface. Out of 56 CMTs, the highest incidence was found of malignant neoplasms (36/56, 64.28%), followed by benign neoplasms (10/56, 17.85%) and non-neoplastic proliferation hyperplasia/dysplasia (10/56, 17.85%). Complex carcinoma, carcinoma, and malignant myoepithelioma and malignant myoepithelioma were confirmed by p63 antibody. In these neoplasms, myoepithelial cells showed strong immunoreactivity with p63.Development of nitrogen use efficient (NUE) rice varieties is inevitable for sustainability of environmental friendly and economical agricultural practices. Several management practices are being studied for increasing efficiency of spatial and temporal inputs of N under National Innovations for Climate Resilient Agriculture (NICRA). Attempts are being made to develop NUE rice varieties with multidisciplinary approach and conventional selection along with mapping and next generation sequencing strategies. Around 800 rice genotypes were characterized under low and recommended nitrogen for two consecutive seasons and the promising donors further evaluated to identify consistent NUE rice genotypes. Several mapping populations were developed using the NUE donors and popular rice varieties. QTL/genomic regions were identified for yield under low N using biparental and association mapping. Using minimum marker set of 50 rice SSR markers, 12 genomic regions were identified for yield and yield associated traits under low nitrogen. Several promising recombinants of yield and NUE were identified and these breeding lines were evaluated under multi-locations and stable performers were identified under AICRIP Trial - Evaluation of Radiation and Nitrogen use efficient promising rice genotypes -Plant Physiology during Kharif 2016, 2017 and 2018. As nitrogen is the building block of biomass, an optimum N is required for realizing the yield. The strategy should be to maximise uptake and improve utilization, so that remobilization of N to yield is achieved under low N.

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Biochemical and physiological characterization for nitrogen use efficiency in aromatic rice genotypes

Cited by 46 publications

References 75 publications

Variations in Nitrogen Metabolism are Closely Linked with Nitrogen Uptake and Utilization Efficiency in Cotton Genotypes under Various Nitrogen Supplies

Variations in Nitrogen Metabolism are Closely Linked with Nitrogen Uptake and Utilization Efficiency in Cotton Genotypes under Various Nitrogen Supplies

Physiological dissection revealed that both uptake and assimilation are the major components regulating different growth responses of two tobacco cultivars to nitrogen nutrition

Breeding rice for nitrogen use efficiency

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