Biochemical parameters to assess cell differentiation of Bouvardia ternifolia Schlecht callus

Abstract

Calli derived from leaves and radicles of B. ternifolia were grown on Murashige and Skoog (MS) basal medium, and the effects of different nitrogen sources on the rate of callus growth and on the enzymes related to nitrogen assimilation were studied. Ammonium alone did not support callus growth unless a Krebs-cycle intermediate was added to the medium. The activities of glutamine synthetase (EC 6.3.1.2), glutamate synthase (EC 1.4.7.1), and glutamate dehydrogenase (EC 1.4.1.2) were measured in homogenates of callus grown on media supplied with different nitrogen sources. The results indicate that leaf and root calli have similar levels of these enzymes when grown on MS medium (Murashige and Skoog 1962. Physiol. Plant. 15, 473-497). However, when the calli were supplied with glutamine as the sole nitrogen source, the activity of glutamate synthase increased in leaf callus but was almost completely inhibited in root callus. The results indicate that calli originated from different B. ternifolia tissues do not have the same biochemical dedifferentiated state.

Primary metabolites and organic acid metabolism in apple (Malus sylvestris) fruit callus culture

“…Similarities in the incorporation of 14C compounds as well as in the profile of sugars and amino acids in tomato callus, leaf and fruit explant have been shown (Danek-Jezik and Schmidt 1979;Obato-Sasamoto et a1 1984). Differences in the biochemical properties in the callus cultures and their explants were pointed out in tobacco and potato tissues with respect to the metabolism of 14C glucose and 14C0, (Dejimenez et al 1983;Abou-Mandour and Kaiser 1984).…”

OBTENCIÓN DE DEHIDRODIISOEUGENOL POR DIMERIZACIÓN DE ISOEUGENOL CON CULTIVOS CELULARES DE Bouvardia ternifolia (TROMPETILLA)

“…Las hojas y flores (explantes) se pusieron en una disolución 1:1 de ácido ascórbico y cítrico (100 mg/L). Las hojas se cortaron en segmentos de 1 cm 2 y las flores se cortaron en cuatro segmentos colocándolas en el medio de cultivo Murashige and Skoog (MS) (Sánchez & Fernández, 1983)…”