Biochemical characterization of microalgae collected from north east region of India advancing towards the algae‐based commercial production

Abstract

Selection of suitable strain of microalgae is the crucial factor for large-scale production of algae-based products. Efforts have been made here for isolation, identification and biochemical characterization of five microalgae strains collected from Tripura (a small state in north-eastern region of India). Two Chlorococcum sp. (NITAAP008 and NITAAP019) demonstrate their high lipid (15-24%), equal amounts of carbohydrate and protein (35-40%), with specific growth rate of 0.13 day À1 . These strains are potential resource for biofuel production. After lipid extraction, remaining biomass can be used as source of carbohydrate for the production of other biofuels. One isolated strain is identified as Chlorella sp. (NITAAP009) and shows 22-33% carbohydrate, 41-50% protein and 5% chlorophyll with specific growth rate of 0.125 day À1 . Another Chlorella sp. (NITAAP011) isolated from lake area exhibits significant chlorophyll (5-6.4%), 30-50% carbohydrate, 48-60% protein and low lipid (1-10%) with lower specific growth rate (0.10 day À1 ). Both strains are having industrial competence for chlorophyll production due to their synthesizing ability of significant amount of chlorophyll (5-6.4%). The last one, Korshikoviella sp. (NITAAP017), has 15-18% lipid, 22-34% carbohydrate, 30-43% protein and 3-4% chlorophyll with specific growth rate of 0.12 day À1 and can be used for food supplement production or lipid synthesis.

Polar lipidomic profile shows Chlorococcum amblystomatis as a promising source of value-added lipids

“…Chlorococcum amblystomatis is a green microalga with great potential to be a sustainable resource of commercially important essential lipids 1 , 2 , 29 , with promising applications in several industrial sectors 32 , 33 . In our work, the lipid extracts represented 20.77 ± 0.57%, of the dry weight of the biomass, which is consistent with the lipid content reported for Chlorococcum (8.71–32.3%) 10 , 11 , 18 – 20 .…”

“…Chlorococcum sp. has been described as a useful tool in carbon sequestration, lipid flocculation in wastewater 30 and carotenoid production 31 , a good source for the production of biodiesel 32 and useful for the food industry 33 , 34 . The production of lutein from Chlorococcum citriforme has already been patented 35 .…”

“…The production of lutein from Chlorococcum citriforme has already been patented 35 . Regarding its biochemical composition, a total biomass dry weight content of proteins of 13.08–40% 32 , 36 , carbohydrates of 3.42–40% 32 , 36 , chlorophylls of 0.4–1.5% 32 , carotenoids of 3.2–12.6 mg/g 37 and lipids of 8.71–32.3% 32 , 36 , 38 – 40 has been described. In addition to its high lipid content, the FA composition of the Chlorococcum strains studied to date, included good amounts of omega-3 fatty acids 33 suggesting the possibility of added-value lipids that needs to be explored.…”

“…PUFAs were already described in Chlorococcum sp . 32 , 33 , 38 , 39 , however, the polar lipidome of this microalga remains to be described, preventing its full exploitation. Thus, to fill this gap, in the present work, we have analysed and characterized in detail the polar lipidome of Chlorococcum amblystomatis , cultivated in autotrophic conditions, using a lipidomic approach based on the hydrophilic interaction liquid chromatography-electrospray ionization-high resolution mass spectrometry (HILIC–HR–ESI–MS).…”

An Emerging Aquatic Green Gold for Food and Medicine: A Review of Algae From North East India

“…strains including NITAAP009 and NITAAP011 from Tripura demonstrated chlorophyll 5%, 5-6.4%, carbohydrate 22-33%, 30-50%, and protein 41-50%, 48-60% respectively [57]. Former strain show higher specific growth rate of 0.125 d-1 than the later one with 0.10 d-1 [57]. The growth profile of Chlorella sp.…”