Biochemical Profile and Enzymatic Activity of Organs of Late-phase Laying Hens Fed Diet With Calcium Anacardate Associated With Citric Acid

Abstract

The aim of this study was to evaluate dietary inclusion of calcium anacardate (CAN) associated or not with citric acid (CA) in late-phase laying hen diets on serum biochemical profile, serum lipid peroxidation (TBARS), enzymatic activity (CAT and SOD) of liver, ovary, magnum and uterus. A total of 432 laying hens with 63 weeks of age were distributed into nine treatments and six replicates. Treatments consisted of: T1 – diet without additives; T2 – diet with 0.25%CAN; T3 – diet with 0.25%CAN + 0.25%CA; T4 – diet with 0.50%CAN; T5 – diet with 0.50%CAN + 0.25%AC; T6 – diet with 0.50%CAN + 0.50%CA; T7 – diet with 0.75%CAN; T8 – diet with 0.75%CAN + 0.25%CA; T9 – diet with 0.75%CAN + 0.50%CA. Lower serum TBARS was observed in birds fed 0.75% CAN and associate with AC (0.50% CAN + 0.50% CA). Dietary inclusion of CAN (0.50% and 0.75%) or associated with CA (0.25% and 0.50%) resulted in lower CAT in the liver and higher enzyme activity in the magnum. SOD activity in magnum was higher in laying hens fed diet without additives and lower values was observed considering increasing level of CAN. Dietary inclusion of CAN (0.75%) and its association with CA (0.50% CAN with 0.50 CA) for late-phase laying hens reduce serum peroxidation. Higher CAT activity was observed in magnum from the level of 0.50% CAN with or without CA. Increasing levels of CAN associated with CA results in lower CAT activity in the liver and SOD activity in reproductive tissues.