Biochemical Differentiation: The Biosynthetic Potentialities of Growing and Quiescent Tissue

Krikorian, A. D.; Steward, F. C.

doi:10.1016/b978-0-12-395679-8.50012-1

Cited by 32 publications

(13 citation statements)

References 262 publications

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“…Authors suggest that enzymes involved in anthranol synthesis and dimerization to hypericins are missing. It has been considered for a long time that undifferentiated cells, such a callus or cell suspensions are not able to produce secondary metabolites, unlike differentiated cells and specialized organs (Krikorian and Steward 1969). Zenk (1991) shows that this theory is wrong.…”

Section: Effects Of Ja On Growth and Cell Differentiationmentioning

confidence: 98%

Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones

Gadzovska

Maury

Delaunay

et al. 2007

Plant Cell Tiss Organ Cult

120

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Hypericum perforatum L. cell suspensions were evaluated for their viability, growth, dark gland formation and ability to produce phenylpropanoids and naphtodiantrones after elicitation with different jasmonic acid (JA) concentrations. Phenolic compounds were analyzed by high performance liquid chromatography with diode array detection (HPLC-DAD) and electrospray ionization mass spectrometry (ESI-MS). The activities of two key enzymes of the phenylpropanoid/flavonoid pathways, phenylalanine ammonia lyase (PAL) and chalcone isomerase (CHI) were also monitored to estimate general channeling in the different metabolic pathways. A 6-fold increase of phenolic compounds, flavanols and flavonols after JA elicitation was observed in cells. In contrast, anthocyanins were in lower amounts in JA treated cells suggesting a modification of the channeling in the phenylpropanoid pathway. Similar accumulations with maxima after 4 days of elicitation were found for naphtodianthrones (2.4-fold) such as hypericin and pseudohypericin in cells. At least a 6-8-fold increase of PAL and CHI activities was observed in JA elicited cells confirming a strong activation of the phenylpropanoid pathway. JA elicitation increased production of phenylpropanoids and naphtodianthrones in H. perforatum cell suspension without differentiation of dark glands under 16 h photoperiod.

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Section: Effects Of Ja On Growth and Cell Differentiationmentioning

confidence: 98%

Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones

Gadzovska

Maury

Delaunay

et al. 2007

Plant Cell Tiss Organ Cult

120

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“…SEBS block copolymers are tough, inexpensive, and biocompatible materials. 37,38 . Sudarsan et al demonstrated the use of SEBS to create microfluidic networks by synthesizing melt processable elastomer gels consisting of 9 to 33 wt % SEBS mixed with mineral oil.…”

Section: Introductionmentioning

confidence: 99%

Simple replica micromolding of biocompatible styrenic elastomers

et al. 2013

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In this work, we introduce a simple solvent-assisted micromolding technique for the fabrication of high-fidelity styrene-ethylene/butylene-styrene (SEBS) microfluidic devices with high polystyrene (PS) content (42 wt% PS). SEBS triblock copolymers are styrenic thermoplastic elastomers that exhibit both glassy thermoplastic and elastomeric properties resulting from their respective hard PS and rubbery ethylene/butylene fractions. The PS fraction gives SEBS microdevices many of the appealing properties of pure PS devices, while the elastomeric fraction simplifies fabrication of the devices, similar to PDMS. SEBS42 devices have wettable, stable surfaces (both contact angle and zeta potential) that support cell attachment and proliferation consistent with tissue culture dish substrates, do not adsorb hydrophobic molecules, and have high bond strength to wide range of substrates (glass, PS, SEBS). Furthermore, SEBS42 devices are mechanically robust, thermally stable, as well as exhibit low auto-fluorescence and high transmissivity. We characterize SEBS42 surface properties by contact angle measurements, cell culture studies, zeta potential measurements, and the adsorption of hydrophobic molecules. The PS surface composition of SEBS microdevices cast on different substrates is determined by time-of-flight secondary ion mass spectrometry (ToF-SIMS). The attractive SEBS42 material properties, coupled with the simple fabrication method, make SEBS42 a quality substrate for microfluidic applications where the properties of PS are desired but the ease of PDMS micromolding is favoured.

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“…very likely as the result of the presence of high auxin levels incompatible with the expression of ethylene effects. As noted by Krikorian and Steward (1969). 'it is difficult to cause cultured tissues and free cells to recapitulate in isolation the metabolism and biochemistry which they exhibit in the environment of the intact plant'.…”

Section: Discussionmentioning

confidence: 99%

“…The use of such material for studying metabolic reactions similar to those which occur in the whole fruit was considered (Pech et al 1974). However, it is known that the change from organized tissue in a whole plant or organ to unorganized callus tissue or to cell suspensions may affect the biochemistry of the cells (Krikorian and Steward 1969). The principal objective of this present work is to describe the possible alterations induced by the in vitro culture of'Passe Crassane' pears on the activity and nature of amylases.…”

Section: Introductionmentioning

confidence: 98%

Tissue and Cell Culture of ‘Passe Crassane’ Pears: Amylase Pattern of Cultured Tissues Compared with Whole Fruit

Pech

Latché

Fallot

1979

Physiologia Plantarum

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Calluses and cell suspension cultures were initiated from young and fully developed fruits and from stem and petiole tissues of ‘Passe Crassane’ pears. On a dry weight basis, proliferation is significantly higher in tissues from full‐grown fruits and petioles. Cullus tissues grown in vitro have a protein content up to 25 times higher than the initial quiescent organ. Three amylase fractions (2 β amylases E, and E2: 1 α amylase E3) were isolated by gel filtration on Sephadcx G‐100 and isoenzymes revealed in these fractions after polyacrylamide gel electrophoresis. Comparison of amylase activity in actively growing cells and in auxin‐starved cell suspensions shows that β‐amylase activity is mainly a function of cell growth, while α‐amylase activity is more related to the age of the cells. The presence of two isoenzymes in the β‐amylase E2 fraction was found to be highly characteristic of young fruits. The same polymorphism was found in all the strain of tissues thus indicating that tissues grown in vitro retain or revert to a juvenile biochemistry. The growth hormone. 2,4‐dichlorophenoxyacetic acid, used to support cell division, is demonstrated to be responsible for the reversion of the β‐amylase polymorphism from a mature to a juvenile phenotype in mature fruit explants incubated in vitro. The interest of fruit tissue and cell culture for the study of fruit physiology is questioned and discussed.

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Biochemical Differentiation: The Biosynthetic Potentialities of Growing and Quiescent Tissue

Cited by 32 publications

References 262 publications

Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones

Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones

Simple replica micromolding of biocompatible styrenic elastomers

Tissue and Cell Culture of ‘Passe Crassane’ Pears: Amylase Pattern of Cultured Tissues Compared with Whole Fruit

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