Biochemical Characterization of the Staphylococcus aureus PcrA Helicase and Its Role in Plasmid Rolling Circle Replication

Chang, Tseh-Ling; Naqvi, Asma; Anand, Syam P.; Kramer, M. Gabriela; Munshi, Rajan; Khan, Saleem A.

doi:10.1074/jbc.m207383200

Cited by 41 publications

(75 citation statements)

References 34 publications

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“…PcrA from S. aureus is able to hydrolyze ATP, dATP, dGTP, dCTP, and TTP, and its NTPase activity is increased in the presence of either ssDNA or RepC covalently bound to the oriC of pT181 (169). In addition, the unwinding of supercoiled plasmid pT181 is only achieved when RepC is covalently attached to the origin of replication and in the presence of ATP in the reaction (169).…”

Section: Role Of Superfamily Group 1 Helicases and Their Interaction mentioning

confidence: 99%

“…In addition, the unwinding of supercoiled plasmid pT181 is only achieved when RepC is covalently attached to the origin of replication and in the presence of ATP in the reaction (169). PcrA is only capable of unwinding a dsDNA when there is a 3′ or a 5′ single-stranded tail exposed.…”

Section: Role Of Superfamily Group 1 Helicases and Their Interaction mentioning

confidence: 99%

“…PcrA is only capable of unwinding a dsDNA when there is a 3′ or a 5′ single-stranded tail exposed. Hence, PcrA from S. aureus has a bipolar 3′-5′ and 5′-3′ helicase activity (169,170). Two hypotheses have been postulated for the direction of unwinding achieved by PcrA.…”

Section: Role Of Superfamily Group 1 Helicases and Their Interaction mentioning

confidence: 99%

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Plasmid Rolling-Circle Replication

et al. 2015

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Plasmids are DNA entities that undergo controlled replication independent of the chromosomal DNA, a crucial step that guarantees the prevalence of the plasmid in its host. DNA replication has to cope with the incapacity of the DNA polymerases to start de novo DNA synthesis, and different replication mechanisms offer diverse solutions to this problem. Rolling-circle replication (RCR) is a mechanism adopted by certain plasmids, among other genetic elements, that represents one of the simplest initiation strategies, that is, the nicking by a replication initiator protein on one parental strand to generate the primer for leading-strand initiation and a single priming site for lagging-strand synthesis. All RCR plasmid genomes consist of a number of basic elements: leading strand initiation and control, lagging strand origin, phenotypic determinants, and mobilization, generally in that order of frequency. RCR has been mainly characterized in Gram-positive bacterial plasmids, although it has also been described in Gram-negative bacterial or archaeal plasmids. Here we aim to provide an overview of the RCR plasmids' lifestyle, with emphasis on their characteristic traits, promiscuity, stability, utility as vectors, etc. While RCR is one of the best-characterized plasmid replication mechanisms, there are still many questions left unanswered, which will be pointed out along the way in this review.

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Section: Role Of Superfamily Group 1 Helicases and Their Interaction mentioning

confidence: 99%

Section: Role Of Superfamily Group 1 Helicases and Their Interaction mentioning

confidence: 99%

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Plasmid Rolling-Circle Replication

et al. 2015

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“…The PcrA helicase from Bacillus stearothermophilus is a 3′-5′ SF1 DNA helicase involved in DNA repair pathways and the rolling circle replication of some plasmids within that organism (49,50). Monomers of PcrA have been shown to translocate from 3′ to 5′ along ssDNA (24,30,51,52) with a macroscopic rate between 50 nt/s (30) and over 200 nt/s (52) depending upon the solution conditions of the experiment.…”

Section: Sf1 Family Helicasesmentioning

confidence: 99%

All motors have to decide is what to do with the DNA that is given them

Briggs

Fischer

2014

Biomolecular Concepts

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DNA translocases are a diverse group of molecular motors responsible for a wide variety of cellular functions. The goal of this review is to identify common aspects in the mechanisms for how these enzymes couple the binding and hydrolysis of ATP to their movement along DNA. Not surprisingly, the shared structural components contained within the catalytic domains of several of these motors appear to give rise to common aspects of DNA translocation. Perhaps more interesting, however, are the differences between the families of translocases and the potential associated implications both for the functions of the members of these families and for the evolution of these families. However, as there are few translocases for which complete characterizations of the mechanisms of DNA binding, DNA translocation, and DNA-stimulated ATPase have been completed, it is difficult to form many inferences. We therefore hope that this review motivates the necessary further experimentation required for broader comparisons and conclusions.

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“…Physical interaction between an initiator protein and an accessory helicase was experimentally demonstrated between RepC and PcrA in the case of pT181. 14 UvrD and Rep share a high structural homology. The main difference between them, in terms of structure, is the presence of a large disordered C-terminal domain in the UvrD helicase, which is absent in Rep. A similar disordered C-terminal tail is present in the PcrA helicases of gram positive bacteria.…”

Section: Introductionmentioning

confidence: 99%

CTXφ: Exploring new alternatives in host factor-mediated filamentous phage replications

2016

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For a long time Ff phages from Escherichia coli provided the majority of the knowledge about the rolling circle replication mechanism of filamentous phages. Host factors involved in coliphages replication have been fully identified. Based on these studies, the function of Rep protein as the accessory helicase directly implicated in filamentous phage replication was considered a paradigm. We recently reported that the replication of some filamentous phages from Vibrio cholerae, including the cholera toxin phage CTXf, depended on the accessory helicase UvrD instead of Rep. We also identified HU protein as one of the host factors involved in CTXf and VGJf replication. The requirement of UvrD and HU for rolling circle replication was previously reported in some family of plasmids but had no precedent in filamentous phages. Here, we enrich the discussion of our results and present new preliminary data highlighting remarkable divergence in the lifestyle of filamentous phages.

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Biochemical Characterization of the Staphylococcus aureus PcrA Helicase and Its Role in Plasmid Rolling Circle Replication

Cited by 41 publications

References 34 publications

Plasmid Rolling-Circle Replication

Plasmid Rolling-Circle Replication

All motors have to decide is what to do with the DNA that is given them

CTXφ: Exploring new alternatives in host factor-mediated filamentous phage replications

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