Biochemical Characterization of the Suberization-Associated Anionic Peroxidase of Potato

Bernards, Mark A.; Fleming, Warren D.; Llewellyn, David B.; Priefer, Ronny; Yang, Xiaolong; Sabatino, Anita; Plourde, Guy L.

doi:10.1104/pp.121.1.135

Cited by 181 publications

(103 citation statements)

References 49 publications

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“…The higher PPO activity might be associated with better rooting ability in treated cuttings, which was also consistent with data reported by Rout [32,52]. POD are known to be involved in auxin metabolism as well as lignification processes in the cell wall in the presence of phenol [53,54], and our data showed that the decrease of POD activity in NAA-treated tissues ( Figure 3B of adventitious roots in NAA-treated hypocotyls [44]. Moncousin et al [55] reported that low POD corresponded to high IAA levels before the visible appearance of roots.…”

Section: Discussionsupporting

confidence: 79%

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

Shao¹,

Yang²,

Fang³

et al. 2017

Preprint

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In the present study, an attempt was made to induce rooting from single-node cuttings of hybrid aspen (Populus tremula L. ×P. tremuloides Michx.) with different concentrations of IAA, IBA and NAA during rooting. Among the three auxins used, NAA showed more effective induction on rooting as compared to IAA and IBA at the whole level. Thereafter, NAA was used further in experiments for anatomical and biochemical investigation. The results showed that it took 12 days from the differentiation of primordium to the appearance of young adventitious roots with NAA application. It was found that endogenous IAA, ZR and GA3 levels increased, but ABA decreased in cuttings with 0.54 mM NAA treatment. In contrast to the endogenous IAA level, NAA had negative effect on IAA-oxidase (IAAO) activity. Similarly, the decreased peroxidase (POD) activity, consistent with down-regulation of expressed levels of POD1 and POD2, was observed in NAAtreated cuttings. Whereas, NAA resulted in a higher activity in polyphenol oxidase (PPO) compared to the control cuttings. Collectively, the study highlighted that 0.54 mM NAA is efficient on rooting in hybrid aspen, and its effect on metabolic changes during rooting is discussed, which provide valuable information for propagating hybrid aspen.

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Section: Discussionsupporting

confidence: 79%

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

Shao¹,

Yang²,

Fang³

et al. 2017

Preprint

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“…Peroxidases are also involved in suberin formation through a similar process (Bernards et al 1999;Keren-Keiserman et al 2004). …”

Section: Lignification and Suberisationmentioning

confidence: 99%

Peroxidases have more functions than a Swiss army knife

et al. 2005

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Plant peroxidases (class III peroxidases) are present in all land plants. They are members of a large multigenic family. Probably due to this high number of isoforms, and to a very heterogeneous regulation of their expression, plant peroxidases are involved in a broad range of physiological processes all along the plant life cycle. Due to two possible catalytic cycles, peroxidative and hydroxylic, peroxidases can generate reactive oxygen species (ROS) ( • OH, HOO• ), polymerise cell wall compounds, and regulate H 2 O 2 levels. By modulating their activity and expression following internal and external stimuli, peroxidases are prevalent at every stage of plant growth, including the demands that the plant meets in stressful conditions. These multifunctional enzymes can build a rigid wall or produce ROS to make it more flexible; they can prevent biological and chemical attacks by raising physical barriers or by counterattacking with a large production of ROS; they can be involved in a more peaceful symbiosis. They are finally present from the first hours of a plant's life until its last moments. Although some functions look paradoxical, the whole process is probably regulated by a fine-tuning that has yet to be elucidated. This review will discuss the factors that can influence this delicate balance.Keywords Evolution . ROS . (abiotic and biotic) stress . Cell wall loosening and cross-linking . Senescence . Fruit ripening . Symbiosis Multigenic family, evolution and homologyHeme peroxidases specific to plants belong to a superfamily that contains three different classes of peroxidases Communicated by P. Kumar

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“…Lignification near the site of injury is a defense response of plants after wounding (Toivonen and Brummell, 2008;Vance et al, 1980). Strengthening of the plant cell wall induced by POD was reported in potatoes (Bernards and Razem, 2001;Bernards et al, 1999), beet root and sugar beet (Waldron et al, 1997), and Chinese water chestnut (Parr et al, 1996). In addition, no meristematic tissue or periderm layers were found in young, peeled galangal rhizomes after 72 h. As a result, POD may be involved in lignin synthesis via strengthening the cell walls of parenchyma cells in the exterior tissue of the galangal rhizome (Fig.…”

Section: Browning Of Fresh-cut Galangalmentioning

confidence: 99%

Enzymatic Browning of Fresh-cut Galangal (Alpinia siamense K. Schum) and its Relationship to Oxidative Enzymes

Chinwang

Siriphanich

Chairat

2011

J. Japan. Soc. Hort. Sci.

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The role of phenylalanine ammonialyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD) in browning of fresh-cut galangal (Alpinia siamense K. Schum, 'Kha Ta Daeng') rhizome was investigated by determining changes in colour, total phenolic content (TPC), and the activities of these enzymes in the exterior (subepidermis and cortex) and the interior (vascular) tissues within 24 h under ambient conditions (29 ± 1°C and 86 ± 2% RH) after wounding. Browning of the peeled (exterior) and the cut (interior) surface of the rhizome increased progressively after wounding, but the peeled surface showed more browning than the cut surface. In the exterior tissue, TPC increased slightly from 190 to 220 mg gallic acid equivalent/100 g FW over the time of peeling, while PAL activity significantly increased by 37% of the initial level during the first 6 h after peeling, and declined thereafter. TPC in the interior tissue showed the same trend as the exterior tissue with a 10% lower in quantity, while PAL activity in the interior tissue only slightly increased. Changes in TPC and PAL activity in both exterior and interior tissues showed no significant correlations to any of the browning parameters over time (r = 0.16 to 0.48 and r = 0.01 to 0.50, respectively). PPO activity in the exterior tissue increased by 30% of the initial value during the first 6 h after peeling coupled with a slight increase in surface browning of fresh-cut galangal, while the POD activity remained unchanged. As browning progressed, both PPO and POD activities markedly increased in the exterior tissue and showed significant correlations to browning parameters, except for hue angle (r = 0.66 to 0.73 and r = 0.87 to 0.92, respectively). Histochemical assay for PPO showed that the site of browning began on the peeled surface, then gradually progressed to the cut surface, while that for POD occurred immediately on the entire wounded surface and no browning progression was observed, indicating that browning of fresh-cut galangal was likely mediated by PPO. However, POD may likely be involved in the lignification process in response to wounding because, from a microscopic examination, lignin was deposited on the parenchyma cell walls of the wounded exterior of the galangal rhizome.

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Biochemical Characterization of the Suberization-Associated Anionic Peroxidase of Potato

Cited by 181 publications

References 49 publications

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

Peroxidases have more functions than a Swiss army knife

Enzymatic Browning of Fresh-cut Galangal (Alpinia siamense K. Schum) and its Relationship to Oxidative Enzymes

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